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Comparison of selenite F, Muller-Kauffmann tetrathionate and Rappaport's medium for salmonella isolation from chicken giblets after pre-enrichment in buffered peptone water

Published online by Cambridge University Press:  25 March 2010

R. W. S. Harvey  and
T. H. Price
R. W. S. Harvey
Affiliation:
Regional Public Health Laboratory, University Hospital of Wales, Heath Park, Cardiff CF4 4XW, Wales
T. H. Price
Affiliation:
Regional Public Health Laboratory, University Hospital of Wales, Heath Park, Cardiff CF4 4XW, Wales
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Six hundred and eighty three samples of chicken giblets were examined for salmonellas. Three hundred and forty nine of these were neck and crop specimens and 334 were combined liver and heart samples. Two hundred and ten, in all, contained salmonellas.

The technique of examination included pre-enrichment in buffered peptone water at 37 °C for 18 h and subculture to three enrichment media: Muller-Kauffmann tetrathionate, selenite F and Rappaport's magnesium chloride malachite green broth. Inocula from buffered peptone water to 10 ml of tetrathionate and selenite were 1 ml in each case. The inoculum from the pre-enrichment medium to 10 ml of Rappaport was 0·005 ml. Tetrathionate and selenite were incubated at 43 °C for 48 h. Rappaport's medium was incubated at 37 °C for 48 h. Subcultures from all three enrichment broths were made at 24 h and 48 h to brilliant green MacConkey agar. Selective agars were incubated at 37 °C for 24 h.

The most successful technique for salmonella isolation used Rappaport's medium, which was significantly more efficient than either tetrathionate or selenite. This finding reinforces results obtained using sewage polluted natural water as test material and it is suggested that routine examination of environment samples for salmonellas could be based on Rappaport's medium alone.

If S. typhi, S. dublin or subgenus III salmonellas were likely to be present in the sample, the technique described here would require modification.

Type
Research Article
Information
Journal of Hygiene , Volume 87 , Issue 2 , October 1981 , pp. 219 - 224
Copyright
Copyright © Cambridge University Press 1981

References

Anon (1975). Meat and Meat products – Detection of salmonellae (reference method) International Standard ISO 3565 – 1975 (E). Geneva: International Organization for Standardization.Google Scholar
Chau, P. Y. & Leung, Y. K. (1978). Inhibitory action of various plating media on the growth of certain Salmonella serotypes. Journal of Applied Bacteriology 45, 341.CrossRefGoogle ScholarPubMed
Dixon, J. M. S. (1961). Rapid isolation of salmonellae from faeces. Journal of Clinical Pathology 14, 397.CrossRefGoogle ScholarPubMed
Gould, L. N. & Rhodes, F. (1969). Salmonellae in chicken giblets. The Medical Officer 122, 31.Google Scholar
Harvey, R. W. S. (1956). Choice of a selective medium for the routine isolation of members of the salmonella group. Monthly Bulletin of the Ministry of Health and the Public Health Laboratory Service 15, 118.Google ScholarPubMed
Harvey, R. W. S. & Phillips, W. P. (1955). Survival of Salmonella paratyphi B in sewers: its significance in investigation of paratyphoid outbreaks. Lancet ii, 137.CrossRefGoogle Scholar
Harvey, R. W. S. & Price, T. H. (1974). Isolation of Salmonellas. Public Health Laboratory Service Monograph Series 8, London: HMSO.Google Scholar
Harvey, R. W. S. & Price, T. H. (1975). Studies on the isolation of Salmonella dublin. Journal of Hygiene 74, 369.CrossRefGoogle ScholarPubMed
Harvey, R. W. S. & Price, T. H. (1976). Isolation of salmonellas from sewage polluted river water using selenite F and Muller-Kauffmann tetrathionate. Journal of Hygiene 77, 333.CrossRefGoogle ScholarPubMed
Harvey, R. W. S. & Price, T. H. (1980). Salmonella isolation with Rappaport's medium after pre-enrichment in buffered peptone water using a series of inoculum ratios. Journal of Hygiene 85, 125.CrossRefGoogle ScholarPubMed
Harvey, R. W. S., Price, T. H. & Hall, M. L. M. (1973). Isolations of subgenus III salmonellas (arizonas) in Cardiff 1959–1971. Journal of Hygiene. 71, 481.CrossRefGoogle ScholarPubMed
Harvey, R. W. S., Price, T. H. & Joynson, D. H. M. (1979 b). Salmonella isolation from hospital areas. Journal of Hygiene 83, 461.CrossRefGoogle ScholarPubMed
Harvey, R. W. S., Price, T. H. & Morgan, J. (1977). Salmonella surveillance with reference to pigs – Cardiff abattoir, 1968–1975. Journal of Hygiene 78, 439.CrossRefGoogle ScholarPubMed
Harvey, R. W. S., Price, T. H. & Xirouchaki, E. (1979a). Comparison of selenite F, Muller-Kauffmann tetrathionate and Rappaport's Medium for the isolation of salmonellas from sewage-polluted natural water using a pre-enrichment technique. Journal of Hygiene 83, 451.CrossRefGoogle ScholarPubMed
Hobbs, B. C. & Allison, V. D. (1945). Studies on the isolation of Bact. typhosum and Bact. paratyphosum B. I. Comparison of the selective value of Wilson and Blair (Difco) and desoxycholate-citrate agars over four liquid enrichment media including Selenite F. Monthly Bulletin of the Ministry of Health and the Public Health Laboratory Service 4, 12.Google Scholar
Jameson, J. E. (1963). A note on the isolation of salmonellae, Journal of Applied Bacteriology 26, 112.CrossRefGoogle Scholar
Leifson, E. (1936). New selenite enrichment media for the isolation of typhoid and paratyphoid (salmonella) bacilli. American Journal of Hygiene 24, 423.Google Scholar
Loeffler, F. (1906). Der kulturelle Nachweiss der Typhusbazillen in Faeces, Erde und Wasser mit Hilfe des Malachitgrüns. Deutsche Medizinisch Wochenschrift 32, 289.CrossRefGoogle Scholar
Rappaport, F., Konforti, N. & Navon, B. (1956). A new enrichment medium for certain salmonellae, Journal of Clinical Pathology 9, 261.CrossRefGoogle Scholar
Schothorst, M. van & Leusden, F. M. van (1972). Studies on the isolation of injured salmonellae from foods. Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene I Abt. (Orig. A) 221, 19.Google ScholarPubMed
Vassiliadis, P., Trichopoulos, D., Papadakis, J. & Politi, G. (1970). Salmonella isolation in abattoirs in Greece. Journal of Hygiene 69, 601.Google Scholar