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Detection of Legionella pneumophila in environmental water samples using a fluorescein conjugated monoclonal antibody

Published online by Cambridge University Press:  15 May 2009

T. Makin  and
C. A. Hart
T. Makin
Affiliation:
Department of Medical Microbiology, University of Liverpool, P.O. Box 147, Liverpool L69 3BX
C. A. Hart
Affiliation:
Department of Medical Microbiology, University of Liverpool, P.O. Box 147, Liverpool L69 3BX
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Summary

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Sixty-three environmental water samples from various sources were examined for the presence of Legionella pneumophila with a commercially available direct fluorescent monoclonal antibody (GS), an indirect fluorescent antibody test (IFAT) and culture. GS detected L. pneumophila in 94% and 100% of environmental water samples which were culture and IFAT positive for L. pneumophila, respectively. IFAT detected 69% of L. pneumophila culture positive samples. Cultures of L. pneumophila serogroups 1 to 12, 14 and non L. pneumophila bacteria which may be found in water, and bacteria containing non-specific binding proteins, were stained by GS and IFAT. GS identified all serogroups of L. pneumophila and did not cross react with any non-L. pneumophila bacteria. L. pneumophila in environmental samples was easy to detect against a clear dark background when stained with GS.

Type
Special Article
Information
Epidemiology & Infection , Volume 103 , Issue 1 , August 1989 , pp. 105 - 112
Copyright
Copyright © Cambridge University Press 1989

References

REFERENCES

1.HMSO, 1987. Second Report of the Committee of Inquiry into the outbreak of Legionnaires' disease in Stafford in April 1985.Google Scholar
2.Makin, T. Inhibition of Legionella by other organisms. Abtracts of papers presented at the 18th Triennial Conference. Southampton: Institute of Medical Laboratory Sciences, 1986: 9.Google Scholar
3.Gosting, LH, Cabrian, K, Sturge, J, Goldstein, LC. Identification of a species-specific antigen in L. pneumophila by a monoclonal antibody. J Clin Microbiol 1984; 20: 1031–5.CrossRefGoogle Scholar
4.Edelstein, PH, Beer, KB, Sturge, JC, Watson, AJ, Goldstein, LC. Clinical utility of a monoclonal direct fluorescent reagent specific for Legionella pneumophila: comparative study with other reagents. J Clin Microbiol 1985; 22: 419–21.CrossRefGoogle ScholarPubMed
5.Fallon, RJ. Identification of Legionella pneumophila with a commercially available immunofluorescence test. J Clin Pathol 1986; 39: 693–4.CrossRefGoogle ScholarPubMed
6.Edelstein, PH. Improved semiselective medium for isolation of Legionella pneumophila from contaminated clinical and environmental specimens. J Clin Microbiol 1981; 14: 298–303.CrossRefGoogle ScholarPubMed
7.Harrison, TG, Taylor, AG. Diagnosis of Legionella pneumophila infections by means of formolised yolk sac antigens. J Clin Pathol 1982; 35: 211–4.CrossRefGoogle ScholarPubMed
8.Galen, RS, Gambino, SR. Beyond normality: The predictive value and efficiency of medical diagnoses. London: John Wiley and Sons, 1976.Google Scholar
9.Fliermans, CB, Cherry, WB, Orrison, LH, Smith, ST, Tison, DL, Pope, DH. Ecological distribution of Legionella pneumophila. Appl Environ Microbiol 1981; 41: 9–16.CrossRefGoogle ScholarPubMed
10.Dennis, PJ, Taylor, JA, Fitzgeorge, R, Bartlett, C, Barrow, G.Legionella pneumophila in water plumbing systems. Lancet 1982; 1: 949–51.CrossRefGoogle ScholarPubMed
11.HMSO, 1988. The control of Legionellae in health care premises: A code of practice.Google Scholar
12.Walker, RA. Testing for Legionella bacteria. Occup Health Rev 1988; 16: 17–8.Google Scholar
13.Meigh, RE, Makin, T, Scott, MH, Hart, CA. Legionella pneumophila sero-group 12 pneumonia in a renal transplant recipient: case report and environmental observations. J Hosp Infect 1989; 13: 315–9.CrossRefGoogle Scholar
14.Saravolatz, LD, Pohlod, D, Helzer, K, Berttina, W, Levin, N. Legionella infections in renal transplant recipients. In: Thornsberry, C, Balows, A, Feeley, JC, Jacubowski, W, eds. Legioneila: Proceedings of the 2nd International Symposium. Washington D.C.: American Society for Microbiology, 1984: 243–3.Google Scholar
15.Kuritsky, JN, Reingold, AL, Hightower, AW, Broome, CV. Sporadic legionellosis in the United States. In: Thornsberry, C, Balows, A, Feeley, JC, Jakubowski, W, eds. Legionelia: Proceedings of the 2nd International Symposium. Washington D.C.: American Society for Microbiology, 1984: 243–5.Google Scholar
16.Benson, RF, Thacker, WL, Wilkinson, HW, Fallon, RJ, Benson, DJ. Legionella pneumophila serogroup 14 isolated from patients with fatal pneumonia. J Clin Microbiol 1988; 26: 382.CrossRefGoogle ScholarPubMed
17.McKinney, MR, Wilkinson, HW, Sommers, HM, et al. Legionella pneurnophila serogroup six: isolation from cases of legionellosis. Identification by immunofluorescence staining and immunological response to infection. J Clin Microbiol 1980; 12: 395–401.CrossRefGoogle ScholarPubMed
18.Goldberg, SJ, Collier, PW, Fallon, RJ et al. Lochgoilhead fever: outbreak of non-pneumonic Legionellosis due to Legionella micdadei. Lancet 1989; 1: 316–8.CrossRefGoogle ScholarPubMed