Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-17T14:58:29.176Z Has data issue: false hasContentIssue false
  • English
  • Français

An analysis of the diversity of Haemophilus parainfluenzae in the adult human respiratory tract by genomic DNA fingerprinting

Published online by Cambridge University Press:  15 May 2009

G. R. D. Kerr ,
K. J. Forbes ,
A. Williams  and
T. H. Pennington
G. R. D. Kerr
Affiliation:
British Antarctic Survey Medical Unit, Robert Gordons Institute of Technology Survival Centre, King Street, Aberdeen AB2 3BJ
K. J. Forbes*
Affiliation:
Department of Medical Microbiology, University of Aberdeen, Foresterhill, Aberdeen AB9 2ZD
A. Williams
Affiliation:
Department of Medical Microbiology, University of Aberdeen, Foresterhill, Aberdeen AB9 2ZD
T. H. Pennington
Affiliation:
Department of Medical Microbiology, University of Aberdeen, Foresterhill, Aberdeen AB9 2ZD
*
* Author for correspondence.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A method for typing Haemophilus species is described, based on the analysis of genomic DNA from Haemophilus parainfluenzae. The DNA was extracted by a rapid method and digested with the restriction enzyme BamHI to provide a characteristic ‘fingerprint’. The pattern of fragments in the ranges 1–1·6 kb, 1·6–2 kb and 2–3 kb were used to produce a numerical profile of each isolate. In total 97 isolates were examined; 88 from throat swab material isolated from the 15 members of a British Antarctic Survey base and 9 type strains. Seventy-two of the 88 antarctic isolates were H. parainfluenzae and were found to be very diverse, comprising 41 identifiable strains with up to 5 strains being isolated from a single throat swab sample. There was evidence for both carriage and transmission within the isolated community. The technique provided a highly discriminatory method for characterizing Haemophilus strains which is suitable for epidemiological studies.

Type
Research Article
Information
Epidemiology & Infection , Volume 111 , Issue 1 , August 1993 , pp. 89 - 98
Copyright
Copyright © Cambridge University Press 1993

