Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-11-23T22:50:50.958Z Has data issue: false hasContentIssue false
  • English
  • Français

Improving biocontrol effect of Pseudomonas fluorescens P5 on plant diseases by genetic modification with chitinase gene

Published online by Cambridge University Press:  12 February 2007

Xu Xiao-Jing ,
Zhang Li-Qun ,
Zhu You-Yong  and
Tang Wen-Hua
Xu Xiao-Jing
Affiliation:
Department of Plant Pathology, China Agricultural University, Beijing 100094, China
Zhang Li-Qun*
Affiliation:
Department of Plant Pathology, China Agricultural University, Beijing 100094, China
Zhu You-Yong
Affiliation:
Key Laboratory for Plant Pathology of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China
Tang Wen-Hua
Affiliation:
Department of Plant Pathology, China Agricultural University, Beijing 100094, China
*
*Corresponding author: Email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Pseudomonas fluorescens P5 was isolated in Yunnan Province and proved to be an effective biocontrol agent of various soil-borne plant diseases. The aim of the study was to enhance the biocontrol effect of this strain by introducing a chitinase gene. A 6.5 kb DNA fragment containing the chitinase gene chiB was inserted into pDSK519 vector to construct a new plasmid, pDSK51965. This plasmid was introduced into P. fluorescens P5 and resulted in a new strain named P5-1. Restriction enzyme digestion and chitin plate culture confirmed that P5-1 contained a functional chitinase gene. Compared with wild-type P5 in pot experiments, P5-1 had an increased effect against rice sheath blight and cotton damping-off caused by Rhizoctonia solani. It also increased the effect on suppression of wheat take-all caused by Gaeumannomyces graminis var. tritici.

Keywords

Pseudomonaschitinase genewheat take-allrice sheath blightcotton damping-off
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 2 , Issue 1 , April 2005 , pp. 23 - 27
Copyright
Copyright © China Agricultural University and Cambridge University Press 2005

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bolar, JP, Norelli, JL, Wong, KW, Hayes, CK, Harman, GE and Aldwinckle, HS (2000) Expression of endochitinase from Trichoderma harzianum in transgenic apple increases resistance to apple scab and reduced vigor. Phytopathology 90: 72–77.CrossRefGoogle Scholar
Chet, I, Ordentlich, A, Shapira, R and Oppenheim, A (1990) Mechanisms of biocontrol of soil-borne plant pathogens by rhizobacteria. Plant and Soil 129: 85–92.CrossRefGoogle Scholar
Cook, RJ (1993) Making greater use of introduced microorganisms for biological control of plant pathogens. Annual Review of Phytopathology 31: 53–80.CrossRefGoogle ScholarPubMed
Fuchs, RL, Mopherson, SA and Drahos, DJ (1986) Cloning a Serratia marcescens gene encoding chitinase. Applied and Environmental Microbiology 51: 504–509.CrossRefGoogle ScholarPubMed
Gupta, CP, Dubey, RC, Kang, SC and Maheshwari, DK (2001) Antibiosis-mediated necrotrophic effect of Pseudomonas GRC2 against two fungal plant pathogens. Current Science 81: 91–94.Google Scholar
He, LY (1985) Research progress of bacteria as biological control agent on plant disease. Biological Control Bulletin 1 (3): 28–31 (in Chinese).Google Scholar
Irani, VR and Rowe, JJ (1997) Enhancement of transformation in Pseudomonas aeruginosa PAO1 by Mg 2+ and heat. Biological Techniques 22: 54–56.Google Scholar
Keen, NT, Tamaki, S, Kobayashi, D and Trollinger, D (1988) Improved broad-host-range plasmids for DNA cloning in Gram-negative bacteria. Gene 70: 191–197.CrossRefGoogle ScholarPubMed
Leong, J (1986) Siderophores; their biochemistry and possible role in the biocontrol of plant pathogens. Annual Review of Phytopathology 24: 187–209.CrossRefGoogle Scholar
Maurhofer, M, Keel, C, Haas, D, Défago, G (1995) Influence of plant species on disease suppression by Pseudomonas fluorescens strain CHA0 with enhanced antibiotic production. Plant Pathology 44: 40–50.CrossRefGoogle Scholar
Ordentlich, A, Elad, Y and Chet, I (1998) The role of chitinase of Serratia marcescens in biocontrol of Sclerotium rolfsii. Phytopathology 78: 84–88.Google Scholar
O'Sullivan, DJ, O'Gara, F (1992) Traits of fluorescent Pseudomonas spp. involved in suppression of plant root pathogens. Microbiology and Molecular Microbiology Reviews 56: 662–676.CrossRefGoogle ScholarPubMed
Sambrook, J, Fritsch, EF and Maniatis, T (1989) Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.Google Scholar
Shapira, R, Ordentlich, A, Chet, I and Oppenheim, AB (1989) Control of plant diseases by chitinase expressed from cloned DNA in Escherichia coli. Phytopathology 79: 1246–1249.CrossRefGoogle Scholar
Simi, K (1994) The chitinase encoding T7-based chiA gene endows Pseudomonas fluorescence with the capacity to control plant pathogens in soil. Gene 147: 81–83.Google Scholar
Sundheim, L, Poplawsky, RA and Ellingboe, HA (1988) Molecular cloning of two chitinase genes from Serratia marcescens and their expression in Pseudomonas species. Physiological and Molecular Plant Pathology 33: 483–491.CrossRefGoogle Scholar
Wang, YM and Tang, WH (1998) Expression of chitinase and b-1,3-glucanase genes in Bacillus subtilis B-908. In: Research and Control of Plant Disease. Beijing: China Agricultural Science and Technology Press, pp. 423–426 (in Chinese).Google Scholar
Zhang, LQ and Tang, WH (1998) Subcloning of chitinase gene and transfer into Bacillus thuringiensis. In: Research and Control of Plant Disease. Beijing: China Agricultural Science and Technology Press, pp. 488–491 (in Chinese).Google Scholar