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Genetic diversity of pummelo (Citrus grandis Osbeck) and its relatives based on simple sequence repeat markers

Published online by Cambridge University Press:  12 February 2007

Liu Yong ,
Liu De-Chun ,
Wu Bo  and
Sun Zhong-Hai
Liu Yong
Affiliation:
Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China
Liu De-Chun
Affiliation:
College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China
Wu Bo
Affiliation:
College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China
Sun Zhong-Hai*
Affiliation:
Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China
*
*Corresponding author. Email: [email protected]
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Abstract

Genetic diversity in 122 accessions of pummelo (Citrus grandis Osbeck) and its related varieties was assessed using simple sequence repeat (SSR) markers. Thirty-one pairs of SSR informative primers generated a total of 335 alleles. The average number of alleles per locus was 9.85. The value of allelic polymorphism information content (PIC) ranged from 0.1939 to 0.9073, with an average of 0.7085 per primer. The 122 accessions of pummelo and its related varieties could be clustered into seven groups by the unweighted pair-group method arithmetic average (UPGMA), in which the 110 pummelo accessions could be divided into 18 subgroups at similarity coefficient of 0.712. These subgroups were mainly composed of the Shatian pummelo variety group, the Wendan variety group and many of the hybrid pummelo groups. The classification method can be used in targeting many varieties in order to widen the genetic background of pummelo.

Keywords

genetic diversitygermplasm resourcespummeloSSR markersUPGMA cluster analysis
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 3 , Issue 2 , August 2006 , pp. 119 - 126
Copyright
Copyright © China Agricultural University and Cambridge University Press 2006

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