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A comparison of three vaccines against respiratory syncytial virus in calves

Published online by Cambridge University Press:  19 October 2009

E. J. Stott ,
L. H. Thomas ,
G. Taylor ,
A. P. Collins ,
J. Jebbett  and
S. Crouch
E. J. Stott
Affiliation:
Agricultural and Food Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berkshire
L. H. Thomas
Affiliation:
Agricultural and Food Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berkshire
G. Taylor
Affiliation:
Agricultural and Food Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berkshire
A. P. Collins
Affiliation:
Agricultural and Food Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berkshire
J. Jebbett
Affiliation:
Agricultural and Food Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berkshire
S. Crouch
Affiliation:
Agricultural and Food Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berkshire
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Summary

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An inactivated vaccine against respiratory syncytial virus (RSV) was compared with two live vaccines. The inactivated (GC) vaccine consisted of glutaraldehyde-fixed bovine nasal mucosa cells persistently infected with RSV and emulsified with oil adjuvant. The live vaccines were a modified virus (MV) derived from a bovine strain of RSV and a temperature-sensitive mutant (ts-1) derived from a human strain. The GC vaccine was inoculated subcutaneously into 12 calves and the live vaccines intramuscularly into eight calves each. Nine unvaccinated calves acted as controls. The vaccines were administered in two doses 3 weeks apart and all calves were challenged intranasally with 2 × 107 p.f.u. of bovine RSV 3 weeks after the second dose.

At the time of challenge calves given GC, MV and ts-1 vaccines had mean serum neutralizing antibody titres of 25, 19 and 2 respectively; mean titres of IgG1 antibody by radioimmunoassay were log10 4·5, 1·3 and 2·6 respectively and mean zone areas by single radial haemolysis (SRH) were 107, 27 and 36 mm2 respectively.

Eleven of 12 calves given GC vaccine were completely protected against challenge but all control animals and those given the two live vaccines were infected. The mean peak titre of virus in nasal swabs of control calves was 3.0 log10 p.f.u./ml and the mean duration of virus shedding was 6·8 days. Both these parameters were significantly reduced in animals given MV and ts-1 vaccines: mean peak titres were 2·1 and 2·4 log10 p.f.u./ml and mean duration of shedding was 3·4 and 3·3 days respectively.

Thus, protection correlated better with RSV antibody detected by radio-immunoassay and SRH than with neutralizing antibody. These results are discussed in relation to the possible mechanism by which protection was mediated.

Type
Research Article
Information
Journal of Hygiene , Volume 93 , Issue 2 , October 1984 , pp. 251 - 261
Copyright
Copyright © Cambridge University Press 1984

