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Aminoadamantane-resistant strains of influenza A2 virus

Published online by Cambridge University Press:  15 May 2009

J. S. Oxford  and
C. W. Potter
J. S. Oxford
Affiliation:
Department of Microbiology, John Curtin School of Medical Research, Australian National University, Canberra, A.C.T. Australia and Department of Medical Microbiology, The University, Sheffield, England
C. W. Potter
Affiliation:
Department of Microbiology, John Curtin School of Medical Research, Australian National University, Canberra, A.C.T. Australia and Department of Medical Microbiology, The University, Sheffield, England
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After one passage of influenza A2/Singapore/l/57 virus in mice treated with 150 mg./kg./day of aminoadamantane, a partially drug-resistant strain of virus was detected in 1 of 12 mice. The isolation rate of aminoadamantane-resistant viruses increased to 8 after three passages in drug-treated mice. Some virus strains showed a 500-fold increase in resistance to aminoadamantane and to the structurally related compounds α-methyl-1-adamantane methylamine and 2-adamantanamine sulphate. No aminoadamantane-resistant viruses were detected after passage of influenza four times in mice treated with lower (15 or 1·5 mg./kg./day) concentrations of aminoadamantane. Aminoadamantane had no detectable effect on the development of lung lesions in mice infected with the drug-resistant influenza strain, whereas lung lesions were reduced in aminoadamantane treated mice infected with a control strain of influenza A2/Singapore virus. No differences were detected in the buoyant density in caesium chloride, morphology or serology between control and aminoadamantane-resistant strains of virus. These drug-resistant influenza viruses may be useful for detailed studies of the mode of action of aminoadamantane.

Type
Research Article
Information
Journal of Hygiene , Volume 71 , Issue 2 , June 1973 , pp. 227 - 236
Copyright
Copyright © Cambridge University Press 1973

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