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Genetic diversity of pummelo (Citrus grandis Osbeck) and its relatives based on simple sequence repeat markers

Published online by Cambridge University Press:  12 February 2007

Liu Yong ,
Liu De-Chun ,
Wu Bo  and
Sun Zhong-Hai
Liu Yong
Affiliation:
Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China
Liu De-Chun
Affiliation:
College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China
Wu Bo
Affiliation:
College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China
Sun Zhong-Hai*
Affiliation:
Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China
*
*Corresponding author. Email: [email protected]
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Abstract

Genetic diversity in 122 accessions of pummelo (Citrus grandis Osbeck) and its related varieties was assessed using simple sequence repeat (SSR) markers. Thirty-one pairs of SSR informative primers generated a total of 335 alleles. The average number of alleles per locus was 9.85. The value of allelic polymorphism information content (PIC) ranged from 0.1939 to 0.9073, with an average of 0.7085 per primer. The 122 accessions of pummelo and its related varieties could be clustered into seven groups by the unweighted pair-group method arithmetic average (UPGMA), in which the 110 pummelo accessions could be divided into 18 subgroups at similarity coefficient of 0.712. These subgroups were mainly composed of the Shatian pummelo variety group, the Wendan variety group and many of the hybrid pummelo groups. The classification method can be used in targeting many varieties in order to widen the genetic background of pummelo.

Keywords

genetic diversitygermplasm resourcespummeloSSR markersUPGMA cluster analysis
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 3 , Issue 2 , August 2006 , pp. 119 - 126
Copyright
Copyright © China Agricultural University and Cambridge University Press 2006

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References

Ahmad, R, Struss, D and Southwick, SM (2003) Development and characterization of microsatellite markers in Citrus. Journal of the American Society for Horticultural Science 128(4): 584–590.Google Scholar
Cheng, YJ, Yi, HL, Pang, XM, Guo, W and Deng, XX (2001) An efficient method for genomic DNA extraction from woody fruit plants. Journal of Huazhong Agricultural University 20(5): 481–483 (in Chinese with English abstract).Google Scholar
Duan, YC, He, RC and Jiang, ZS (1991) The comparision of kargotype and Giemsa banding pattern based on Shatian pummelo and somatic variation accessions. Journal of Hunan Agricultural College 17(1): 49–53 (in Chinese with English abstract).Google Scholar
Fang, DQ and Roose, ML (1997) Identification of closely related citrus cultivars with inter-simple sequence repeat markers. Theoretical and Applied Genetics 95: 408–417.Google Scholar
Fang, DQ, Zhang, WC and Xiao, SY (1993) Classification and evolution study of Citrus by isozyme. Acta Phytotaxonomica Sinica 31(4): 329–352 (in Chinese with English abstract).Google Scholar
He, SW (1978) A preliminary study of the native Citrus in central China. Acta Horticulturae Sinica 6(1): 19–25 (in Chinese with English abstract).Google Scholar
He, TF (1999) Pummelo Cultivation in China. Beijing: China Agricultural Press (in Chinese).Google Scholar
Kijas, JMH, Thomas, MR, Fowler, JCS and Roose, ML (1997) Integration of trinucleotide microsatellite into a linkage map of Citrus. Theoretical and Applied Genetics 94: 701–708.Google Scholar
Nei, M and Li, WH (1979) Mathematical model for studing genetic variation in terms of restriction endonuclease. Proceedings of the National Academy of Sciences of the USA 76: 5269–5273.Google Scholar
Pomology Research institute, CAAS (1998) Germplasms Resource Catalogue of Fruit Trees. Beijing: China Agricultural Press (in Chinese).Google Scholar
Scarano, MT, Tusa, N, Abbate, L, Lucretti, S, Nardi, L and Ferrante, S (2003) Flow cytometry, SSR and modified AFLP markers for the identification of zygotic plantlets in backcrosses between ‘Femminello’ lemon cybrids (2 n and 4 n ) and a diploid clone of ‘Femminello’ lemon (Citrus limon L. Burm. F) tolerant to mal secco disease. Plant Science 164: 1009–1017.CrossRefGoogle Scholar
Smith, JSC, Chin, ECL, Shu, H, et al. (1997) An evaluation of the utility of SSR loci as molecular markers in maize: comparsions with data from RFLPs and pedigree. Theoretical and Applied Genetics 95: 163–173.Google Scholar
Zhang, TP, Peng, SL, Ling, DH, Li, D and Gan, LS (2001a) Analysis of genetic relationships among Shatianyou Pomelo lines by RAPD markers. Guihaia 21(3): 247–251 (in Chinese with English abstract).Google Scholar
Zhang, TP, Peng, SL, Wang, ZF, Ling, DH and Gan, LS (2001b) Genetic relationships among cultivars of Citrus maxima (Burm.) Merr. using RAPD marker technique. Journal of Tropical and Subtropical Botany 9(4): 322–328 (in Chinese with English abstract).Google Scholar
Zhebentyayeva, TN, Reighard, GL, Gorina, VM and Abbott, A (2003) Simple sequence repeat (SSR) analysis for assessment of genetic variability in apricot germplasm. Theoretical and Applied Genetics 106: 435–444.CrossRefGoogle ScholarPubMed
Zhong, GY and Ye, YM (1993) A numerical taxonomic study of Citrus and its close relatives. Acta Phytotaxonomica Sinica 31(3): 250–260 (in Chinese with English abstract).Google Scholar