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A Cytochemical Study of Glucose-6-Phosphatase (G-6-PASE) in Cells Participating in Atherogenesis of Rabbit Aorta

Published online by Cambridge University Press:  18 June 2020

Sidney D. Kobernick ,
Edna A. Elfont  and
Neddra L. Brooks
Sidney D. Kobernick
Affiliation:
Department of Laboratory Medicine, Electron Microscopy Laboratory, Sinai Hospital of Detroit, 6767 West Outer Drive, Detroit, Michigan48235
Edna A. Elfont
Affiliation:
Department of Laboratory Medicine, Electron Microscopy Laboratory, Sinai Hospital of Detroit, 6767 West Outer Drive, Detroit, Michigan48235
Neddra L. Brooks
Affiliation:
Department of Laboratory Medicine, Electron Microscopy Laboratory, Sinai Hospital of Detroit, 6767 West Outer Drive, Detroit, Michigan48235
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Extract

This cytochemical study was designed to investigate early metabolic changes in the aortic wall that might lead to or accompany development of atherosclerotic plaques in rabbits. The hypothesis that the primary cellular alteration leading to plaque formation might be due to changes in either carbohydrate or lipid metabolism led to histochemical studies that showed elevation of G-6-Pase in atherosclerotic plaques of rabbit aorta. This observation initiated the present investigation to determine how early in plaque formation and in which cells this change could be observed.

Male New Zealand white rabbits of approximately 2000 kg consumed normal diets or diets containing 0.25 or 1.0 gm of cholesterol per day for 10, 50 and 90 days. Aortas were injected jin situ with glutaraldehyde fixative and dissected out. The plaques were identified, isolated, minced and fixed for not more than 10 minutes. Incubation and postfixation proceeded as described by Leskes and co-workers.

Type
Cytochemistry and Electron Microscopy
Information
EMSA Proceedings , Volume 33: Proceedings Thirty-Third Annual Meeting Electron Microscopy Society of America, Las Vegas, Nevada August 11-15, 1975 , 15 1975 , pp. 460 - 461
Copyright
Copyright © Claitor’s Publishing Division 1975

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