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Comparison of agglutination, complement fixation and immunofluorescence tests in Campylobacter jejuni infections

Published online by Cambridge University Press:  25 March 2010

K. C. Watson  and
E. J. C. Kerr
K. C. Watson
Affiliation:
Central Microbiological Laboratories, Western General Hospital, Edinburgh EH4 2XU
E. J. C. Kerr
Affiliation:
Central Microbiological Laboratories, Western General Hospital, Edinburgh EH4 2XU
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Summary

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Good antibody responses usually follow infection with Campylobacter jejuni. A comparison of agglutination, complement fixation and immunofluorescence tests was done on 55 sera from 40 sporadic patients with diarrhoeal disease and positive cultures for C. jejuni. Results showed 82 % positive with immunofluorescence, 62 % by complement fixation but only 38 % by agglutination, using two reference strains COP and MEL as antigens. Overall 90 % of the 40 patients were positive by one or more serological tests. Paired sera from 15 patients showed a fourfold or greater rise in only five, confirming previous observations that antibody formation occurs early in the course of infection. Results also suggest that different test systems may be detecting antibodies of different specificities. Results confirm the value of serological tests, but further information on serotypes is required for selection of suitable reference strains.

Type
Research Article
Information
Journal of Hygiene , Volume 88 , Issue 2 , April 1982 , pp. 165 - 171
Copyright
Copyright © Cambridge University Press 1982

References

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