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Simultaneous detection of bovine- and sheep-derived materials in feedstuffs by PCR

Published online by Cambridge University Press:  12 February 2007

Wen Wei-Gang ,
Zhao Xiu-Ling ,
He Shui-Shan ,
Du Hua-Hong  and
Huang Shao-Tang
Wen Wei-Gang*
Affiliation:
Ningbo Entry–exit Inspection and Quarantine Bureau, Ningbo 315012, China
Zhao Xiu-Ling
Affiliation:
Ningbo Entry–exit Inspection and Quarantine Bureau, Ningbo 315012, China
He Shui-Shan
Affiliation:
Ningbo Entry–exit Inspection and Quarantine Bureau, Ningbo 315012, China
Du Hua-Hong
Affiliation:
Ningbo Entry–exit Inspection and Quarantine Bureau, Ningbo 315012, China
Huang Shao-Tang
Affiliation:
Ningbo Entry–exit Inspection and Quarantine Bureau, Ningbo 315012, China
*
*Corresponding author: Email: [email protected]
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Abstract

To control the spread of bovine spongiform encephalopathy and scrapie, a method was developed for the simultaneous detection of bovine- and sheep-derived materials in feedstuffs. Chelex-100 was adopted for the fast extraction of animal genomic DNA. A pair of universal primers, designed on the basis of homology in sequences of bovine and sheep mtDNA, was used for PCR. This method is of great value to quarantine departments for its simplicity and rapid detection.

Keywords

Chelex-100PCRuniversal primersbovine- and sheep-derived materialssimultaneous detection
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 2 , Issue 1 , April 2005 , pp. 13 - 16
Copyright
Copyright © China Agricultural University and Cambridge University Press 2005

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