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Transformation of potato using an antisense class I patatin gene and its effect on microtuber formation

Published online by Cambridge University Press:  12 February 2007

Si Huai-Jun ,
Liu Jun  and
Xie Cong-Hua
Si Huai-Jun
Affiliation:
College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, China College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
Liu Jun
Affiliation:
College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Xie Cong-Hua*
Affiliation:
College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, China
*
*Corresponding author: Email: [email protected]
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Abstract

An antisense class I patatin gene under control of the CaMV 35S promoter was introduced into potato (Solanum tuberosum) cultivar E-potato 3 using the Agrobacterium tumefaciens system. PCR amplification and PCR–Southern blot analysis indicated that the antisense class I patatin gene had been integrated into the potato genome. Northern hybridization analysis showed that the antisense gene transcribed normally in the transgenic potato plants and resulted in a reduction of endogenous class I patatin mRNA. Total soluble protein content and lipid acyl hydrolase activity of microtubers, derived from transformed plants, decreased by a maximum of 36.4% and 31.4%, respectively, compared with control plants. The expression of this antisense gene also resulted in reductions of the plantlets forming tubers, tubers per plantlet and the effective tubers (≥50 mg) of the transformed plants.

Keywords

potatoclass I patatin geneantisense RNAmicrotuber
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 2 , Issue 1 , April 2005 , pp. 7 - 11
Copyright
Copyright © China Agricultural University and Cambridge University Press 2005

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