Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-09T01:36:41.705Z Has data issue: false hasContentIssue false
  • English
  • Français

RNAi effects on regulation of endogenous acid invertase activity in potato (Solanum tuberosum L.) tubers

Published online by Cambridge University Press:  01 October 2008

Zhang Chi ,
Xie Cong-Hua ,
Song Bo-Tao ,
Liu Xun  and
Liu Jun
Zhang Chi
Affiliation:
National Vegetable Improvement Center (Central China), Key Laboratory of Horticultural Plant Biology, Ministry of Education, Potato Engineering and Technology Research Center of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China
Xie Cong-Hua
Affiliation:
National Vegetable Improvement Center (Central China), Key Laboratory of Horticultural Plant Biology, Ministry of Education, Potato Engineering and Technology Research Center of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China
Song Bo-Tao
Affiliation:
National Vegetable Improvement Center (Central China), Key Laboratory of Horticultural Plant Biology, Ministry of Education, Potato Engineering and Technology Research Center of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China
Liu Xun
Affiliation:
National Vegetable Improvement Center (Central China), Key Laboratory of Horticultural Plant Biology, Ministry of Education, Potato Engineering and Technology Research Center of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China
Liu Jun*
Affiliation:
National Vegetable Improvement Center (Central China), Key Laboratory of Horticultural Plant Biology, Ministry of Education, Potato Engineering and Technology Research Center of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China
*
*Corresponding author. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

In plants, acid invertases are known to be the key enzymes cleaving sucrose into reducing sugars (RS) (glucose and fructose). To improve the quality of potato (Solanum tuberosum L.) chips, which is largely influenced by RS accumulation in tubers stored at low temperature, a part of acid invertase cDNA with hairpin RNA (hpRNA) structure was transformed into potato cv. N2. Detection of polymerase chain reaction (PCR) amplification and Northern blotting suggested that the RNA interference (RNAi) vector was successfully transformed into cv. N2. The analysis of acid invertase activity in the plantlets and microtubers of RNAi transgenic lines indicated that the expression of the acid invertase was significantly repressed by the activity of RNAi of plantlets by an average 69.8% (with the exception of line Ni-1) with a maximal decrease of 78% (line Ni-4), and the highest decrease of activity in microtubers of 68%. Compared with that of well-inhibited antisense inv transgenic plants, the comparative downregulation of RNAi suggests a distinct alteration of endogenous acid invertase activity and a potential strategy for post-transcriptional gene silencing (PTGS) in modulation of cold-sweetening in potato.

Keywords

potato (Solanum tuberosum L.)acid invertasecold-sweeteningRNA interference
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 2 , August 2008 , pp. 107 - 112
Copyright
Copyright © China Agricultural University 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

First published in Journal of Agricultural Biotechnology 2008, 16(1): 108–113

References

Arthur, K, Napoli, C, Jorgensen, R and Müller, A (2004) Effectiveness of RNA interference in transgenic plants. FEBS Letters 566: 223–228.Google Scholar
Chen, F and Hu, XS (2000) The effect of storage temperature on carbohydrate content and chip color of the potato tuber. Acta Horticulturae Sinica 27(3): 218–219.Google Scholar
Cheng, SH, Su, ZH, Liu, J and Xie, CH (2004) Effects of variation in activities of starch-sugar metabolic enzymes on reducing sugar accumulation and processing quality of potato tubers. Agricultural Sciences in China 37(12): 1904–1910.Google Scholar
Cheng, SH, Liu, J, Xie, CH, Song, BT, Liu, X and Li, JC (2006) Role of tobacco vacuolar invertase regulated by patatin promoter in resistance of potato tubers to cold-sweetening. Journal of Agricultural Biotechnology 14(5): 716–720.Google Scholar
Fire, A, Xu, S, Montgomery, MK, Kostas, SA, Driver, SE and Meilo, CC (1998) Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391(6669): 806–811.CrossRefGoogle ScholarPubMed
Greiner, S, Rausch, T, Sonnewald, U and Herbers, K (1999) Ectopic expression of a tobacco invertase inhibitor homolog prevents cold-induced sweetening of potato tubers. Nature Biotechnology 17: 708–711.CrossRefGoogle ScholarPubMed
Hofgen, R and Willmitzer, L (1988) Storage of competent cells for Agrobacterium transformation. Nucleic Acids Research 16: 9877.CrossRefGoogle ScholarPubMed
Kawata, M, Matsumura, Y, Oikawa, T, Kimizu, M, Fukumoto, F and Kuroda, S (2003) Analysis of DNA extraction buffer components from plant tissue by polymerase chain reaction. Analytical Biochemistry 318: 314–317.CrossRefGoogle ScholarPubMed
Li, L, Strahwald, J, Hofferbert, HR, et al. (2005) DNA variation at the invertase locus in invGE/GF is associated with tuber quality traits in population of potato breeding clones. Genetics 170: 813–821.CrossRefGoogle ScholarPubMed
Liu, J and Xie, CH (2001) The mechanism of potato (Solanum tuberosum L.) tuber development and related gene expression. Chinese Bulletin of Botany 18(5): 531–539.Google Scholar
Lorberth, R, Ritte, G, Willmitzer, L and Kossmann, J (1998) Inhibition of a starch-granule-bound protein leads to modified starch and repression of cold sweetening. Nature Biotechnology 16: 473–477.CrossRefGoogle ScholarPubMed
Menendez, CM, Ritter, E, Schafer-Pregl, R, et al. (2002) Cold sweetening in diploid potato: mapping quantitative trait loci and candidate genes. Genetics 162: 1423–1434.CrossRefGoogle ScholarPubMed
Miki, D and Shimamoto, K (2004) Simple RNAi vectors for stable and transient suppression of gene function in rice. Plant and Cell Physiology 45: 490–495.CrossRefGoogle ScholarPubMed
Si, HJ, Xie, CH and Liu, J (2003) An efficient protocol for Agrobacterium-mediated transformation with microtuber and the induction of an antisense class I patatin gene into potato. Acta Agronomica Sinica 29: 801–805.Google Scholar
Stark, DM, Timmermann, KP, Barry, GF, Preiss, J, Kishore, GM (1992) Regulation of the amount of starch in plant tissues by ADP glucose pyrophosphorylase. Science 258: 287–292.CrossRefGoogle ScholarPubMed
Sturm, A (1999) Invertases, primary structures, functions and roles in plant development and sucrose partitioning. Plant Physiology 121: 1–8.CrossRefGoogle ScholarPubMed
Szittya, G, Silhavy, D, Molnár, A, et al. (2003) Low temperature inhibits RNA silencing-mediated defense by the control of siRNA generation. EMBO Journal 22(3): 633–640.CrossRefGoogle ScholarPubMed
Thomas, CL, Jones, L, Baulcombe, DC and Maule, AJ (2001) Size constrains for targeting post-transcriptional gene silencing and for RNA-directed methylation in Nicotiana benthamiana using a potato virus X vector. Plant Journal 25(4): 417–425.CrossRefGoogle Scholar
Wassenegger, M and Pelissier, T (1998) A model for RNA-mediated gene silencing in higher plants. Plant Molecular Biology 37: 349–362.CrossRefGoogle Scholar
Zrenner, R, Schuler, K and Sonnewald, U (1996) Soluble acid invertase determines the hexose-to-sucrose ratio in cold-stored potato tubers. Planta 198(2): 246–252.CrossRefGoogle ScholarPubMed