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Comparison of media and methods for counting Clostridium perfringens in poultry meat and further-processed products

Published online by Cambridge University Press:  15 May 2009

B. W. Adams  and
G. C. Mead
B. W. Adams
Affiliation:
Agricultural Research Council Food Research Institute, Coiney Lane, Norwich NR4 7UA
G. C. Mead
Affiliation:
Agricultural Research Council Food Research Institute, Coiney Lane, Norwich NR4 7UA
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Summary

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A Most Probable Number (MPN) method involving Differential Reinforced Clostridial Medium followed by streaking on Willis & Hobbs medium was com pared with direct plating of samples on Tryptose-Suiphite-Cycloserine agar with out egg yolk, and two forms of Oleandomycin-Polymyxin-Suiphadiazine-Per fringens agar, one being prepared from a commercial, dehydrated product.

With skin samples taken from chicken carcasses at different stages of processing, the three direct plating media gave similar counts of Cl. perfringens whereas results obtained with the MPN method were consistently lower.

Although counts of Cl. perfringens from various further processed products were usually < 10/g, the three plating media showed similar specificity for this organism.

All media supported good growth of reference strains of Clostridium perfringens but it was founsi that physiologically similar species, including Cl. absonum, Cl. paraperfringens and Cl. perenne also grew uninhibited in these media and produced colonies identical with those of Cl. perfringens, thus indicating the need for confirmatory tests for Cl. perfringens when examining natural samples.

Type
Research Article
Information
Journal of Hygiene , Volume 84 , Issue 1 , February 1980 , pp. 151 - 158
Copyright
Copyright © Cambridge University Press 1980

References

REFERENCES

Angelotti, R., Hall, H. E., Foter, M. J. & Lewis, K. H. (1962). Quantitation of Clostridium perfringens in foods. Applied Microbiology 10, 193–9.CrossRefGoogle ScholarPubMed
Barnes, E. M., Mead, G. C., Impey, C. S. & Adams, B. W. (1978). Analysis of the avian intestinal flora. In Society for Applied Bacteriology Technical Series no. 11. Techniques for the Study of Mixed Populations (ed. Lovelock, D. W. and Davies, R.), pp. 89–105. London: Academic Press.Google Scholar
Erickson, J. E. & Deibel, R. H. (1978). New medium for rapid screening and enumeration of Clostridium perfringens in foods. Applied and Environmental Microbiology 36, 567–71.CrossRefGoogle ScholarPubMed
Gibbs, P. A. (1971). The incidence of clostridia in poultry carcasses and poultry processing plants. British Poultry Science 12, 101–110.CrossRefGoogle ScholarPubMed
Gibbs, P. A. (1973). The detection of Clostridium welchii in the Differential Reinforced Clostridial Medium technique. Journal of Applied Bacteriology 36, 23–33.CrossRefGoogle ScholarPubMed
Gibbs, B. M. & Freame, B. (1965). Methods for the recovery of clostridia from foods. Journal of Applied Bacteriology 28, 95–111.CrossRefGoogle Scholar
Hagberg, M. M., Busta, F. F., Zottola, E. A. & Arnold, E. A. (1973). Incidence of potentially pathogenic microorganisms in further-processed turkey products. Journal of Milk and Food Technology 36, 625–34.CrossRefGoogle Scholar
Handford, P. M. (1974). A new medium for the detection and enumeration of Clostridium perfringens in foods. Journal of Applied Bacteriology 37, 559–70.CrossRefGoogle ScholarPubMed
Hauschild, A. H. W. & Hilsueimer, R. (1974 a). Evaluation and modifications of media for enumeration of Clostridium perfringens. Applied Microbiology 27, 78–82.CrossRefGoogle ScholarPubMed
Hauschild, A. H. W. & Hilsheimer, R. (1974 b). Enumeration of food-borne Clostridium perfringens in egg yolk-free tryptose-sulphite-cyclosine agar. Applied Microbiology 27, 521–6.CrossRefGoogle ScholarPubMed
Hauschild, A. H. W., Gilbert, R. J., Harmon, S. M., O'kbefe, M. F. & Vaiileteld, R. (1977). ICMSF methods studies. VIII. Comparative study for the enumeration of Clostridium perfringens. Canadian Journal of Microbiology 23, 884–92.CrossRefGoogle ScholarPubMed
Lillard, H. W. (1971). Occurrence of Clostridium perfringens in broiler processing and further processing operations. Journal of Food Science 36, 1008–10.CrossRefGoogle Scholar
Mead, G. C. (1969). Growth and sporulation of Clostridium welchii in breast and leg muscle of poultry. Journal of Applied Bacteriology 32, 86–95.CrossRefGoogle ScholarPubMed
Mead, U. C. & Impey, C. S. (1970). The distribution of clostridia in poultry processing plants. British Poultry Science 11, 407–14.CrossRefGoogle ScholarPubMed
Pinegar, J. A. & Stringer, M. F. (1977). Outbreaks of food poisoning attributed to lecithinase-negative Clostridium welchii. Journal of Clinical Patlwlogy 30, 491–2.CrossRefGoogle ScholarPubMed
Smith, H. W. (1965). The development of the flora of the alimentary tract in young animals. Journal of Pathology and Bacteriology 90, 495–513.CrossRefGoogle ScholarPubMed
Smith, L. Ds. (1970). Factors affecting the growth of Clostridium perfringens. Third International Congress of Food Science and Technology, Washington DC, pp. 661–664.Google Scholar
Trakulcrang, S. P. & Kraft, A. A. (1977). Survival of Clostridium perfringens in refrigerated and frozen meat and poultry items. Journal of Food Science 42, 518–21.CrossRefGoogle Scholar
Vernon, E. (1977). Food poisoning and salmonella infections in England and Wales, 1973–1975. Public Health, London 91, 225–35.CrossRefGoogle Scholar
Willis, A. T. & Hobbs, G. (1959). Some new media for the isolation and identification of clostridia. Journal of Pathology and Bacteriology 77, 511–21.CrossRefGoogle ScholarPubMed
Zottola, E. A. & Busta, F. F. (1971). Microbiological quality of further-processed turkey products. Journal of Food Science 36, 1001–4.CrossRefGoogle Scholar