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Facilitating co-existence by tracking gene dispersal in conventional potato systems with microsatellite markers

Published online by Cambridge University Press:  28 November 2007

Carloalberto Petti ,
Conor Meade ,
Martin Downes  and
Ewen Mullins
Carloalberto Petti
Affiliation:
Plant Biotechnology Unit, Teagasc Crops Research Centre, Oak Park, Co. Carlow, Ireland
Conor Meade
Affiliation:
Institute of Bioengineering and Agroecology, National University of Ireland, Maynooth, Co. Kildare, Ireland
Martin Downes
Affiliation:
Institute of Bioengineering and Agroecology, National University of Ireland, Maynooth, Co. Kildare, Ireland
Ewen Mullins
Affiliation:
Plant Biotechnology Unit, Teagasc Crops Research Centre, Oak Park, Co. Carlow, Ireland

Abstract

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Based on international findings, Irish co-existence guidelines for the cultivation of GM potato stipulate that an isolation distance of 20 m is required to minimize the spread of transgenic pollen in accordance with required labeling thresholds. As potato tolerant to Phytophthora infestans is the most applicable GM crop from an Irish context, we tested the efficacy of this isolation distance under Irish environmental conditions using the conventional variety Désirée as a pollen donor and the male-sterile variety British Queen as a pollen receptor. Gene flow was determined by scoring for berry presence on receptor plants and confirmed using a microsatellite marker system designed to assess paternity in F1 seedlings. 99.1% of seedlings recovered were identified as having Désirée paternity. Whereas 19.9% (140/708) of total berries formed on receptor plants occurred at a distance of 21 m from the pollen source, only 4 of these berries bore viable true potato seed (TPS), from which 23 TPS germinated. TPS-bearing berry formation was negatively correlated with distance from the pollen source, and although overall distribution of berries and seeds was non-random across the plot, no significant correlation was evident with respect to wind direction. Microsatellite markers were also used to confirm that the foraging beetle Meligethes aeneus is a vector for the transmission of potato pollen, but a more detailed statistical analysis of this dataset was limited by inclement weather during the trial. To conclude, we recommend that a two-tiered system be established in regard to establishing isolation distances for the experimental trial and commercial cultivation of GM potato in Ireland, and that responsible crop management be adopted to minimize the establishment of TPS-derived volunteers, which we have noted will emerge through a rotation as a result of pollen-mediated gene flow.

Keywords

Solanum tuberosumpotatomicrosatellitegene flowco-existence
Type
Research Article
Information
Environmental Biosafety Research , Volume 6 , Issue 4 , October 2007 , pp. 223 - 235
Copyright
© ISBR, EDP Sciences, 2007

