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Efficacy of Two Texas Bentonites in Binding Aflatoxin B1 and in Reducing Aflatoxicosis in Broilers

Published online by Cambridge University Press:  01 January 2024

Ana Luisa Barrientos-Velazquez Open the ORCID record for Ana Luisa Barrientos-Velazquez [Opens in a new window] ,
Radhika Kakani ,
Justin Fowler ,
Akram-ul Haq ,
Christopher A. Bailey  and
Youjun Deng
Ana Luisa Barrientos-Velazquez
Affiliation:
Department of Soil & Crop Sciences, Texas A&M University, College Station, TX 77843-2474, USA
Radhika Kakani
Affiliation:
Department of Poultry Science, Texas A&M University, College Station, TX 77843-2472, USA
Justin Fowler
Affiliation:
Department of Poultry Science, Texas A&M University, College Station, TX 77843-2472, USA
Akram-ul Haq
Affiliation:
Department of Poultry Science, Texas A&M University, College Station, TX 77843-2472, USA
Christopher A. Bailey
Affiliation:
Department of Poultry Science, Texas A&M University, College Station, TX 77843-2472, USA
Youjun Deng*
Affiliation:
Department of Soil & Crop Sciences, Texas A&M University, College Station, TX 77843-2474, USA
*
e-mail: [email protected]
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Abstract

The incorporation of bentonites in aflatoxin-contaminated animal feeds to remedy aflatoxicosis has been tested widely in animal trials. Yet, a large variation in efficacy among samples has been observed which has been attributed to variations in the properties of the clay mineral adsorbents. The objectives of the current study were: (1) to evaluate the mineral and chemical composition of two selected bentonites to find minerals or elements which are potentially of concern; (2) to characterize the aflatoxin B1 (AfB1) adsorption (selectivity, capacity, reversibility, and interlayer accessibility) by the bentonites; and (3) to evaluate the safety and efficacy of selected clays as amendments of aflatoxin-contaminated feed for broiler chickens. The mineral, chemical, and exchange cation composition of the clays were analyzed, and they appeared to be safe for use in feed. The bentonites and their fractions showed that adsorption capacities range from 0.48 to 0.97 mol/kg. The interlayer spaces of both montmorillonites were accessible by AfB1, and the adsorption was irreversible. Three-day old broiler chickens were given clean and high-aflatoxin-concentration (1400 mg/kg) diets with and without the presence of the two bentonites. After three weeks the chickens were sacrificed and biomarkers were evaluated. The presence of aflatoxins reduced the body weight by 58% and resulted in a 25% mortality rate. Adding bentonites 1TX and 4TX increased the body weight of the chickens by 14 and 23%, respectively, but did not improve the mortality rates. The results suggested that selected bentonites could effectively sequester aflatoxins in vivo but did not eliminate the total toxicity present in highly contaminated poultry feed.

Keywords

AdsorptionAflatoxinBentonitePoultry feed
Type
Original Paper
Information
Clays and Clay Minerals , Volume 70 , Issue 3 , June 2022 , pp. 354 - 369
Copyright
Copyright © The Author(s), under exclusive licence to The Clay Minerals Society 2022

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