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Crystal violet reactions of Staphylococcus aureus strains colonizing infants in the first six weeks

Published online by Cambridge University Press:  15 May 2009

S. J. Hudson ,
R. Freeman ,
D. Burdess  and
B. D. Cookson
S. J. Hudson
Affiliation:
Department of Microbiology, Queen Elizabeth Hospital, Gateshead, andMedical School, Newcastle upon Tyne
R. Freeman*
Affiliation:
Department of Microbiology, Queen Elizabeth Hospital, Gateshead, andMedical School, Newcastle upon Tyne
D. Burdess
Affiliation:
Department of Microbiology, Queen Elizabeth Hospital, Gateshead, andMedical School, Newcastle upon Tyne
B. D. Cookson
Affiliation:
Division of Hospital Infection, Central Public Health Laboratory, Colindale, London
*
*Dr R. Freeman, Department of Microbiology. Medical School, Framlington Place. Newcastle upon Tyne NE2 4HH
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Summary

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Nasal colonization with Staphylococcus aureus occurred in 18% of babies leaving a maternity unit and had risen to 40% by 6 weeks after birth. S. aureus was first acquired by 34·5% of babies after discharge. Female infants were more likely to be colonized than males. Colonization was not significantly different between babies receiving standard postnatal care and those nursed on the Special Care Baby Unit.

Crystal violet (CV) tests showed that purple-reacting isolates accounted for approximately 60% of strains, whether first detected at hospital discharge or subsequently acquired. Purple-reacting strains, once acquired, were significantly better able to persist than non purple-reacting strains and formed a cumulatively higher proportion of the strains isolated at 6 weeks after birth than at hospital discharge. CV purple-reactions were significantly associated with lysis by phages of groups III and I and non-purple-reactions were significantly associated with lysis by phages of group II and/or 94/96.

Maternity units remain a significant route whereby strains of S. aureus with some characteristics associated with a hospital origin gain access to the community.

Type
Research Article
Information
Epidemiology & Infection , Volume 110 , Issue 1 , February 1993 , pp. 79 - 86
Copyright
Copyright © Cambridge University Press 1993

References

REFERENCES

1.Vesikari, T, Isolauri, E, Tuppurainen, N, et al. Neonatal septicaemia in Finland 1981–85. Acta Paediatr Scan 1989; 78: 44–50.CrossRefGoogle ScholarPubMed
2.Kaplan, MH. Chmel, H, Hsieh, H-S, Stephens, A. Brinsko, V. Importance of exfoliatin toxin A production by Staphylococcus aureus strains isolated from clustered epidemics of neonatal pustulosis. J Clin Microbiol 1986; 23: 83–91.CrossRefGoogle ScholarPubMed
3.Dancer, SJ, Simmons, NA, Poston, SM, Noble, WC. Outbreak of staphylococcal scalded skin syndrome among neonates. J Infect Dis 1988; 16: 87–103.Google ScholarPubMed
4.Freeman, R, Hudson, SJ, Burdess, D. Crystal violet reactions of fresh clinical isolates of Staphylococcus aureus from two British hospitals. Epidemiol Infect 1990; 105: 493–500.CrossRefGoogle ScholarPubMed
5.Blair, JE, Williams, REO. Phage typing of staphylococci. Bull Wld Hlth Org 1961; 24: 771–84.Google ScholarPubMed
6.Hargiss, C, Larson, E. The epidemiology of Staphylococcus aureus in a newborn nursery from 1970 through 1976. Pediatrics 1978; 61, 348–53.Google Scholar
7.Gezon, HM, Thompson, DJ, Yee, RB, Rogers, KD. Host factors in infection and disease in the newborn. In: Skin bacteria and their role in infection. New York: McGraw-Hill, 1965.Google Scholar
8.Jellard, J. Umbilical cord as a reservoir of infection in a maternity unit. BMJ 1957: 1: 925–8.CrossRefGoogle Scholar
9.Laursen, H. Bacteriological colonisation of infants and mothers in a maternity unit. Acta Obstet Gynecol Scand 1963; 42: 43–64.CrossRefGoogle Scholar
10.Wolinsky, E, Gonzaga, AJ, Mortimer, EA. The mother as a source of neonatal staphylococci. New Eng J Med 1962; 267: 535–8.CrossRefGoogle ScholarPubMed
11.Williams, REO. Carriage of staphylococci in the newborn. Lancet 1961: ii: 173–5.CrossRefGoogle Scholar
12.Hurst, V. Resistance of staphylococci carried by infants and young children. J Lab Clin Med 1958: 52: 254–8.Google ScholarPubMed
13.Rycroft, JA. Williams, REO. Penicillin-resistant staphylococci in normal young children. Proc Roy Soc Med 1960: 53: 258–60.Google ScholarPubMed