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Effect of nitric oxide on Sertoli cell microtubule of piglets

Published online by Cambridge University Press:  29 January 2010

Yang Li ,
Wang Xian-zhong ,
Yang Meng-bo  and
Zhang Jia-hua
Yang Li
Affiliation:
Chongqing Key Laboratory of Forage & Herbivore, College of Animal Science and Technology, Southwest University, Chongqing 400716, China
Wang Xian-zhong
Affiliation:
Chongqing Key Laboratory of Forage & Herbivore, College of Animal Science and Technology, Southwest University, Chongqing 400716, China
Yang Meng-bo
Affiliation:
Chongqing Key Laboratory of Forage & Herbivore, College of Animal Science and Technology, Southwest University, Chongqing 400716, China
Zhang Jia-hua*
Affiliation:
Chongqing Key Laboratory of Forage & Herbivore, College of Animal Science and Technology, Southwest University, Chongqing 400716, China
*
* Corresponding author. E-mail: [email protected]
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Abstract

To illustrate the effect of nitric oxide (NO) on the microtubules of Sertoli cells (SC), SCs of piglets were treated with sodium nitroprusside (SNP). Changes in cell viability, anti-oxidant activity, enzyme activity and p38 mutagen-activated protein kinase (p38MAPK) activation were detected. The results were as follows. A low concentration of NO can keep SC microtubule and cell viability normal, and a high concentration of NO could increase p38MAPK activation, decrease anti-oxidant activity and transferrin secretion, and destroy the structure and distribution of the microtubules. The results suggest that SNP treatment results in an increase in NO in SCs and decreased cell anti-oxidant activity. The high concentration of NO destroys cell microtubules by activating p38MAPK.

Keywords

Sertoli cellsnitric oxidemicrotubule
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 6 , Issue 3 , December 2009 , pp. 257 - 263
Copyright
Copyright © China Agricultural University 2009

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