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Diagnostic assays with monoclonal antibodies for the human serum parvovirus-like virus (SPLV)

Published online by Cambridge University Press:  19 October 2009

B. J. Cohen ,
P. P. Mortimer  and
M. S. Pereira
B. J. Cohen
Affiliation:
PHLS Virus Reference Laboratory, Central Public Health Laboratory, Colindale Avenue, London NW9
P. P. Mortimer
Affiliation:
PHLS Virus Reference Laboratory, Central Public Health Laboratory, Colindale Avenue, London NW9
M. S. Pereira
Affiliation:
PHLS Virus Reference Laboratory, Central Public Health Laboratory, Colindale Avenue, London NW9
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Monoclonal antibodies to the serum parovirus-like virus (SPLV) were prepared by the hybridoma technique. They provided an antibody reagent which was used to develop solid phase antibody-capture assays for anti-SPLV IgM and IgG and for SPLV antigen. These assays were more sensitive than those based on human convalescent antibody as a reagent, and were more economical in the use of SPLV antigen. Their use enabled the serological responses to SPLV to be studied more fully and their sensitivity revealed the extent of SPLV infection.

SPLV antigen was detected in four patients by both counter-immuno electrophoresis (CIE) and radioimmunoassay (RIA) and in two others by RIA alone. Parvovirus particles were seen in all six by electron microscopy. The anti-SPLV IgM response was measured in patients infected by SPLV. It was strong 5–18 days after the onset of illness, then declined and was only detectable in trace amounts after 6 months. Anti-SPLV IgG was also formed early, and persisted for at least 6 months. In a survey of 310 blood donors anti-SPLV was detected in 134 (43%) by CIE, but in 190 (61 %) by IgG antibody capture RIA.

Type
Research Article
Information
Journal of Hygiene , Volume 91 , Issue 1 , August 1983 , pp. 113 - 130
Copyright
Copyright © Cambridge University Press 1983

References

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