Hostname: page-component-78c5997874-94fs2 Total loading time: 0 Render date: 2024-11-19T20:43:47.037Z Has data issue: false hasContentIssue false
  • English
  • Français

Hybridization rates between lettuce (Lactuca sativa) andits wild relative (L. serriola) under field conditions

Published online by Cambridge University Press:  30 May 2008

Luigi D'Andrea ,
François Felber  and
Roberto Guadagnuolo
Luigi D'Andrea
Affiliation:
Laboratoire de botanique évolutive, Institut de biologie, Université de Neuchâtel, Rue Émile-Argand 11, CP 158, 2009 Neuchâtel, Switzerland
François Felber
Affiliation:
Laboratoire de botanique évolutive, Institut de biologie, Université de Neuchâtel, Rue Émile-Argand 11, CP 158, 2009 Neuchâtel, Switzerland
Roberto Guadagnuolo
Affiliation:
Laboratoire de botanique évolutive, Institut de biologie, Université de Neuchâtel, Rue Émile-Argand 11, CP 158, 2009 Neuchâtel, Switzerland

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Hybridization and introgression between crops and wild relatives may have important evolutionary and ecological consequences such as gene swamping or increased invasiveness. In the present study, we investigated hybridization under field conditions between crop lettuce (Lactuca sativa) and its wild relative prickly lettuce (L. serriola), two cross-compatible, predominantly autogamous and insect pollinated species. In 2003 and 2004, we estimated the rates of hybridization between L. sativa and L. serriola in close-to-reality field experiments carried out in two locations of Northern Switzerland. Seeds set by the experimental wild plants were collected and sown (44 352 in 2003 and 252 345 in 2004). Progeny was screened morphologically for detecting natural hybrids. Prior to the experiment, specific RAPD markers were used to confirm that morphological characters were reliable for hybrid identification. Hybridization occurred up to the maximal distance tested (40 m), and hybridization rates varied between 0 to 26%, decreasing with distance. More than 80% of the wild plants produced at least one hybrid (incidence of hybridization, IH) at 0 m and 1 m. It equaled 4 to 5% at 40 m. In sympatric crop-wild populations, cross-pollination between cultivated lettuce and its wild relative has to be seen as the rule rather than the exception for short distances.

Keywords

autogamyhybridizationintrogressionLactuca sativaLactuca serriolapollen flowRAPD
Type
Research Article
Information
Environmental Biosafety Research , Volume 7 , Issue 2 , April 2008 , pp. 61 - 71
Copyright
© ISBR, EDP Sciences, 2008

