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THE STOMATAL COMPLEX OF PODOCARPUS OBSERVED IN CROSS-SECTION USING CRYOFRACTURE: A PRELIMINARY STUDY

Published online by Cambridge University Press:  31 July 2017

M. Whiting ,
R. R. Mill  and
C. E. Jeffree
M. Whiting
Affiliation:
Royal Botanic Garden Edinburgh, 20A Inverleith Row, Edinburgh EH3 5LR, Scotland, UK. Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JH, Scotland, UK.
R. R. Mill*
Affiliation:
Royal Botanic Garden Edinburgh, 20A Inverleith Row, Edinburgh EH3 5LR, Scotland, UK.
C. E. Jeffree
Affiliation:
Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JH, Scotland, UK.
*
E-mail for correspondence: [email protected]
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Abstract

Cryofracture of living material and fracture at room temperature of herbarium material were used to obtain cross-sections of the stomatal complexes of four species of Podocarpus (Podocarpaceae) for scanning electron microscopy. Cross-sections of the stomata of one species in Podocarpus subgenus Foliolatus section Foliolatus (Podocarpus rubens), one in Podocarpus section Globulus (Podocarpus beecherae), one in Podocarpus subgenus Foliolatus section Longifoliolatus (Podocarpus insularis) and one in subgenus Podocarpus section Australis (Podocarpus nivalis) were studied. The architecture of the stomatal complex, including the wax plug, is described. It was found that the wax plug sits high in the stomatal antechamber in Podocarpus rubens, P. beecherae and Podocarpus decipiens and about halfway up the chamber in P. nivalis. A ridge, which appears to correspond to the crease where the guard cells meet, exists on the underside of the wax plug in Podocarpus beecherae, P. decipiens and P. rubens; its presence in P. nivalis requires confirmation. In addition, ridges within the stomatal antechamber were observed when viewing the cross-sections of Podocarpus decipiens and P. rubens, the internal surface of the cuticle of P. decipiens, Podocarpus teysmannii, P. insularis and Podocarpus milanjianus, and the external surface of the cuticle of Podocarpus chinensis, Podocarpus macrophyllus and Podocarpus pilgeri. These ridges may consist of wax and be a result of epitaxis.

Keywords

Cryofractureepistomatal chamberPodocarpaceaePodocarpusstomatastomatal antechamberwax plugs
Type
Articles
Information
Edinburgh Journal of Botany , Volume 74 , Issue 3 , November 2017 , pp. 345 - 364
Copyright
Copyright © Trustees of the Royal Botanic Garden Edinburgh (2017) 

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