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Characterization of non-typable strains of Staphylococcus aureus from cases of hospital infection

Published online by Cambridge University Press:  19 October 2009

Ana Vindel ,
Cecilia Martín-Bourgon ,
Juan A. Saez-Nieto  and
Saez Nieto
Ana Vindel
Affiliation:
Laboratorio de Referenda de Fagolipia de S. aureus, Servicio de Bacteríologia, Centro Nacional de Microbiología, Virología e Immunología Sanitarias, 28220 Majadahonda, Madrid, Spain
Cecilia Martín-Bourgon
Affiliation:
Laboratorio de Referenda de Fagolipia de S. aureus, Servicio de Bacteríologia, Centro Nacional de Microbiología, Virología e Immunología Sanitarias, 28220 Majadahonda, Madrid, Spain
Juan A. Saez-Nieto
Affiliation:
Laboratorio de Referenda de Fagolipia de S. aureus, Servicio de Bacteríologia, Centro Nacional de Microbiología, Virología e Immunología Sanitarias, 28220 Majadahonda, Madrid, Spain
Saez Nieto
Affiliation:
Laboratorio de Referenda de Fagolipia de S. aureus, Servicio de Bacteríologia, Centro Nacional de Microbiología, Virología e Immunología Sanitarias, 28220 Majadahonda, Madrid, Spain
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Summary

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A high percentage of non-typable (NT) Staphylococcus aureus strains was isolated in Spanish hospitals during 1984 and 1985. Several alternative methods of typing were employed to study these isolates. These were: phage-typing at 1000 × RTD, phage-typing after heat-treatment (48 °C), thermal shock (56 °C), reverse-typing and induction of additional phages. Using these methods the number of NT isolates was reducedby 60%. Best results were obtained with heat-treatment. Additional phages and reverse-typing were also useful.

A scheme for the study of outbreaks and sporadic cases caused by NT strains is proposed using the methods described.

Type
Research Article
Information
Epidemiology & Infection , Volume 99 , Issue 1 , August 1987 , pp. 191 - 200
Copyright
Copyright © Cambridge University Press 1987

References

REFERENCES

Barry, A. L., Lachica, R. V. F. & Atchison, F. V. (1973). Identification of S. aureus by simultaneous use of the tube coagulase and thermonuclease tests. Applied Microbiology 25, 490–497.CrossRefGoogle ScholarPubMed
Blair, J. E. & Williams, R. E. O. (1961). Phage typing of staphylococci. Bulletin of World Health Organization 24, 771–784.Google ScholarPubMed
DeSaxe, M. J. & Notley, C. M. (1979). Experiences with typing of congulase-negative staphylococci and micrococci. In Phage Typing of Coagulase Negative Staphylococci (ed. Pulverer, G., Hecko, P. B. and Peters, G.), pp. 40–59. Stuttgar, New York: Fisher-Verlag.Google Scholar
Dowset, E. G., Petts, D. N., Baker, S. L., Desaxe, M. J., Cob, A. E., Naidoo, J. & Noijle, W. C. (1984). Analysis of an outbreak of staphylococcal scalded skin syndrome. Journal of Hospital Infection 5, 391–397.CrossRefGoogle Scholar
Espersen, F.Nielsen, P. B., Lund, K., Sylvest, B. & Jensen, K. (1982). Hospital acquired infections in a burns unit caused by an imported strain of S. aureus with unnusual multiresistance. Journal of Hygiene 88, 535–541.CrossRefGoogle Scholar
Lacey, R. W., Keyword, N. & Lincoln, C. (1984). Staphylococci in the UK: a review. Journal of Antimicrobial Chemotherapy 14, sup. D, 19–25.CrossRefGoogle ScholarPubMed
Linneman, C. C. Jr, Mason, M. & Moore, P. (1982). Methicillin resistant S. auretis. Experience in a general hospital over four years. American Journal of Epidemiology 115, 941–950.CrossRefGoogle Scholar
Lorian, V., Zak, O.Suter, J.& Bruecher, C. (1985). Staphylococci 'in vivo' and 'in vitro' Diagnostic Microbiology and Infectious Diseases 3, 433–444.CrossRefGoogle Scholar
Martin-Bourgon, C. (1985a). Marcadores epidemioldgicos en bacterias causantes do infeceidn hospitalaria: con especial referenda: S. aureus y P. aeruginosa. Tesis Doctoral. Facultad do Ciencias Bioldgicas. Universidad Complutense de Madrid, Spain.Google Scholar
Martin-Bourgon, C. (1985 a). Phage typing of S. aureus from cases of bacteriaemia. Journal of Hospital Infection 6, 429–433.CrossRefGoogle Scholar
Martin-Bourgon, C., Berron, S.& Casal, J. (1985). Hospital infection caused by non-typable S. aureus: application of reverse typing. Journal of Hygiene 94, 201–204.CrossRefGoogle ScholarPubMed
Martin-Bourgon, C., Otero, M. R.& Casal, J. (1981). Fagotipia de S. aureus: antilisis de resultados (1978–1980). Immunologika (Spain) 2, 14–23.Google Scholar
Sompolinsky, D., Samra, S., Karakawa, W. W., Vann, W. F., Arbeit, R., Scheneerson, R. S. & Robbins, J. B. (1985). Encapsulation and capsular types in isolates of S. aureus from different sources and relationship to phage-types. Journal of Clinical Microbiology 22, 838–834.CrossRefGoogle ScholarPubMed
Subcommittee on Phage-Typing of Staphylococci (1963). Report. International Journal of Systematic Bacteriology 2, 119–122.Google Scholar
Subcommittee on Phage-Typing of Staphylococci (1975). Report 1970–1974. International Journal of Systematic Bacteriology 25, 241–242.Google Scholar