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Diabrotica-resistant Bt-maize DKc5143 event MON88017 has no impact on the field densities of the leafhopper Zyginidia scutellaris

Published online by Cambridge University Press:  03 February 2011

Stefan Rauschen ,
Eva Schultheis ,
Heinz Hunfeld ,
Frank Schaarschmidt ,
Ingolf Schuphan  and
Sabine Eber
Stefan Rauschen*
Affiliation:
RWTH Aachen University, Department of Plant Physiology (Biology III), Worringerweg 1, 52074 Aachen, Germany
Eva Schultheis
Affiliation:
RWTH Aachen University, Department of Plant Physiology (Biology III), Worringerweg 1, 52074 Aachen, Germany
Heinz Hunfeld
Affiliation:
Institute for Applied Biotechnology in the Tropics at the University of Göttingen, 37077 Göttingen, Germany
Frank Schaarschmidt
Affiliation:
Leibniz Universität Hannover, Faculty of Natural Sciences, Institute of Biostatistics, Herrenhaeuser Straße 2, 30419 Hannover, Germany
Ingolf Schuphan
Affiliation:
RWTH Aachen University, Institute of Environmental Research (Biology V), Worringerweg 1, 52074 Aachen, Germany
Sabine Eber
Affiliation:
RWTH Aachen University, Department of Plant Physiology (Biology III), Worringerweg 1, 52074 Aachen, Germany
*
*Corresponding author: [email protected]

Abstract

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Auchenorrhyncha (planthoppers and leafhoppers) are herbivorous organisms that can ingest Cry proteins from genetically engineered Bt-crops depending on their feeding behaviour. Consequently, they might be directly affected by non-target Bt-protein action and more importantly serve as a source of Cry protein exposure to beneficial predatory arthropods. During a three year field study, we surveyed the community of Auchenorrhyncha in Diabrotica-resistant Bt-maize DKc5143-Bt (event MON88017), its near-isogenic line and two conventional hybrids using sweep netting and custom made sticky traps. Zyginidia scutellaris (Herrich-Schäffer) (Hemiptera: Cicadellidae) represented more than 60% of all captured individuals, indicating that it is the dominant leafhopper within the maize community. The statistical analysis of Z. scutellaris data using confidence intervals for the ratios of mean abundance showed no consistent differences between the Bt-maize and the near-isogenic cultivar, indicating no negative impact of event MON88017. The two conventional hybrids Benicia and DK315 exhibited differences in terms of Z. scutellaris densities, which were greater than those observed between MON88017 and the near-isogenic line, but also not consistent over the years. Six more species accounted for an additional 39% of all captured specimens, while ten more species were found only as single individuals and can be considered vagrants from neighbouring habitats. These results inform future field work on the non-target impact of Bt-maize on this group of arthropods and monitoring approaches to assess biological control function by surveying herbivore communities.

Keywords

Bt-maizeenvironmental risk assessmentMON88017non-target organismsZyginidia scutellaris
Type
Research Article
Information
Environmental Biosafety Research , Volume 9 , Issue 2 , April 2010 , pp. 87 - 99
Copyright
© ISBR, EDP Sciences, 2011

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