References

ACRE (2004) Advice on scientific issues concerning the proposed regime for the co-existence of GM and non-GM crops, p 6. U.K. Advisory Committee on Releases to the Environment, Department for Environment, Food and Rural Affairs
Andersen, NS, Siegismund, HR, Meyer, V, Jorgensen, RB (2005) Low level of gene flow from cultivated beets (Beta vulgaris L. ssp. vulgaris) into Danish populations of sea beet (Beta vulgaris L. ssp. maritima (L.) Arcangeli). Mol. Ecol. 14: 1391–1405 CrossRef
Celis, C, Scurrah, M, Cowgill, S, Chumbiauca, S, Green, J, Franco, J, Main, G, Kiezebrink, D, Visser, RGF, Atkinson, HJ (2004) Environmental biosafety and transgenic potato in a centre of diversity for this crop. Nature 432: 222–225 CrossRef
Chen, LJ, Lee, DS, Song, ZP, Suh, HS, Lu, B-R (2004) Gene flow from cultivated rice (Oryza sativa) to its weedy and wild relatives. Ann. Bot. 93: 67–73 CrossRef
Conner AJ (1993) Monitoring “escapes” from field trials of transgenic potatoes: a basis for assessing environmental risks. In Seminar on Scientific Approaches for the Assessment of Research Trials with Genetically Modified Plants, OECD, Paris, pp 34–40
Conner, AJ, Dale, PJ (1996) Reconsideration of pollen dispersal data form field trials of transgenic potatoes. Theor. Appl. Genet. 92: 505–508 CrossRef
Desplanque, B, Boudry, P, Broomberg, K, Saumitou- Laprade, P, Cuguen, J, Van Dijk H (1999) Genetic diversity and gene flow between wild, cultivated and weedy forms of Beta vulgaris L. (Chenopodiaceae), assessed by RFLP and microsatellite markers. Theor. Appl. Genet. 98: 1194–1201 CrossRef
Eastham K, Sweet J (2002) Genetically modified organisms (GMOs): The significance of gene flow through pollen transfer, European Environment Agency, Copenhagen, pp 1–75
Fernandez-Cornejo, J, Daberkow, S, McBride, WD (2001) Decomposing the size effect on the adoption of innovations: Agrobiotechnology and precision agriculture. AgBioForum 4: 124–136, http://www.agbioforum.org
Flannery, M-L, Meade, C, Mullins, E (2005) Employing a composite gene-flow index to numerically quantify a crop's potential for gene flow: an Irish perspective. Environ. Biosafety Res. 4: 29–43. CrossRef
Ghislain, M, Spooner, DM, Rodriguez, F, Villamon, F, Nunez, J, Waugh, R, Bonierbale, M (2004) Selection of highly informative and user-friendly microsatellites (SSRs) for genotyping of cultivated potato. Theor. Appl. Genet. 108: 881–890 CrossRef
Lawson, HM (1983) True potato seed as arable weeds. Potato Res. 26: 303–306 CrossRef
McGill NP, Lohan G, O'Reilly B, Thorp C, Armstrong K, Gaule S, Macken F, Downey J, Dardis J, Devlin R, Leech B, McGloughlin T, Mullins E (2005) Coexistence of GM and non-GM Crops in Ireland. Report of the Working Group on the Coexistence of GM crops with Conventional and Organic Farming, Department of Agriculture and Food, Dublin, p 286
McHughen, A (2006) Editorial – The limited value of measuring gene flow via errant pollen from GM plants. Environ. Biosafety Res. 5: 1–2 CrossRef
McPartlan, HC, Dale, PJ (1994) An assessment of gene transfer by pollen from field grown transgenic potatoes to non-transgenic potatoes and related species. Transgenic Res. 3: 216–225 CrossRef
Milbourne, D, Meyer, R, Collins, A, Ramsay, L, Gebhardt, C, Waugh, R (1998) Isolation, characterisation and mapping of simple sequence repeat loci in potato. Mol. Gen. Genet. 259: 233–245 CrossRef
Murashige, T, Skoog, F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497 CrossRef
Ryan, E, Meade, C, Mullins, E, Burke, JI, Downes, M (2006) Tracing field hybridisation in Ryegrass species using microsatellite and morphological markers. Environ. Biosafety Res. 5: 111–117 CrossRef
Skogsmyr, I (1994) Gene dispersal from transgenic potatoes to conspecifics: A field trial. Theor. Appl. Genet. 88: 770–774 CrossRef
Slavov, GT, Howe, GT, Gyaourova, AV, Birkes, DS, Adams, WT (2005) Estimating pollen flow using SSR markers and paternity exclusion: accounting for mistyping. Mol. Ecol. 14: 3109–3121 CrossRef
Tolstrup K, Andersen SB, Boelt B, Buus M, Gylling M, Holm PB, Kjellsson G, Pedersen S, Ostergard H, Mikkelsen SA (2003) Report from the Danish Working Group on the co-existence of genetically modifed crops with conventional and organic crops. DIAS Report Plant Production 94, p 275
Tynan, JL, Williams, MK, Conner, AJ (1990) Low frequency of pollen dispersal from a field trial of transgenic potatoes. J. Genet. Breed. 44: 303–306
Wilkinson, MJ, Davenport, IJ, Charters, YM (2000) A direct regional scale estimate of transgene movement from genetically modified oilseed rape to its wild progenitors. Mol. Ecol. 9: 983–991 CrossRef