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The development and use of an antibody capture radioimmunoassay for specific IgM to a human parvovirus-like agent

Published online by Cambridge University Press:  25 March 2010

M. J. Anderson ,
L. R. Davis ,
S. E. Jones ,
J. R. Pattison  and
G. R. Serjeant
M. J. Anderson
Affiliation:
King's College Hospital Medical School, Denmark Hill, London SE5 8RX
L. R. Davis
Affiliation:
Departments of Medical Microbiology and Haematology, Denmark Hill, London SE5 8RX
S. E. Jones
Affiliation:
King's College Hospital Medical School, Denmark Hill, London SE5 8RX
J. R. Pattison
Affiliation:
King's College Hospital Medical School, Denmark Hill, London SE5 8RX
G. R. Serjeant
Affiliation:
MRC Laboratories (Jamaica), University of West Indies, Mona, Kingston 7, Jamaica, West Indies
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Summary

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An IgM-antibody capture radioimmunoassay (MACRIA) was developed for the detection of IgM antibody specific for the human parvovirus-like agent B19. Diagnosis of infection with this agent by either antigen detection or antibody seroconversion had been made by counter-current immunoelectrophoresis (CIE) in 18 cases of aplastic crisis occurring in children with homozygous sickle-cell disease. The MACRIA described here gave positive results in 17 of these 18 cases; in the remaining case only an acute specimen taken from the patient during viraemia and late convalescent specimens taken 184 and 247 days after onset of illness were available.

The test was used to investigate 20 further cases of aplastic crisis in which neither viral antigen nor antibody seroconversion could be detected by CIE. Detection of virus-specific IgM permitted diagnosis of infection with this parvovirus-like agent in 17 of these cases. In the remaining three cases only single serum specimens taken late in convalescence, 82 days or more after the onset of symptoms, were available.

In addition to these 34 cases of aplastic crisis in which primary infection with this agent was diagnosed by MACRIA, seven cases of apparent ‘silent’ infection detected by CIE were investigated. The test permitted the discrimination between primary infection and re-exposure to the virus in six of these patients.

The use of this assay has added a considerable weight of evidence implicating primary infection with this parvovirus-like agent as an important cause of aplastic crisis in children with sickle-cell disease. Furthermore, MACRIA permits diagnosis of infection when only single serum specimens taken up to ten weeks after infection are available. Thus the use of this test will significantly facilitate the investigation of other clinical syndromes of presumptive infectious aetiology.

Type
Research Article
Information
Journal of Hygiene , Volume 88 , Issue 2 , April 1982 , pp. 309 - 324
Copyright
Copyright © Cambridge University Press 1982

References

REFERENCES

Anderson, M. J., Davis, L. R., Hodgson, J., Jones, S. E., Murtaza, L., Pattison, J. R., Stroud, C. E. & White, J. M. (1982). The occurrence of infection with a parvovirus-like agent in children with sickle cell anaemia during a two year period. Journal of Clinical Pathology, in press.CrossRefGoogle ScholarPubMed
Cossart, Y. E., Field, A. M., Cant, B. & Widdows, D. (1975). Parvovirus-like particles in human sera. Lancet i, 71–73.Google Scholar
Flehmig, B., Ranks, M., Berthold, H. & Gerth, H.-J. (1979). A solid phase radioimmunoassay for detection of IgM antibodies to hepatitis A virus. Journal of Infectious Diseases 140, 169–175.CrossRefGoogle ScholarPubMed
Mortimer, P. P., Tedder, R. S., Hambling, M. H., Shafi, M. S., Burkhardt, F. & Schilt, U. (1981). Antibody capture radioimmunoassay for anti-rubella IgM. Journal of Hygiene 86, 139–153.CrossRefGoogle ScholarPubMed
Pattison, J. R., Jones, S. E., Hodgson, J., Davis, L. R., White, J. M., Stroud, C. E. & Murtaza, L. (1981). Parvovirus infections and hypoplastic crises in sickle cell anaemia. Lancet i, 664–665.CrossRefGoogle Scholar
Paver, W. K. & Clarke, S. K. R. (1976). Comparison of human fecal and serum parvo-like viruses. Journal of Clinical Microbiology 4, 67–70.CrossRefGoogle ScholarPubMed
Salacinski, P., Hope, J., McClean, C., Clement-Jones, V., Sykes, J., Price, J. & Lowry, P. J. (1979). A new simple method which allows theoretical incorporation of radioiodine into proteins and peptides without damage. Journal of Endocrinology 81, 131–137.Google ScholarPubMed
Schneerson, J. M., Mortimer, P. P. & Vandervelde, E. M. (1980). Febrile illness due to a parvovirus. British Medical Journal 2, 1580.CrossRefGoogle Scholar
Serjeant, G. R., Topley, J. M., Mason, K., Serjeant, B. E., Pattison, J. R., Jones, S. E. & Mohamed, R. (1981). Outbreak of aplastic crises in sickle cell anaemia associated with parvovirus-like agent. Lancet ii, 595–597.CrossRefGoogle Scholar
Tedder, R. S. (1981). Laboratory Investigation of Rubella. No. 16 PHLS Monograph Series (ed. Pattison, J. R.), London: H.M.S.O.Google Scholar
Tedder, R. S. & Wilson-Croome, Ruth (1981). IgM-antibody response to the hepatitis B core antigen in acute and chronic hepatitis B. Journal of Hygiene 86, 163–172.CrossRefGoogle Scholar