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Induction of lamb follicular development and embryo production in vitro

Published online by Cambridge University Press:  03 March 2009

Chen Xiao-Yong ,
Tian Shu-Jun ,
Sang Run-Zi ,
Sun Shu-Chun ,
Zhao Zhu-Jun ,
Li Jun-Jie  and
Dun Wei-Tao
Chen Xiao-Yong
Affiliation:
College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071001, China
Tian Shu-Jun*
Affiliation:
College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071001, China Embryo Engineering and Technological Center of Cattle and Sheep of Hebei, Baoding 071001, China
Sang Run-Zi
Affiliation:
College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071001, China Embryo Engineering and Technological Center of Cattle and Sheep of Hebei, Baoding 071001, China
Sun Shu-Chun
Affiliation:
College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071001, China Embryo Engineering and Technological Center of Cattle and Sheep of Hebei, Baoding 071001, China
Zhao Zhu-Jun
Affiliation:
College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071001, China Embryo Engineering and Technological Center of Cattle and Sheep of Hebei, Baoding 071001, China
Li Jun-Jie
Affiliation:
College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071001, China Embryo Engineering and Technological Center of Cattle and Sheep of Hebei, Baoding 071001, China
Dun Wei-Tao
Affiliation:
Academy of Hebei Animal Husbandry and Veterinary Medicine, Baoding 071000, China
*
*Corresponding author. E-mail: [email protected]
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Abstract

Effect of lamb age, transport stimulation and repeated hormone superovulation on the number of collected oocytes were determined. Effect of the culture medium containing ethylenediaminetetraacetic acid (EDTA) on the development of embryos produced in vitro from lamb oocytes was also investigated. Results indicated that the mean numbers of collected and available oocytes (oocytes with compacted cumulus cells, evenly granulated ooplasm and morphologically normal) from 6- to 8-week-old lambs were 60.8±13.9 and 58.2±12.3, respectively. These figures were higher than those obtained from 12- to 14-week-old lambs (27.3±5.1 and 26.0±4.9) (P<0.05). Stimulation by transportation didn't decrease the number of collected oocytes from the superovulated lambs (P>0.05). However, the number of collected oocytes in the repeated superovulation group was significantly reduced, compared with the control group (P<0.05). The embryonic culture medium supplemented with 10 μmol/l EDTA highly improved the development capability of the embryos from lamb oocytes (P<0.05). Healthy lambs were born following embryo transfer.

Keywords

juvenile lamboocyteembryo
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 3 , December 2008 , pp. 257 - 262
Copyright
Copyright © China Agricultural University 2008

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Footnotes

First published in Journal of Agricultural Biotechnology 2008, 16(3): 456–460

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