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In vitro effect of lentinan on the activation of immunological cells in Cyprinus carpio

Published online by Cambridge University Press:  24 April 2009

Zhang Liu ,
Cao Li-Ping ,
Ding Wei-Dong ,
Galina Jeney ,
Xu Pao ,
Xiong Chuan-Xi  and
Yin Guo-Jun
Zhang Liu
Affiliation:
Hua zhong Agricultural University, Wuhan 430070, China Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Science, Wuxi 214081, China
Cao Li-Ping
Affiliation:
Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Science, Wuxi 214081, China
Ding Wei-Dong
Affiliation:
Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Science, Wuxi 214081, China
Galina Jeney
Affiliation:
Research institute for Fisheries, Aquaculture and Irrigation, Anna light 8, Szarvas H-4440, Hungary
Xu Pao
Affiliation:
Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Science, Wuxi 214081, China
Xiong Chuan-Xi*
Affiliation:
Hua zhong Agricultural University, Wuhan 430070, China
Yin Guo-Jun*
Affiliation:
Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Science, Wuxi 214081, China
*
*Corresponding authors. E-mail: [email protected] and [email protected]
*Corresponding authors. E-mail: [email protected] and [email protected]
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Abstract

Macrophages from the head kidney (HK) and peripheral blood leucocytes were isolated fromCyprinus carpio by Percoll gradient density centrifugation, cultured in vitro and exposed to different concentrations of the immunomodulator lentinan. To evaluate the immunostimulating effects of lentinan, proliferation of the peripheral blood leucocytes, respiratory burst of macrophages and interleukin-1β (IL-1β) gene mRNA expression of macrophages were investigated by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide], NBT (nitroblue tetrazolium) reduction, Griess reaction and real-time polymerase chain reaction (PCR). Results showed that proliferation of peripheral blood leucocytes after 24 h incubation by the induction of lentinan at 100 and 1000 μg/ml was markedly stimulated. Lentinan at 1, 100 and 1000 μg/ml could significantly induce superoxide anions in macrophages. Production of nitric oxide by HK macrophages after 96 h incubation by lentinan showed that it had no conspicuous effect on nitrogen burst activity of macrophages. Moreover, it inhibited the nitrogen burst activity at higher doses. The expression of IL-1β in the HK macrophages after 24 h of polysaccharide stimulation showed that lentinan stimulated IL-1β expression in the head kidney in carp. Lentinan can modulate the immune response of C. carpio.

Keywords

lentinanCyprinus carpiomacrophageIL-1β
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 6 , Issue 1 , April 2009 , pp. 69 - 74
Copyright
Copyright © China Agricultural University 2009

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References

Bayne, CJ (1986) Pronephric leucocytes of Cyprinus carpio: isolation, separation and characterization. Veterinary Immunology and Immunopathology 12: 141–151.CrossRefGoogle ScholarPubMed
Cohen-Kupiec, R, Broglie, KE, Friesem, D, Broglie, RM and Chet, I (1999) Molecular characterization of a novel β-1,3-exoglucanase related to mycoparasitism of Trichoderma harzianum. Gene 226: 147–154.CrossRefGoogle ScholarPubMed
Ding, H, Hou, G and Zhou, KY (2003) Effects of lentinan on the nitric oxide production and the inducible nitric oxide synthase in mouse peritoneal macrophages. Guangdong Pharmaceutical Journal 13(4): 32–33 (in Chinese with English abstract).Google Scholar
Dubois, M, Gilles, KA, Hamilton, JK, et al. (1956) Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28: 350–356.CrossRefGoogle Scholar
Jin, BQ (2001) Cellular and Molecular Immunology. Beijing: Science Press, 131–239 (in Chinese).Google Scholar
Kun, YL and Young, JJ (2005) Macrophage activation by polysaccharide isolated from Astragalus membranaceus. International Immunopharmacology 5(7): 1225–1233.Google Scholar
Liu, HB (2002) Present status of Chinese herbal medicine immunopotentiator and its application in aquaculture. Journal of Aquaculture 12(210): 121–127.Google Scholar
Liu, MQ, Li, JZ, Fanzuo, K, Lin, JY and Gao, Y (1998) Induction of immunomodulating cytokines by a new polysaccharide–peptide complex from culture mycelia of Lentinus edodes. Immunopharmacology 40(3): 187–198.CrossRefGoogle ScholarPubMed
Livak, KJ and Schmittgen, TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods 25: 402–408.CrossRefGoogle Scholar
Maruyama, S, Sukekawa, Y, Kaneko, Y, et al. (2006) Anti tumor activities of lentinan and micellapist in tumor-bearing mice. Gan To Kagaku Ryoho 33(12): 1726–1729 (in Chinese).Google ScholarPubMed
Ni, Y, Su, Q and Liu, X (1998) Optimization test on water extraction process for radix astragali. Chinese Pharmaceutical Journal 23(5): 284–286 (in Chinese).Google Scholar
Ooi, LSM, Ooi, VEC and Fung, MC (2002) Induction of gene expression of immunomodulatory cytokines in the mouse by polysaccharides from Ganoderma lucidum (Curt.: Fr.) P. Karst (Aphyllophoromycetideae). International Journal of Medicinal Mushrooms 4: 27–35.CrossRefGoogle Scholar
Raa, J (2000) The use of immune-stimulants in fish and shellfish feeds. In: Cruz-Suárez, LE., Ricque-Marie, D., Tapia-Salazar, M., Olvera-Novoa, MA and Civera-Cerecedo, R. (editors). Advance en Nutricion Acouicola V. Memorias del V Simposium Internacional de Nutricion Acouicola, 19–22 November, Merida, Yucatan, Mexico, pp. 47–56.Google Scholar
Sakai, M (1999) Current research status of fish immunostimulants. Aquaculture 172(1): 63–92.CrossRefGoogle Scholar
Wang, WB, Wang, JG, Li, AH and Lin, XT (2006) A brief introduction on the application of Chinese herbs in agriculture. Journal of China Fisheries 7: 60–61.Google Scholar
Yin, GJ, Geert, W and Li, YM (2004) Effect of Astragalus radix on proliferation and nitric oxide production of head kidney macrophages in Cyprinus carpio: an in vitro study. Journal of Fisheries of China 28(6): 628–632.Google Scholar
Young, SL, Kyung, OK and Chan, WP (2005) Pro-inflammatory cytokine gene expression and nitric oxide regulation of aqueous extracted Astragalus radix in RAW 264.7 macrophage cells. Journal of Ethnopharmacology 100: 289–294.Google Scholar
Zhang, FM, Zhang, SQ, Sun, F, Liu, ZY and Chen, XQ (2006) Prohibition of lentinan on influenza viruses. Journal of Changchun University of Traditional Chinese Medicine 22(4): 11–12 (in Chinese).Google Scholar
Zhang, T, Liu, ZY, Wei, FX and Zhang, T (2003) Effects of lentinus on immunologic function in mice. Heilongjiang Medicine and Pharmacy 26(6): 108–109 (in Chinese).Google Scholar
Zlotnik, A and Yoshie, O (2000) Chemokines: a new classification system and their role in immunity. Immunity 12(2): 121–127.CrossRefGoogle ScholarPubMed