Hostname: page-component-cd9895bd7-fscjk Total loading time: 0 Render date: 2024-12-26T14:23:20.620Z Has data issue: false hasContentIssue false
  • English
  • Français

Adsorption of Protamine and Papain Proteins on Saponite

Published online by Cambridge University Press:  01 January 2024

Tamás Szabó ,
Raluca Mitea ,
Hugo Leeman ,
Gnanasiri S. Premachandra ,
Cliff T. Johnston ,
Márta Szekeres ,
Imre Dékány  and
Robert A. Schoonheydt
Tamás Szabó
Affiliation:
Department of Colloid Chemistry and Supramolecular and Nanostructured Materials Reseach Group of the Hungarian Academy of Sciences, University of Szeged, Aradi vértanúk tere 1, H-6720 Szeged, Hungary Center for Surface Chemistry and Catalysis, K.U. Leuven, Kasteelpark Arenberg 23, 3001 Leuven, Belgium
Raluca Mitea
Affiliation:
Center for Surface Chemistry and Catalysis, K.U. Leuven, Kasteelpark Arenberg 23, 3001 Leuven, Belgium
Hugo Leeman
Affiliation:
Center for Surface Chemistry and Catalysis, K.U. Leuven, Kasteelpark Arenberg 23, 3001 Leuven, Belgium
Gnanasiri S. Premachandra
Affiliation:
Department of Crop, Soil and Environmental Sciences and Birck Nanotechnology Center, Purdue University, 915 West State Street, West-Lafayette, Indiana 47907-2054, USA
Cliff T. Johnston
Affiliation:
Department of Crop, Soil and Environmental Sciences and Birck Nanotechnology Center, Purdue University, 915 West State Street, West-Lafayette, Indiana 47907-2054, USA
Márta Szekeres
Affiliation:
Department of Colloid Chemistry and Supramolecular and Nanostructured Materials Reseach Group of the Hungarian Academy of Sciences, University of Szeged, Aradi vértanúk tere 1, H-6720 Szeged, Hungary
Imre Dékány
Affiliation:
Department of Colloid Chemistry and Supramolecular and Nanostructured Materials Reseach Group of the Hungarian Academy of Sciences, University of Szeged, Aradi vértanúk tere 1, H-6720 Szeged, Hungary
Robert A. Schoonheydt*
Affiliation:
Center for Surface Chemistry and Catalysis, K.U. Leuven, Kasteelpark Arenberg 23, 3001 Leuven, Belgium
*
* E-mail address of corresponding author: [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Due to the increased importance of bionanocomposites, protamine and papain proteins were adsorbed on Na+- and on Cs+-exchanged saponite from aqueous solution. Protein analysis of equilibrium solutions and thermogravimetric analyses of biocomposites were used to prepare adsorption isotherms. Based on the isotherm shape, and on the amounts of protein adsorbed and the amounts of Na+ and Cs+ released, the initial protein sorption apparently was due to ion exchange. Additional sorbed protein was weakly retained and could be removed by washing with water. From ion exchange, the average charge of the protamine adsorbed was estimated to be +13.1 to +13.5. Similar papain measurements could not be made due to partial decomposition. Quantitatively, protamine was adsorbed at levels up to 400 mg/g on Na+-saponite and 200 mg/g on Cs+-saponite. The maximum protamine adsorption was 650 to 700 mg/g for Na+-saponite and 350–400 mg/g for Cs+-saponite. Protamine was sorbed to edge surfaces and the basal spacing of the interlamellar region of saponite was 1.75 nm. Protamine displaced only 36% of the Cs+ in Cs+-saponite and expanded the interlamellar region by 36% for a basal spacing of 1.6 nm. Papain sorption to Na+-saponite occurred by a two-step process: (1) adsorption to saponite particle external surfaces followed, (2) by partial intercalation. Quantitatively, Papain was adsorbed up to 100 mg/g for Na+-and Cs+-saponite. Greater initial papain concentrations resulted in a 450 mg/g maximum for Na+-saponite, but no increase above 100 mg/g for Cs+-saponite. Papain apparently only sorbed to external Cs+-saponite surfaces that were estimated to be 33–40 m2/g. Step-wise thermal decomposition of the saponite-protein composites occurred between 300 and 800°C.

Keywords

IntercalationIon ExchangePapainProtamineSaponite
Type
Article
Information
Clays and Clay Minerals , Volume 56 , Issue 5 , October 2008 , pp. 494 - 504
Copyright
Copyright © The Clay Minerals Society 2009

