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A comparative investigation of the metabolism of the herbicide glufosinate in cell cultures of transgenic glufosinate-resistant and non-transgenic oilseed rape (Brassica napus) and corn (Zea mays)

Published online by Cambridge University Press:  15 October 2002

Monika Ruhland ,
Gabriele Engelhardt  and
Karlheinz Pawlizki
Monika Ruhland
Affiliation:
Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit, Außenstelle München, Menzinger Straße 54, 80638 München, Germany
Gabriele Engelhardt
Affiliation:
Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit, Außenstelle München, Menzinger Straße 54, 80638 München, Germany
Karlheinz Pawlizki
Affiliation:
Bayerische Landesanstalt für Bodenkultur und Pflanzenbau, Menzinger Straße 54, 80638 München, Germany

Abstract

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To obtain information on differences between the metabolic pathways of the herbicide glufosinate (trade names: BASTA®, LIBERTY®) in non-transgenic, glufosinate-sensitive plants and in transgenic, glufosinate-resistant plants, the metabolism of 14C-labeled glufosinate and its enantiomers L- and D-glufosinate was studied using cell cultures of oilseed rape and corn. Transformation of glufosinate in both sensitive and transgenic rape cells remained at a low rate of about 3-10% in contrast to corn cells, where 20% was transformed in sensitive and 43% in transgenic cells after 14 days of incubation, the rest remaining as unchanged glufosinate. In sensitive rape and corn cells the main metabolite was 4-methylphosphinico-2-oxo-butanoic acid (PPO) with 7.3 and 16.4%, respectively, together with low amounts of 3-methylphosphinicopropionic acid (MPP), 4-methylphosphinico-2-hydroxybutanoic acid (MHB), 4-methylphosphinicobutanoic acid (MPB) and 2-methylphosphinicoacetic acid (MPA). An additional metabolite formed in transgenic cell cultures was 2-acetamido-4-methylbutanoic acid (N-acetyl-L-glufosinate, NGA), which was formed at rates of 3.2% in rape and 16.1% in corn. A further minor metabolite, not yet identified, was detected in both cell types. The liberation of 0.2% 14CO2 indicates further metabolic steps prior to a limited mineralization in plant cell cultures. L-glufosinate was transformed into the same metabolites as the glufosinate racemate. D-glufosinate was not metabolized.

Keywords

glufosinateherbicide resistancemetabolismphosphinothricintransgenic plantscell cultures
Type
Research Article
Information
Environmental Biosafety Research , Volume 1 , Issue 1 , October 2002 , pp. 29 - 37
Copyright
© ISBR, EDP Sciences, 2002

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