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USE OF DIAGNOSTIC SSR MARKERS FOR IDENTIFICATION OF LARIX LYALLII AND L. OCCIDENTALIS (PINACEAE)

Published online by Cambridge University Press:  10 March 2003

S. NADEEM ,
B. JAQUISH ,
C. NEWTON  and
P. D. KHASA
S. NADEEM
Affiliation:
Department of Biology, South Building, Room 4026, University of Toronto at Mississauga, 3359 Mississauga Road N, Mississauga, Ontario L5L 1C6, Canada
B. JAQUISH
Affiliation:
Research Branch, B.C. Ministry of Forests, Kalamalka Research Station, 3401 Reservoir Road, Vernon, British Columbia V1B 2C7, Canada
C. NEWTON
Affiliation:
BC Research Inc., 3650 Wesbrook Mall, Vancouver, British Columbia V6S 2L2, Canada
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Abstract

Two species-specific microsatellite or simple sequence repeat (SSR) markers (UAKLly10a and UAKLla1) were used to distinguish Larix lyallii Parl. (subalpine larch) from L. occidentalis Nutt. (western larch) (Pinaceae). These markers can easily be used for rapid identification of the two species at any stage in the sporophyte phase of the life cycle. In the future, they should prove to be an invaluable tool for determining the possible occurrence and magnitude of introgressive hybridization in overlap zones between the two species. They are also expected to be useful in validating controlled crosses between L. lyallii and L. occidentalis and for certification purposes in reforestation and tree improvement programmes aimed at producing genetically improved hybrid stock.

Keywords

HybridizationLarixmicrosatellitesPCRsimple sequence repeats
Type
Research Article
Information
Edinburgh Journal of Botany , Volume 60 , Issue 1 , March 2003 , pp. 49 - 56
Copyright
2003, Trustees of the Royal Botanic Garden, Edinburgh

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