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Correlation between uropathogenic properties of Escherichia coli from urinary tract infections and the antibody-coated bacteria test and comparison with faecal strains

Published online by Cambridge University Press:  25 March 2010

Heather J. L. Brooks ,
Barbara A. Benseman ,
Jayne Peck  and
K. A. Bettelheim
Heather J. L. Brooks
Affiliation:
Department of Microbiology and Genetics, Massey University, Palmerston North, New Zealand
Barbara A. Benseman
Affiliation:
Department of Microbiology and Genetics, Massey University, Palmerston North, New Zealand
Jayne Peck
Affiliation:
Department of Microbiology and Genetics, Massey University, Palmerston North, New Zealand
K. A. Bettelheim
Affiliation:
National Health Institute, Wellington, New Zealand
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Summary

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Strains of Escherichia coli isolated from adult females with symptomatic urinary tract infection were found to possess the following properties significantly more frequently than faecal strains: (i) high K-antigen titre; (ii) haemolysin; (iii) type 1 pili; (iv) mannose-resistant haemagglutination; (v) fermentation of dulcitol and salicin; (vi) O serotype 2, 6 and 75; (vii) H serotype 1. E. coli isolated from urine specimens containing significant numbers of antibody-coated bacteria were richer in these seven properties than strains from urines without detectable antibody-coated bacteria.

The O and H serotypes of E. coli obtained from patients with urinary tract infection in two New Zealand cities were compared with those reported in the world literature and found to be similar.

Type
Research Article
Information
Journal of Hygiene , Volume 87 , Issue 1 , August 1981 , pp. 53 - 61
Copyright
Copyright © Cambridge University Press 1981

