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Identification of toxic mineral dusts using mammalian cells

Published online by Cambridge University Press:  03 November 2011

R. Davies ,
M. Chamberlain ,
R. C. Brown  and
D. M. Griffiths
R. Davies
Affiliation:
R. Davies, M. Chamberlain, R. C. Brown and D. M. Griffiths, MRC Pneumoconiosis Unit, Llandough Hospital, Penarth, South Glamorgan, CF6 1XW, U.K.
M. Chamberlain
Affiliation:
R. Davies, M. Chamberlain, R. C. Brown and D. M. Griffiths, MRC Pneumoconiosis Unit, Llandough Hospital, Penarth, South Glamorgan, CF6 1XW, U.K.
R. C. Brown
Affiliation:
R. Davies, M. Chamberlain, R. C. Brown and D. M. Griffiths, MRC Pneumoconiosis Unit, Llandough Hospital, Penarth, South Glamorgan, CF6 1XW, U.K.
D. M. Griffiths
Affiliation:
R. Davies, M. Chamberlain, R. C. Brown and D. M. Griffiths, MRC Pneumoconiosis Unit, Llandough Hospital, Penarth, South Glamorgan, CF6 1XW, U.K.
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Abstract

Cell culture systems have been developed to assess the potential pathogenicity of mineral dusts. The in vitro cytotoxicities of a variety of dusts towards mouse peritoneal macrophages, Chinese hamster lung cells (V79 cell line) and human alveolar type II cells (A549 cell line) were investigated.

Non-pathogenic dusts were found to be inert in vitro. Fibrogenic non-fibrous dusts such as silica were only cytotoxic towards macrophages. Fibrous dusts which are both fibrogenic and carcinogenic in vivo are cytotoxic towards all three cell types, their cytotoxicity being dependent on fibre size. The size range important for the observed biological effect is longer than about 8 μm and thinner than about 1·5 μm.

Keywords

macrophagescell linesfibrosiscarcinogenesisasbestosdawsonitecytotoxicityfibres.
Type
Research Article
Information
Earth and Environmental Science Transactions of The Royal Society of Edinburgh , Volume 71 , Issue 3 , 1980 , pp. 181 - 184
Copyright
Copyright © Royal Society of Edinburgh 1980

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