References

Bateman AJ (1947) Contamination in Seed Crops. 3. Relation with isolation distance. Heredity 1: 303–336
Chen, LJ, Lee, DS, Song, ZP, Suh, HS, Lu, BR (2004) Gene flow from cultivated rice (Oryza sativa) to its weedy and wild relatives. Ann. Bot. 93: 67–73. CrossRef
Curtis, IS, He, C, Jordi, W, Davelaar, E, Power, JB, De Laat, AMM, Davey, MR (1999) Promoter deletions are essential for transformation of lettuce by the T-cyt gene: The phenotypes of transgenic plants. Ann. Bot. 83: 559–567 CrossRef
De Vries, IM (1990) Crossing experiments of lettuce cultivars and species (Lactuca sect. Lactuca, Compositae). Pl. Syst. Evol. 171: 233–248 CrossRef
Durst CE (1930) Inheritance in lettuce. Illinois Agr. Exp. Sta. Bull. 356: 237–341
Eenink, AH (1983) Preliminary-results of research on storage and in vitro germination of lettuce pollen as an aid in lettuce breeding. Euphytica 32: 521–526 CrossRef
Ellstrand NC (2003) Dangerous liaisons? When cultivated plants mate with their wild relatives. Baltimore (MD): Johns Hopkins University Press
Frietema de Vries, FT (1992) The systematic relationship of Lactuca sativa and Lactuca serriola, in relation to the distribution of Prickly lettuce. Acta Bot. Neerl. 43: 79
Frietema de Vries, FT, van der Meijden, R, Brandeburg, WA (1994) Botanical files on lettuce: on the chance for gene flow between wild and cultivated Lettuce (L. sativa L. including L. serriola L., Compositae) and the generalised implication for risks assessment on genetically modified plants. Gorteria supplement 2: 44 p
Goto, F, Yoshihara, T, Saiki, H (2000) Iron accumulation and enhanced growth in transgenic lettuce plants expressing the iron-binding protein ferritin. Theor. Appl. Genet. 100: 658–664 CrossRef
Goubara, M, Takasaki, T (2003) Flower visitors of lettuce under field and enclosure conditions. Appl. Entomol. Zool. 38: 571–581 CrossRef
Goubara, M, Takasaki, T (2004) Pollination effects of the sweat bee Lasioglossum villosulum trichopse (Hymenoptera: Halictidae) on genic male-sterile lettuce. Appl. Entomol. Zool. 39: 163–169 CrossRef
Guadagnuolo, R, Clegg, J, Ellstrand, NC (2006) Relative fitness of transgenic vs. non-transgenic maize $\times$ teosinte hybrids: A field evaluation. Ecol. Appl. 16: 1967–1974 CrossRef
Hooftman, DAP, Oostermeijer, JGB, Jacobs, MMJ, den Nijs, HCM (2005) Demographic vital rates determine the performance advantage of crop-wild hybrids in lettuce. J. Appl. Ecol. 42: 1086–1095 CrossRef
Hooftman DAP, Jong MJD, Oostermeijer JGB, den Nijs H (2007) Modelling the long-term consequences of crop-wild relative hybridization: a case study using four generations of hybrids. J. Appl. Ecol. 44: 1035–1045
Ibarra Perez, FJ, Ehdaie, B, Waines, JG (1997) Estimation of outcrossing rate in common bean. Crop Sci. 37: 60–65 CrossRef
Jones, HA (1927) Pollination and life history studies of lettuce (Lactuca sativa L.). Hilgardia 2: 425–479 CrossRef
Klinger, T, Elam, DR, Ellstrand, NC (1991) Radish as a model system for the study of engineered gene escape rates via crop-weed mating. Conserv. Biol. 5: 531–535 CrossRef
Klinger, T, Arriola, PE, Ellstrand, NC (1992) Crop-weed hybridization in radish (Raphanus sativus): effects of distance and population size. Am. J. Bot. 79: 1431–1435 CrossRef
Koopman, WJM, Zevenbergen, MJ, van den Berg, RG (2001) Species relationships in Lactuca s.l. (Lactuceae, Asteraceae) inferred from AFLP fingerprints. Am. J. Bot. 88: 1881–1887 CrossRef
Ladizinsky, G (1984) Founder effect in crop-plant evolution. Econ. Bot. 39: 191–199 CrossRef
Lebeda, A, Dolezalovà, I, Kristkovà, E, Mieslerovà, B (2001) Biodiversity and ecogeography of wild Lactuca ssp. in some European countries. Genet. Resour. Crop Evol. 48: 153–164 CrossRef
Lebeda, A, Dolezalova, I, Ferakova, V, Astley, D (2004) Geographical distribution of wild Lactuca species (Asteraceae, Lactuceae). Bot. Rev. 70: 328–356 CrossRef
