Hostname: page-component-cd9895bd7-dzt6s Total loading time: 0 Render date: 2024-12-28T12:28:36.116Z Has data issue: false hasContentIssue false
  • English
  • Français

Polymorphism of porcine uncoupling protein 3 (UCP3) and association with fat deposition and meat quality

Published online by Cambridge University Press:  15 June 2007

Ren Liang ,
Zhu Bao-Qin ,
Han Dan ,
Zhang Yi-Bo ,
Song Hui-Juan ,
Zeng Rui-Xia  and
Su Yu-Hong
Ren Liang
Affiliation:
Key Laboratory of Molecular Cell Biology and New Drug of Liaoning High Education, JinZhou Medical College, Jinzhou 121000, China
Zhu Bao-Qin
Affiliation:
Key Laboratory of Molecular Cell Biology and New Drug of Liaoning High Education, JinZhou Medical College, Jinzhou 121000, China
Han Dan
Affiliation:
Key Laboratory of Molecular Cell Biology and New Drug of Liaoning High Education, JinZhou Medical College, Jinzhou 121000, China
Zhang Yi-Bo
Affiliation:
Key Laboratory of Molecular Cell Biology and New Drug of Liaoning High Education, JinZhou Medical College, Jinzhou 121000, China
Song Hui-Juan
Affiliation:
Key Laboratory of Molecular Cell Biology and New Drug of Liaoning High Education, JinZhou Medical College, Jinzhou 121000, China
Zeng Rui-Xia
Affiliation:
Key Laboratory of Molecular Cell Biology and New Drug of Liaoning High Education, JinZhou Medical College, Jinzhou 121000, China
Su Yu-Hong*
Affiliation:
Key Laboratory of Molecular Cell Biology and New Drug of Liaoning High Education, JinZhou Medical College, Jinzhou 121000, China
*
*Corresponding author. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Based on quantitative trait locus (QTL) mapping, the gene for porcine uncoupling protein 3 (UCP3) was chosen as a candidate gene for pig fat deposition and meat quality traits. In this study, a partial coding region of the UCP3 gene was sequenced and one single nucleotide polymorphism (cSNP) was found at 395 bp. The mutation was G→A and resulted in the amino acid change from glycine to arginine. This site was also recognized by restriction endonuclease SmaI. The UCP3 SmaI polymorphism was analysed among 186 individuals of Large White×Meishan F2 progeny using polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP). The genotypes of the UCP3 SmaI polymorphism were AA, AB and BB. The frequency of A and B alleles was respectively 0.56 and 0.44. Statistical analyses showed that the SmaI polymorphism in the F2 population was significantly associated with back-fat thickness at thorax–waist and buttock, as well as with intramuscular fat, drip-loss rate and water-holding capacity. The additive effect of UCP3 SmaI was clearly shown. The genotype AA reduced back-fat thickness and drip-loss rate, increased water-holding capacity, and decreased the intramuscular fat. The effect of the pig UCP3 SmaI polymorphism needs to be analysed in other populations using larger samples.

Keywords

Sus scrofauncoupling protein 3PCR-RFLPgenetic effect analysis
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 4 , Issue 1 , April 2007 , pp. 47 - 51
Copyright
Copyright © China Agricultural University and Cambridge University Press 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

First published in Journal of Agricultural Biotechnology 2006, 14(5): 652–656

References

Boss, O and Hagen, T (2000) Uncoupling proteins 2 and 3, potential regulators of mitochondrial energy metabolism. Diabetes 49: 143–156.CrossRefGoogle ScholarPubMed
Damon, M, Vincent, A, Lombardi, A and Herpin, P (2000) First evidence of uncoupling protein-2 (UCP-2) and -3 (UCP-3) gene exoression in piglet skeletal muscle adipose tissue. Gene 246: 133–141.CrossRefGoogle Scholar
Fang, MY, Zhao, XB, Li, N and Wu, CX (2002) Genetic variations of 3′ regulatory region of uncoupling protein 3 from different pig breeds. Chinese Science Bulletin 47(13): 1010–1012 (in Chinese).CrossRefGoogle Scholar
Knoll, A, Putnova, J, Dvorak, J, Rohrer, GA and Cepica, S (2000) Linkage mapping of an AvaI PCR-RFLP within the porcine uncoupling protein 3 gene. Animal Genetics 31(2): 156–157.CrossRefGoogle Scholar
Liu, BH (1998) Statistical Genomics: Linkage, Mapping and QTL Analysis. Boca Raton, CRC Press, pp. 404–409.Google Scholar
Shen, HL, Xiang, KS and Jia, WP (2002) Effects of uncoupling protein3 gene −55C→T variant on lipid metabolism, body fat, its distribution and non-insulin-dependent diabetes mellitus in Chinese. Chinese Journal of Medical Genetics 19(4): 317–321 (in Chinese with English abstract).Google Scholar
Su, YH, Xiong, YZ, Zhang, Q, et al. (2002) Mapping quantitative trait loci for fat deposition in carcass in pigs. Acta Genetica Sinica 29(8): 681–684 (in Chinese with English abstract).Google ScholarPubMed
Tu, RJ, Deng, CY and Xiong, YZ (2004) Sequencing analysis on partial translated region of uncoupling protein 3 gene and single nucleotide polymorphism associations with the porcine carcass and meat quality traits. Acta Genetica Sinica 31(8): 807–812 (in Chinese with English abstract).Google ScholarPubMed
Webb, AJ (1998) Objectives and strategies in pig improvement and applied perspective. Journal of Dairy Science 81(Suppl. 2): 36–46.CrossRefGoogle ScholarPubMed
Werner, P, Neuenschwander, S and Stranzinger, G (1999) Characterization of the porcine uncoupling proteins 2 and 3 and their localization to chromosome 9 by somatic cell hybrids. Animal Genetics 30(3): 221–224.CrossRefGoogle ScholarPubMed
Zuo, B, Xiong, YZ, Su, YH, Deng, CY, Yu, L and Zheng, R (2003) Genetic linkage map construction of some microsatellites on pig chromosome 4 and 7. Journal of Agricultural Biotechnology 11(1): 70–74 (in Chinese with English abstract).Google Scholar