References

1.Palmer, GG. Haemophilus in faeces. J Med Microbiol 1981; 14: 147–50.CrossRefGoogle ScholarPubMed
2.Kilian, M. A taxonomic study of the genus Haemophilus with the proposal of a new species. J Gen Microbiol 1976; 93: 9–62.CrossRefGoogle ScholarPubMed
3.Kilian, M, Biberstein, EL. Genus II. Haemophilus. In: Krieg, NR, Holt, JH, eds. Bergey's manual of systematic bacteriology. Vol I. Baltimore: Williams and Wilkins, 1984: 558–69.Google Scholar
4.Kilian, M, Heine-Jensen, J, Bülow, P. Haemophilus in the upper respiratory tract of children. Acta Path Microbiol Scand 1972; 80: 571–8.Google ScholarPubMed
5.Kuklinska, D, Kilian, M. Relative proportions of Haemophilus species in the throat of healthy children and adults. Eur J Clin Microbiol 1984; 3: 249–52.CrossRefGoogle ScholarPubMed
6.Sims, W. Oral haemophili. J Med Microbiol 1970; 3: 615–25.CrossRefGoogle ScholarPubMed
7.Kilian, M, Schiøtt, CR. Haemophili and related bacteria in the human oral cavity. Archs Oral Biol 1975; 20: 791–6.CrossRefGoogle ScholarPubMed
8.Albritton, WL. Infections due to Haemophilus species other than H. influenzae. Ann Rev Microbiol 1982; 36: 199–216.CrossRefGoogle ScholarPubMed
9.Turk, DC, May, JR. H. influenzae. Its clinical importance. London: English University Press, 1967: 11.Google Scholar
10.Rhind, GB, Gould, GA, Ahmad, F, Croughan, MJ, Calder, MA. Haemophilus parainfluenzae and H. influenzae respiratory infection: comparison of clinical features. BMJ 1985; 291: 707–8CrossRefGoogle Scholar
11.Weinberg, GA, Ghafoor, A, Ishaq, Z et al. Clonal analysis of Haemophilus influenzae isolated from children from Pakistan with lower respiratory tract infections. J Infect Dis 1989; 160: 634–43.CrossRefGoogle ScholarPubMed
12.Porras, O, Caugant, DA, Gray, B, Lagergård, T, Levin, BR, Svanborg-Edén, C. Difference in structure between type b and nontypable Haemophilus influenzae populations. Infect Immun 1986; 53: 79–89.CrossRefGoogle ScholarPubMed
13.Musser, JM, Kroll, JS, Granoff, DM et al. Global genetic structure and molecular epidemiology of encapsulated Haemophilus influenzae. Rev Infect Dis 1990; 12: 75–111.CrossRefGoogle ScholarPubMed
14.Pennington, TH. Haemophilus species and clones. Rev Med Microbiol 1993; 4: In press.CrossRefGoogle Scholar
15.Loos, BG, Bernstein, JM, Dryja, DM, Murphy, TF, Dickinson, DP. Determination of the epidemiology and transmission of nontypeable Haemophilus influenzae in children with otitis media by comparison of total genomic DNA restriction fingerprints. Infect Immun 1989; 57: 2751–7.CrossRefGoogle Scholar
16.Groeneveld, K, Van Alphen, L, Eijk, PP, Visschers, G, Jansen, HM, Zanen, HC. Endogenous and exogenous reinfections by Haemophilus influenzae in patients with chronic obstructive pulmonary disease: the effect of antibiotic treatment on persistence. J Infect Dis 1990; 161: 512–7.CrossRefGoogle ScholarPubMed
17.Doern, GV, Chapin, KC. Determination of biotypes of Haemophilus influenzae and Haemophilus parainfluenzae; a comparison of methods and description of a new biotype (VIII) of H. parainfluenzae. Diagn Microbiol Infect Dis 1987; 7: 269–72.CrossRefGoogle ScholarPubMed
18.Watson, KC, Kerr, EJC, Baillie, M. Comparison of API-10S and tube tests for biotyping H. influenzae. J Clin Pathol 1986; 39: 692–693CrossRefGoogle Scholar
19.Forbes, KJ, Bruce, KD, Jordens, JZ, Ball, A, Pennington, TH. Rapid methods in bacterial DNA fingerprinting. J Gen Microbiol 1991; 137: 2051–8.CrossRefGoogle ScholarPubMed
20.Sambrook, J, Fritsch, EF, Maniatis, T. Molecular cloning; a laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 1989.Google Scholar
21.Roberts, MC, Mintz, CS, Morse, SA. Characterisation of Haemophilus parainfluenzae strains with low-Mr or ladder-like lipopolysaccharides. J Gen Microbiol 1986; 132: 611–6.Google ScholarPubMed
22.Roberts, MC, Bell, TA, Sandstrom, KI, Smith, AL, Holmes, KK. Characterisation of Haemophilus spp isolated from infant conjunctivities. J Med Microbiol 1986; 21: 219–24.CrossRefGoogle Scholar
23.Bruce, KD, Jordens, JZ. Characterisation of noncapsulate Haemophilus influenzae by whole cell polypeptide profiles, restriction endonuclease analysis, and rRNA gene restriction patterns. J. Clin Microbiol 1991; 29: 291–6.CrossRefGoogle ScholarPubMed
24.Hedges, AJ, Howe, K, Linton, AH. Statistical considerations in the sampling of Escherichia coli from intestinal sources for serotyping. J Appl Bacteriol 1977; 43: 271–80.CrossRefGoogle ScholarPubMed
25.Caugant, DA, Levin, BR, Selander, RK. Genetic diversity and temporal variation in the E. coli population of a human host. Genetics 1981; 98: 467–90.CrossRefGoogle Scholar
26.Maggs, AF, Pennington, TH. Temporal study of Staphylococcal species on the skin of human subjects in isolation and clonal analysis of Staphylococcus capitis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. J Clin Microbiol 1989; 27: 2627–32.CrossRefGoogle ScholarPubMed
27.Tzabar, Y, Pennington, TH. The population structure and transmission of E. coli in an isolated human community; studies on an Antarctic base. Epidemiol Infect 1991; 107: 537–42.CrossRefGoogle Scholar
28.Caugant, DA, Levin, BR, Selander, RK. Distribution of multilocus genotypes of Escherichia coli within and between host families. J Hyg 1984; 92: 377–84.CrossRefGoogle ScholarPubMed
29.Bruce, KD, Pennington, TH. Clonal analysis of non-typable Haemophilus influenzae by sodium dodecyl sulphate-polyacrylamide gel electrophoresis of whole cell polypeptides. J Med Microbiol 1991; 34: 277–83.CrossRefGoogle ScholarPubMed
30.Pohl, S. DNA relatedness among members of Haemophilus, Pasteurella and Actinobacillus. In: Kilian, M, Frederiksen, W, Biberstein, EL, eds. Haemophilus, Pasteurella and Actinobacillus. London: Academic Press. 1981: 245–53.Google Scholar
31.Dewhirst, FE, Paster, BJ, Olsen, I, Frazer, GJ. Phylogeny of 54 representative strains of species in the family Pasteurellaceae as determined by comparison of 16S rRNA sequences. J Bacteriol 1992; 17: 2002–13.CrossRefGoogle Scholar