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A comparison of confirmatory media for coliform organisms and Escherichia coli in water

Published online by Cambridge University Press:  25 March 2010

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Gas production by coliform organisms and Escherichia coli from lauryl tryptose lactose broth (LTLB) was compared with that from brilliant green (lactose) bile broth (BGB). These media were compared with lauryl tryptose mannitol broth (LTMB) with and without added tryptophan for both gas and indole production. At 37 °C, LTLB and BGB were both satisfactory for gas production, but at 44 °C, LTLB gave fewer false-negative results and was thus significantly less inhibitory than BGB. However when LTLB and LTMB were compared as single-tube confirmatory media, LTLB give a high proportion of false-negative reactions in the indole test at 44 °C. The substitution of mannitol for lactose and the addition of tryptophan yielded a satisfactory medium for both confirmation of gas production and the demonstration of indole at 44 °C.

Type
Research Article
Information
Journal of Hygiene , Volume 87 , Issue 3 , December 1981 , pp. 369 - 375
Copyright
Copyright © Cambridge University Press 1981

References

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