References

Amemiya, S. Yang, X. and Wazenegger, T.L., 2003 Voltammetry of phase transfer of polypeptide protamines across polarized liquid/liquid interfaces Journal of the American Chemical Society 125 832–833 10.1021/ja036572b.CrossRefGoogle ScholarPubMed
Blomberg, E. Claesson, P.M. Froberg, J.C. and Tilton, R.D., 1994 Interaction between adsorbed layers of lysozyme with the surface force technique Langmuir 10 2325–2334 10.1021/la00019a050.CrossRefGoogle Scholar
Brack, A., Bergaya, F. Lagaly, G. and Theng, B.K.G., 2006 Clay minerals and the origin of life Handbook of Clay Sciences Amsterdam Elsevier 379–391 10.1016/S1572-4352(05)01011-1.CrossRefGoogle Scholar
Gray, J.J., 2004 The interaction of proteins with solid surfaces Current Opinion in Structural Biology 14 110–115 10.1016/j.sbi.2003.12.001.CrossRefGoogle ScholarPubMed
Harpaz, Y. Gerstein, M. and Chothia, C., 1994 Volume changes on protein-folding Structure 2 641–649 10.1016/S0969-2126(00)00065-4.CrossRefGoogle ScholarPubMed
Hudson, S. Magnier, E. Cooney, J. and Hodnett, B.K., 2005 Methodology for the Immobilization of Enzymes on Porous Materials Journal of Physical Chemistry B 109 19496–19506 10.1021/jp052102n.CrossRefGoogle Scholar
Jaynes, W.F. and Boyd, S.A., 1991 Hydrophobicity of siloxane surfaces in smectites as revealed by aromatic hydrocarbon adsorption from water Clays and Clay Minerals 39 428–436 10.1346/CCMN.1991.0390412.CrossRefGoogle Scholar
Kim, D.T. Blanch, H.W. and Radke, C.J., 2002 Direct imaging of lysozyme onto mica by AFM Langmuir 18 5841–5850 10.1021/la0256331.CrossRefGoogle Scholar
Laird, D.A. Barriuso, E. Dowdy, R.H. and Koskinen, W.C., 1992 Adsorption of atrazine on smectites Soil Science Society of America Journal 56 62–67 10.2136/sssaj1992.03615995005600010010x.CrossRefGoogle Scholar
Maes, A. and Cremers, A., 1978 Charge density effects in ion exchange. Part 2. Homovalent exchange equilibria Journal of the Chemical Society. Faraday Transactions 74 1234–1242 10.1039/f19787401234.CrossRefGoogle Scholar
Mortland, M.M. Fripiat, J.J. Chaussidon, J. and Uytterhoeven, J.B., 1963 Interaction between ammonia and the expanding lattices of montmorillonite and vermiculite Journal of Physical Chemistry 67 248–258 10.1021/j100796a009.CrossRefGoogle Scholar
Nulens, K.H.L. Toufar, H. Janssens, G.O.A. Schoonheydt, R.A. Johnston, C.T., Yamagishi, A. Aramata, A. and Taniguchi, M., 1996 Clay minerals and clay mineral-water interaction: a combined EEM-Monte Carlo study The latest Frontiers of Clay Chemistry Sendai The Smectite Forum of Japan 116–133.Google Scholar
Ottensmeyer, F.P. Whiting, R.F. and Kron, A.P., 1975 Three-dimensional structure of herring sperm protamine Y-I with the aid of dark field electron microscopy Proceedings of the National Academy of Sciences of the United States of America 72 4953–4955 10.1073/pnas.72.12.4953.CrossRefGoogle ScholarPubMed
Quiquampoix, H. Staunton, S. Baron, M.-H. and Ratcliffe, R.G., 1993 Interpretation of the pH dependence of protein adsorption on clay surfaces Colloids and Surfaces A 75 85–93 10.1016/0927-7757(93)80419-F.CrossRefGoogle Scholar
Rasor, P., De Vos, D. Vankelecom, I.F.J. and Jacobs, P.A., 2000 Immobilized enzymes in enantioselective organic synthesis Chiral Catalyst Immobilization and Recycling Weinheim, Germany Wiley-VCH 97–122.CrossRefGoogle Scholar
Ruiz-Hitzky, E. Ariya, K. and Lvov, J.M., 2008 Bio-inorganic Hybrid Materials. Strategies, Synthesis, Characterization and Applications Weinheim, Germany Wiley 500 pp.Google Scholar
Szabó, T. Szekeres, M. Dékány, I. Jackers, C. De Feyter, S. Johnston, C.T. and Schoonheydt, R.A., 2007 Layer-by-layer construction of ultrathin hybrid films with proteins and clay minerals Journal of Physical Chemistry C 111 12730–12740 10.1021/jp0722861.CrossRefGoogle Scholar
Theng, B.K.G., 1974 Clay-Organic Interactions London A. Hilger 343 pp.Google Scholar
Trojanek, A. Langmaier, J. Samcova, E. and Samec, Z., 2007 Counterion binding to protamine polyion at a polarized liquid-liquid interface Journal of Electroanalytical Chemistry 603 235–242 10.1016/j.jelechem.2007.02.006.CrossRefGoogle Scholar
Van Olphen, H. and Fripiat, J.J., 1979 Data Handbook for Clay Materials and other Non-Metallic Materials Oxford, UK Pergamon 346 pp.Google Scholar
Wertz, C.F. and Santore, M.M., 2001 Effect of surface hydrophobicity on adsorption and relaxation kinetics of albumin and fibrinogen: single species and competitive adsorption Langmuir 17 3006–3016 10.1021/la0017781.CrossRefGoogle Scholar
Wertz, C.F. and Santore, M.M., 2002 Fibrinogen adsorption on hydrophilic and hydrophobic surfaces: geometrical and energetical aspects of interfacial relaxations Langmuir 18 706–715 10.1021/la011075z.CrossRefGoogle Scholar
Wertz, C.F. and Santore, M.M., 2002 Adsorption and reorientation kinetics of lysozyme on hydrophobic surfaces Langmuir 18 1190–1199 10.1021/la0108813.CrossRefGoogle Scholar
Zhao, X.S. Bao, X.Y. Guo, W. and Lei, F.Y., 2006 Immobilizing catalysts on porous materials Materials Today 9 32–39 10.1016/S1369-7021(06)71388-8.CrossRefGoogle Scholar