References

Beem, M. (1967). Repeated infections with respiratory syncytial virus. Journal of Immunology 98, 1115–1122.CrossRefGoogle ScholarPubMed
Belshe, R. B., Van Voris, L. P. & Mufson, M. A. (1982). Parenteral administration of live respiratory syncytial virus vaccine: results of a field trial. Journal of Infectious Diseases 145, 311–319.CrossRefGoogle ScholarPubMed
Buynak, E. B., Weibel, R. E., McLean, A. A. & Hilleman, M. R. (1978). Live respiratory syncytial virus vaccine administered parenterally. Proceedings of the Society for Experimental Biology and Medicine 157, 636–642.CrossRefGoogle ScholarPubMed
Chanock, R. M. & Parrott, R. H. (1965). Acute respiratory disease in infancy and childhood: present understanding and prospects for prevention. Pediatrics 36, 21–39.CrossRefGoogle ScholarPubMed
Fernie, B. F. & Gerin, J. L. (1982). Immunochemical identification of viral and nonviral proteins of the respiratory syncytial virus virion. Infection and Immunity 37, 243–249.CrossRefGoogle ScholarPubMed
Gharpure, M. A., Wright, P. F. & Chanock, R. M. (1969). Temperature-sensitive mutants of respiratory syncytial virus. Journal of Virology 3, 414–421.CrossRefGoogle ScholarPubMed
Grist, N. R., Ross, C. A. C. & Stott, E. J. (1967). Influenza, respiratory syncytial virus and pneumonia in Glasgow 1962–5. British Medical Journal i, 456–457.CrossRefGoogle Scholar
Hall, C. B. (1980). Prevention of infections with respiratory syncytial virus: the hopes and hurdles ahead. Reviews of Infectious Diseases 2, 384–392.CrossRefGoogle ScholarPubMed
Henderson, F. W., Collier, A. M., Clyde, W. A. & Denny, F. W. (1979). Respiratory syncytial virus infections, reinfections and immunity. New England Journal of Medicine 300, 530–534.CrossRefGoogle ScholarPubMed
Holzhauer, C. (1978). Bronchopneumonia of yearling cattle. D.V.M. thesis, University of Utrecht.Google Scholar
Holzhauer, C. & Van Nieuwstadt, A. P. K. I. (1976). De etiologische rol van het bovine respiratory syncytial virus bij pinkengriep. Tijdschr Diergeneesk 101, 1023–1031.Google ScholarPubMed
Kapikian, A. Z., Mitchell, R. H., Chanock, R. M., Shvedoff, R. A. & Stewart, C. E. (1969). An epidemiologic study of altered clinical reactivity to respiratory syncytial (RS) virus infection in children previously vaccinated with an inactivated RS virus vaccine. American Journal of Epidemiology 89, 405–421.CrossRefGoogle ScholarPubMed
Lewis, F. A., Rae, M. L., Lehmann, N. I. & Ferris, A. A. (1961). A syncytial virus associated with epidemic disease of the lower respiratory tract in infants and young children. Medical Journal of Australia 48, 932–933.CrossRefGoogle Scholar
McClelland, A. J. (1980). Vaccination against paramyxoviruses. Nature 284, 404.CrossRefGoogle ScholarPubMed
McNulty, M. S., Bryson, D. G. & Allan, G. M. (1983). Experimental respiratory syncytial virus pneumonia in young calves: Microbiologic and immunofluorescent findings. American Journal of Veterinary Research 44, 1656–1659.Google ScholarPubMed
Mohanty, S. B., Lillie, M. G. & Ingling, A. L. (1976). Effect of serum and nasal neutralizing antibodies on bovine respiratory syncytial virus infection in calves. Journal of Infectious Diseases 134, 409–413.CrossRefGoogle ScholarPubMed
Nuttall, P. A., Luther, P. D.& Stott, E. J. (1977). Viral contamination of bovine foetal serum and cell cultures. Nature 266, 835–837.CrossRefGoogle ScholarPubMed
Nuttall, P. A., Stott, E. J. & Thomas, L. H. (1980). Experimental infection of calves with two strains of bovine virus diarrhoea virus: virus recovery and clinical reactions. Research in Veterinary Science 28, 91–95.CrossRefGoogle ScholarPubMed
Powell, P. C. (1975). Immunity to Mareks disease induced by glutaraldehyde-treated cells of Mareks disease lymphoblastoid cell lines. Nature 257, 684–685.CrossRefGoogle Scholar
Prince, G. A., Potash, L., Horswood, R. L., Camargo, E., Suffin, S. C., Johnson, R. A. & Chanock, R. M. (1979). Intramuscular inoculation of live respiratory syncytial virus induces immunity in cotton rats. Infection and Immunity 23, 723–728.CrossRefGoogle ScholarPubMed
Probert, M.& Russell, S. M. (1975). Measurement of parainfluenza-3 virus antibody by the single radial haemolysis technique. Journal of Clinical Microbiology 2, 157–161.CrossRefGoogle Scholar
Stott, E. J., Bew, M. H., Taylor, G., Jebbett, J. & Collins, A. P. (1984). The characterisation and uses of monoclonal antibodies to respiratory syncytial virus. Developments in Biological Standardisation. (In the Press.)Google Scholar
Stott, E. J., Thomas, L. H., Collins, A. P., Crouch, S., Jebbett, J., Smith, G. S., Luther, P. D. & Caswell, R. (1980). A survey of virus infections of the respiratory tract of cattle and their association with disease. Journal of Hygiene 85, 257–270.CrossRefGoogle ScholarPubMed
Taylor, G. (1979). Solid-phase micro-radioimmunoassay to measure immunoglobulin classspecific antibody to Mycoplasma pulmonis. Infection and Immunity 24, 701–706.CrossRefGoogle ScholarPubMed
Taylor, G., Stott, E. J., Bew, M., Fernie, B. F. & Cote, P. J. (1983). Monoclonal antibodies protect against respiratory syncytial virus. Lancet ii, 976.CrossRefGoogle Scholar
Taylor, G., Stott, E. J., Bew, M., Fernie, B. F., Cote, P. J., Collins, A. P., Hughes, M. & Jebbett, J. (1984). Monoclonal antibodies protect against respiratory syncytial virus infection in mice. Immunology 52, 137–142.Google ScholarPubMed
Thomas, L. H., Stott, E. J., Collins, A. P. & Jebbett, J. (1984). Experimental pneumonia in gnotobiotic calves produced by respiratory syncytial virus. British Journal of Experimental Pathology 65, 19–28.Google ScholarPubMed
Thomas, L. H., Stott, E. J., Collins, A. P., Jebbett, N. J. & Stark, A. J. (1977). Evaluation of respiratory disease in calves: comparison of disease response to different viruses. Research in Veterinary Science 23, 157–164.CrossRefGoogle ScholarPubMed
Tyeryar, F. J. (1983). Report of a workshop on respiratory syncytial virus and parainfluenza viruses. Journal of Infectious Diseases 148, 588–598.CrossRefGoogle ScholarPubMed
Wellemans, G. E., Van Opdenbosch, Ch. V., Boucque, J., Leunen, J. & Strobbe, R. (1978). Vaccination des bovins contre le virus respiratoire syncytial (RSB) au moyen d'une souche atténuee. Annales de Medecine Veterinaire 122, 527–536.Google Scholar
Wright, P. F., Mills, J. & Chanock, R. M. (1971). Evaluation of a temperature-sensitive mutant of respiratory syncytial virus in adults. Journal of Infectious Diseases 124, 505–511.CrossRefGoogle ScholarPubMed
Zaia, J. A. & Oxman, M. N. (1977). Antibody to varicella-zoster virus-induced membrane antigen: immunofluorescence assay using monodisperse glutaraldehyde fixed target cells. Journal of Infectious Diseases 136, 519–530.CrossRefGoogle ScholarPubMed
Zygraich, N.& Wellemans, G. (1981). Immunological markers of an attenuated bovine respiratory syncytial (BRS) virus vaccine. Zentralblatt fur Veterinarmedizin B 28, 355–362.CrossRefGoogle ScholarPubMed