References

Barnett, G. R. & Abbott, G. D. (1978). Localisation of gram negative urinary tract infection by immunofluorescence in infants and children. Australian Paediatrics Journal 14, 143–6.Google ScholarPubMed
Brooks, H. J. L., O'Grady, F.. McSherry, M. A. & Cattell. W. R. (1980). Uropathogenic properties of Escherichia coli in recurrent urinary-tract infection. Journal of Medical Microbiology 13, 57–68.CrossRefGoogle ScholarPubMed
Brumfitt, W. & Heptinstall, R. H. (1960). Experimental pyelonephritis: the relationship of bacterial virulence to the establishment of the renal lesion. British Journal of Experimental Pathology 41, 552–8.Google Scholar
Duguid, J. P. (1968). The function of bacterial fimbriae. Archivum Immunologiae et Therapae Experimentalis 16, 173–88.Google ScholarPubMed
Duguid, J. P., Clegg, S. & Wilson, M. I. (1979). The fimbrial and non-fimbrial haemagglutinins of Escherichia coli. Journal of Medical Microbiology 12, 213–27.CrossRefGoogle ScholarPubMed
Duguid, J. P., Smith, I. W., Dempster, G. & Edmunds, P. X. (1955). Non-flagellar filamentous appendages (fimbriae) and haemagglutinating activity in Bacterium coli. Journal of Pathology and Bacteriology 70, 335–48.CrossRefGoogle ScholarPubMed
Edén, C. S. & Hansson, H. A. (1978). Escherichia coli pili as possible mediators of attachment to human urinary tract epithelial cells. Infection and Immunity 21. 229–37.CrossRefGoogle ScholarPubMed
Edwards, P. R. & Ewing, W. H. (1972). The genus Escherichia. In Identification of Enterobacteriaceae, 3rd ed. p. 70. Minneapolis: Burgess.Google Scholar
Fairley, K. F., Carson, N. E., Gutch, R. C.Leighton, P., Grounds, A. D., Laird, E. C., McCallum, P. H. G., Sleeman, R. I. & O'Keefe, C. M. (1971). Site of infection in acute urinary-tract infection in general practice. Lancet 2, 615–18.CrossRefGoogle ScholarPubMed
Fang, L. S., Tolkoff-Rubin, N. E. & Rubin, R. H. (1978). Efficacy of single-dose and conventional amoxicillin therapy in urinary-tract infection localized by the antibody-coated bacteria technic. New England Journal of Medicine 298, 413–16.CrossRefGoogle ScholarPubMed
Forsum, V., Fritjofsson, A., Frodin, L., Hjelm, E. & Jonsell, G. (1978). A clinical evaluation of a test for antibody-coated bacteria in the urine. Scandinavian Journal of Urology and Nephrology 12, 45–8.Google ScholarPubMed
Glynn, A. A. & Howard, C. J. (1970). The sensitivity to complement of strains of Escherichia coli related to their K antigens. Immunology 18, 331–46.Google ScholarPubMed
Grüneberg, R. N. & Bettelheim, K. A. (1969). Geographical variation in serological types of urinary Escherichia coli. Journal of Medical Microbiology 2, 219–24.CrossRefGoogle ScholarPubMed
Guze, L. B., Montgomerie, J. Z., Potter, C. S. & Kalmanson, G. M. (1973). Pyelonephritis. XVI. Correlates of parasite virulence in acute ascending Escherichia coli pyelonephritis in mice undergoing diuresis. Yale Journal of Biology & Medicine 46, 203–11.Google ScholarPubMed
Hellerstein, S., Kennedy, E., Nussbaum, L. & Rice, K. (1978). Localisation of the site of urinary tract infections by means of antibody-coated bacteria in the urinary sediments. Journal of Pediatrics 92, 188–93.CrossRefGoogle ScholarPubMed
Howard, C. J. & Glynn, A. A. (1971). The virulence for mice of strains of Escherichia coli related to the effects of K antigens on their resistance to phagocytosis and killing by complement. Immunology 20, 767–77.Google Scholar
Jones, S. R., Smith, J. W. & Sanford, J. P. (1974). Localisation of urinary-tract infections by detection of antibody-coated bacteria in urine sediment. New England Journal of Medicine 209, 591–3.CrossRefGoogle Scholar
Kalmanson, G. M., Turck, M., Harwick, H. J. & Guze, L. B. (1975). Urinary-tract infection: localisation and virulence of Escherichia coli. Lancet 1, 134–6.CrossRefGoogle ScholarPubMed
Meekin, G. E., Bettelheim, K. A. & Bacon, D. F. (1979). Serotypes of antibiotic resistant Escherichia coli isolated from the sewage of Palmerston North (New Zealand). Journal of Hygiene 82, 443–52.CrossRefGoogle ScholarPubMed
Minshew, B. H., Jorgensen, J., Counts, G. W. & Falkow, S. (1978). Association of haemolysin production, haemagglutination of human erythrocytes, and virulence for chicken embryos of extraintestinal Escherichia coli isolates. Infection and Immunity 20, 50–4.CrossRefGoogle ScholarPubMed
Mundt, K. A. & Polk, B. F. (1979). Identification of site of urinary tract infections by antibody-coated bacteria assay. Lancet 2, 1172–5.CrossRefGoogle ScholarPubMed
Peddie, B. A. & Little, P. J. (1978). Serogroups of Escherichia coli in symptomatic and asymptomatic urinary tract infections in Christchurch. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene I. Abt. Orig. A 240, 320–5.Google ScholarPubMed
Riedasch, G., Ritz, E., Möhring, K. & Bommer, J. (1978). Antibody coating of urinary bacteria: relation to site of infection and invasion of uroepithelium. Clinical Nephrology 10, 239–44.Google ScholarPubMed
Rumans, L. W. & Vosti, K. L. (1978). The relationship of antibody-coated bacteria to clinical syndromes. Archives of Internal Medicine 138, 1077–81.CrossRefGoogle ScholarPubMed
Schaeffer, A. J., Amundsen, S. K. & Schmidt, L. N. (1979). Adherence of Escherichia coli to human urinary tract epithelial cells. Infection and Immunity 24, 753–9.CrossRefGoogle ScholarPubMed
Siegel, S. (1956). Chi Square test for two independent samples. Mann-Whitney U. test. In Non-parametric statistics for the Behavioural Sciences, pp. 104–11 and 116–27. McGraw-Hill Kogakusha Ltd.Google Scholar
Sietzen, W. (1979). Typisierung von Escherichia coli-O-und-K Antigenen bei Patienten mit Harnwegsinfektionen. Infection 7, Suppl. 2, 197–206.CrossRefGoogle Scholar
Smith, J. W., Jones, S. R. & Kaijser, B. (1977). Significance of antibody-coated bacteria in urinary sediment in experimental pyelonephritis. Journal of Infectious Diseases 135, 577–81.CrossRefGoogle ScholarPubMed
Thomas, V., Shelokov, A. & Forland, M. (1974). Antibody-coated bacteria in the urine and the site of urinary-tract infection. New England Journal of Medicine 290, 588–90.CrossRefGoogle ScholarPubMed
Thomas, V., Forland, M., LeStourgeon, D. & Shelokov, A. (1978). Antibody-coated bacteria in persistent and recurrent urinary tract infections. Kidney International 14, 607–13.CrossRefGoogle ScholarPubMed
van den Bosch, J. F., de Graaff, J. & Maclaren, D. M. (1979). Virulence of Escherichia coli in experimental haematogenous pyelonephritis in mice. Infection and Immunity 25, 68–74.CrossRefGoogle ScholarPubMed
Wong, W. T. & Bettelheim, K. A. (1976). Serotypes of Escherichia coli from urinary tract infections in Hong Kong. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene, I. Abt. Orig. A 236, 481–6.Google ScholarPubMed