Lindqvist, K (1960a) Cytogenetic studies in the serriola group of Lactuca. Hereditas 46: 75–151 CrossRef
Lindqvist, K (1960c) On the origin of cultivated lettuce. Hereditas 46: 319–350 CrossRef
McCabe, MS, Schepers, F, van der Arend, A, Mohapatra, U, de Laat, AMM, Power, JB, Davey, MR (1999) Increased stable inheritance of herbicide resistance in transgenic lettuce carrying a petE promoter-bar gene compared with a CaMV 35S-bar gene. Theor. Appl. Genet. 99: 587–592 CrossRef
Mejias, JA (1994) Self-fertility and associated flower head traits in the Iberian taxa of Lactuca and related genera (Asteraceae, Lactuceae). Plant Syst. Evol. 191: 147–160 CrossRef
Nagata, RT, Dusky, JA, Ferl, RJ, Torres, AC, Cantliffe, DJ (2000) Evaluation of glyphosate resistance in transgenic lettuce. J. Am. Soc. Hoartic. Sci. 125: 669–672
Nakamaya, Y, Yamaguchi, H (2002) Natural hybridization in wild soybean (Glycine max ssp. soja) by pollen flow from cultivated soybean (Glycine max ssp. max) in a designed population. Weed. Biol. Manage. 2: 25–30 CrossRef
Okubara, PA, Arroyo-Garcia, R, Shen, KA, Mazier, M, Meyers, BC, Ochoa, OE, Kim, S, Yang, CH, Michelmore, RW (1997) A transgenic mutant of Lactuca sativa (lettuce) with a T-DNA tightly linked to loss of downy mildew resistance. Mol. Plant Microbe In. 10: 970–977 CrossRef
Ownbey M, Andersson IE (1949) Introgressive Hybridization. New York, London
Pammel, LH (1918) Prickly lettuce. Proc. Ia. Acad Sci. 20: 109
Paul, EM, Capiau, K, Jacobs, M, Dunwell, JM (1995) A study of gene dispersal via pollen in Nicotiana tabacum using introduced genetic markers. J. Appl. Ecol. 32: 875–882 CrossRef
Pileggi, M, Pereiara, AAM, Silva, JD, Pileggi, SAV, Verma, DPS (2001) An improved method for transformation of lettuce by Agrobacterium tumefaciens with a gene that confers freezing resistance. Braz. Arch. Biol. Tech. 44: 191–196 CrossRef
Prince, SD, Marks, MK, Carter, RN (1978) Induction of flowering in wild lettuce (Lactuca serriola L.). New Phytologist 81: 265–277 CrossRef
Rice, WR (1989) Analyzing tables of statistical tests. Evolution 43: 223–225 CrossRef
Schmitt, J (1983) Density-dependent pollinator foraging, flowering phenology, and temporal pollen dispersal patterns in linanthus-bicolor. Evolution 37: 1247–1257 CrossRef
Snow, AA, Palma, PM (1997) Commercialization of transgenic plants: Potential ecological risks. Bioscience 47: 86–96 CrossRef
Snow, AA, Spira, TP (1996) Pollen-tube competition and male fitness in Hibiscus moscheutos. Evolution 50: 1866–1870 CrossRef
Song, ZP, Lu, BR, Zhu, YG, Chen, JK (2003) Gene flow from cultivated rice to the wild species Oryza rufipogon under experimental field conditions. New Phytol. 157: 657–665 CrossRef
Thompson, RC, Whitaker, TW, Bohn, GW (1958) Natural cross-pollination in lettuce. Proc. Am. Soc. Hort. Sci. 36: 403–409
Van Deynze, AE, Sundstrom, FJ, Bradford, KJ (2005) Pollen-mediated gene flow in California cotton depends on pollinator activity. Crop Sci. 45: 1565–1570 CrossRef
Wang, TY, Chen, HB, Reboud, X, Darmency, H (1997) Pollen-mediated gene flow in an autogamous crop: Foxtail millet (Setaria italica). Plant Breed. 116: 579–583 CrossRef
Watts, LE (1958) Natural cross-pollination in lettuce. Nature 181: 1084 CrossRef
Weaver, SE, Downs, MP (2003) The biology of Canadian weeds. 122. Lactuca serriola L. Can. J. Plant Sci. 83: 619–628 CrossRef
Whitaker, TW (1939) Cytogenetic observations in Lactuca. J. Agri. Res. 58: 297–306
Wolfenbarger, LL, Phifer, PR (2000) Biotechnology and ecology - The ecological risks and benefits of genetically engineered plants. Science 290: 2088–2093 CrossRef
Zhang, NY, Linscombe, S, Oard, J (2003) Out-crossing frequency and genetic analysis of hybrids between transgenic glufosinate herbicide-resistant rice and the weed, red rice. Euphytica 130: 35–45 CrossRef