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In vitro selection of herbicide resistance in microspore-derived embryos of oilseed rape (Brassica napus L.)

Published online by Cambridge University Press:  12 February 2007

Xu Ling ,
Zhang Guo-Qing ,
Gu Hong-Hui ,
He Yun  and
Zhou Wei-Jun
Xu Ling
Affiliation:
Agronomy Department, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China
Zhang Guo-Qing
Affiliation:
Agronomy Department, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China National Agro-Tech Extension and Service Center, Ministry of Agriculture, Beijing 100026, China
Gu Hong-Hui
Affiliation:
Agronomy Department, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China Institute of Crop Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
He Yun
Affiliation:
Agronomy Department, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China
Zhou Wei-Jun*
Affiliation:
Agronomy Department, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China
*
*Corresponding author: E-mail: [email protected]
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Abstract

Using four oilseed rape (Brassica napus L.) F1 hybrids (7039, 7040, 282 and 5102) as donor plants for microspore culture, the experiment was conducted to select glyphosate- and haloxyfop-resistant embryos through application of these substances to the cultural media with microspore-derived embryos in vitro. Genotypes 7039 and 7040 were used to select glyphosate-resistant regenerated plants, and genotypes 282 and 5102 to select haloxyfop-resistant plants. The embryos at cotyledonary stage were grown on glyphosate- and haloxyfop-containing MS-2 medium for 2 weeks. The non-resistant embryos collapsed after a short time, while the resistant ones turned green and survived for 2 weeks. Transferred into the normal MS-2 medium for further plant regeneration, the regenerated plants from green embryos showed tolerance to 0.25% sprayed glyphosate, indicating the effectiveness and reliability of this in vitro selection method. When the regenerated plants selected from 0.02% haloxyfop were sprayed with 0.05% haloxyfop, most of them grew well; however, the survival rate of the regenerated plants from 0.01% haloxyfop-containing medium was lower. The present experiment indicates that the use of 0.02% haloxyfop in the selection of haloxyfop-resistant plants was more promising than that of 0.01%. The chromosome doubling efficiency of regenerated plants reached 34% and 52% after being treated with 170 mg/l colchicine for 20 and 30 h, respectively.

Keywords

Brassica napusembryoglyphosate resistancehaloxyfop resistancein vitro selectionmicrospore cultureplant regeneration
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 3 , Issue 1 , April 2006 , pp. 33 - 38
Copyright
Copyright © China Agricultural University and Cambridge University Press 2006

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