Hostname: page-component-78c5997874-dh8gc Total loading time: 0 Render date: 2024-11-05T03:40:45.407Z Has data issue: false hasContentIssue false
  • English
  • Français

Arcobacter cryaerophilus and thermophilic campylobacters in a sewage treatment plant in Italy: two secondary treatments compared

Published online by Cambridge University Press:  15 May 2009

S Stampi ,
O Varoli ,
F Zanetti  and
G De Luca
S Stampi
Affiliation:
Institute of Hygiene, Bologna University, Via S. Giacomo 12, Bologna 40126, Italy
O Varoli
Affiliation:
Institute of Microbiology, Bologna University, Via Massarenti 9, Bologna 40126, Italy
F Zanetti
Affiliation:
Institute of Hygiene, Bologna University, Via S. Giacomo 12, Bologna 40126, Italy
G De Luca
Affiliation:
Institute of Hygiene, Bologna University, Via S. Giacomo 12, Bologna 40126, Italy
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Microaerophilic organisms were monitored in sewage effluent undergoing two secondary treatments: air and oxygen-activated sludge. The mean numbers of Arcobacter cryaerophilus and thermophilic campylobacters detected in incoming sewage were 5639/100 ml and 1720/100 ml respectively.

Secondary treatment in air tanks reduced the population of A. cryaerophilus by 97.1% and of thermophilic campylobacters by 99.08%, whereas treatment in oxygen tanks reduced the bacteria 97.8% and 99.63% respectively, showing that oxygen-activated sludge treatment was more effective. Subsequent tertiary treatment with 2 p.p.m. chlorine dioxide evidenced the removal of A. cryaerophilus to 99.9% and eliminated thermophilic campylobacters.

Campylobacter jejuni and C. coli constituted 54.1% and 45.9% of 74 thermophilic campylobacter strains isolated. In air-activated sludge effluent C. jejuni was found more often, thus appearing more sensitive to oxygen.

The most probable number assay used for detection of campylobacters, blood medium for enrichment and blood-free medium for plating, also appeared to be fit for A. cryaerophilus, the high density of which in urban sewage may be due to inflows from slaughterhouses.

Type
Research Article
Information
Epidemiology & Infection , Volume 110 , Issue 3 , June 1993 , pp. 633 - 639
Copyright
Copyright © Cambridge University Press 1993

References

REFERENCES

1.Vandamme, P. Falsen, E. Rossau, R, et al. Revision of Campylobacter. Helicobacter. and Wolinella taxonomy: emendation of generic description and proposal of Arcobacter gen. nov. Int J System Bacteriol 1991: 41: 88–103.CrossRefGoogle ScholarPubMed
2.Goodwin, CS. Armstrong, JA. Chilvers, T, et al. Transfer of Campylobacter pylori and Campylobacter mustelae to Helicobacter gen. nov. as Helicobacter pylori comb. nov. and Helicobacter mustelae comb. nov. respectively. Int J System Bacteriol 1989; 39: 397–405.Google Scholar
3.Fennell, CL. Totten, PA. Quinn, TC, Patton, DL, Holmes, KK, Stamm, WE. Characterization of campylobacter-like organisms isolated from homosexual men. J Infect Dis 1984; 149: 58–66.Google Scholar
4.Totten, PA. Tenover, FC. Wezenberg, JM. Perine, PL. Stamm, WE. King, KH. Campylobacter cinaedi (sp. nov.) and Campylobacter fenneliae (sp. nov.): Two new Campylobacter species associated with enteric disease in homosexual men. J Infect Dis 1985; 151: 131–9.CrossRefGoogle Scholar
5.Megraud, F. Bonnet, F. Unusual campylobacters in human faeces. J Infect 1986; 12: 275–6.Google Scholar
6.Tee, W. Anderson, BN. Ross, BC. Dwyer, B. Atypical campylobacters associated with gastroenteritis. J Clin Microbiol 1987; 25: 1248–52.Google Scholar
7.Blaser, MJ. Glass, RI. Hug, MI. Stoll, B, Kibryia, GM, Alim, Arma. Isolation of C. fetus susp. jejuni from Bangladesh children. J Clin Microbiol 1980: 12: 744–7.Google Scholar
8.Mentzing, Lo. Waterborne outbreak of Campylobacter enteritis in central Sweden. Lancet 1981: ii: 352–4.Google Scholar
9.Skirrow, MB. Campylobacter enteritis – the first five years. J Hyg 1982; 89: 175–84.Google Scholar
10.Skirrow, MB. A demographic survey of campylobacter, salmonella and shigella infections in England. Epidemiol Infect 1987; 99: 647–57.Google Scholar
11.Skirrow, MB. Campylobacter. Lancet 1991; i: 213–16.Google Scholar
12.Cabrita, J. Pires, I. Vlaes, L, et al. Campylobacter enteritis in Portugal: Epidemiological features and biological markers. European J Epidemiol 1992: 8: 22–6.CrossRefGoogle ScholarPubMed
13.Kiehlbauch, JA. Brenner, DJ. Nicholson, MA. Baker, CN. Steigerwalt, AG. Wachsmuth, IK. Campylobacter butzleri (sp. nov.) isolated from humans and animals with diarrheal disease. J Clin Microbiol 1991: 29: 376–85.Google Scholar
14.Taylor, DN. Kiehlbauch, JA. Tee, W. Pitarangsi, C. Echeverria, P. Isolation of Group 2 Campylobacter species from Thai children with diarrhea. J Infect Dis 1991: 163: 1062–7.CrossRefGoogle ScholarPubMed
15.Ellis, WA. Neill, SD. O’Brien, JJ. Ferguson, HW. Hanna, J. Isolation of Spirillum/Vibrio-like organisms from bovine fetuses. Vet Rec 1977: 100: 451–2.Google Scholar
16.Ellis, WA. Neill, SD. O’Brien, JJ. Hanna, J. Isolation of Spirillum-like organisms from pig fetuses. Vet Rec 1978: 102: 106.Google Scholar
17.Neill, SD. Ellis, WA. O’Brien, JJ. The biochemical characteristics of Campylobacter-like organisms from cattle and pigs. Res Vet Sci 1978: 25: 368–72.Google Scholar
18.Neill, SD. Ellis, WA. O’Brien, JJ. Designation of aerotolerant Campylobacter-like organisms from porcine and bovine abortions to the genus campylobacter. Res Vet Sci 1979; 27: 1806.Google Scholar
19.Boudreau, M. Higgins, R. Mittal, KR. Biochemical and serological characterization of Campylobacter cryaerophila. J Clin Microbiol 1991: 29: 54–8.Google Scholar
20.Tee, W. Baird, R. Dyall-Smith, M, Dwyer, B. Campylobacter cryaerophila isolated from a human. J Clin Microbiol 1988; 26: 2469–73.Google Scholar
21.Stampi, S. Varoli, O. Zanetti, F. De Luca, G. Indagine sulla presenza di Campylobacter in acque superficiali. Ig San Pubbl 1991: 47: 132–45.Google Scholar
22.Stampi, S. Varoli, O. Zanetti, F. De Luca, G. Occurrence. removal and seasonal variation of thermophilic campylobacters in a sewage treatment plant in Italy. Zbl Hyg 1992; 193: 199–210.Google Scholar
23.Lior, H. New. extended biotyping scheme for Campylobacter jejuni, Campylobacter coli and ‘Campylobacter laridis’. J Clin Microbiol 1984; 20: 636–40.CrossRefGoogle ScholarPubMed
24.Penner, JL. The genus Campylobacter: a decade of progress. Clin Microbiol Rev 1988; 3: 157–72.Google Scholar