Hostname: page-component-586b7cd67f-r5fsc Total loading time: 0 Render date: 2024-11-27T19:58:38.101Z Has data issue: false hasContentIssue false
  • English
  • Français

Drought-tolerant gene screening in wheat using rice microarray

Published online by Cambridge University Press:  27 June 2008

Xu Zhou-Da ,
Jing Rui-Lian ,
Gan Qiang ,
Zeng Hai-Pan ,
Sun Xue-Hui ,
Leung Hei ,
Lu Tie-Gang  and
Liu Guo-Zhen
Xu Zhou-Da
Affiliation:
College of Life Sciences, Agriculture University of Hebei, Baoding 071000, China
Jing Rui-Lian
Affiliation:
Chinese Academy of Agricultural Sciences, Beijing 100081, China
Gan Qiang
Affiliation:
Institute of Genetic and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
Zeng Hai-Pan
Affiliation:
Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101300, China
Sun Xue-Hui
Affiliation:
Chinese Academy of Agricultural Sciences, Beijing 100081, China
Leung Hei
Affiliation:
International Rice Research Institute, Philippines
Lu Tie-Gang
Affiliation:
Chinese Academy of Agricultural Sciences, Beijing 100081, China
Liu Guo-Zhen*
Affiliation:
College of Life Sciences, Agriculture University of Hebei, Baoding 071000, China Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101300, China
*
*Corresponding author. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

To investigate the wheat transcriptional profile under drought stress, a drought-tolerant variety of wheat (Triticum aestivum), Hanxuan 10, was treated with polyethylene glycol (PEG6000) and samples were collected at 0, 1, 6 and 24 h. Complementary DNA was labelled with fluorescent dye and hybridized with the BGI-RiceChip, a whole genome rice gene chip platform, which contains over 60 000 oligos based on the rice genome sequence. Data analysis detected 166, 207 and 328 differentially expressed genes (DGs), respectively, at 1, 6 and 24 h, indicating that the number of DGs increased with the length of the PEG treatment. Functional category analysis showed that the number of DGs related to energy metabolism pathways increased – 4.2%, 8.2% and 16.8%, respectively, as a proportion of the total number of DGs. Most of the photosynthesis-related genes were up-regulated. It is interesting to note that Psbr and ribulose-bisphosphate carboxylase (Rubisco)-coding genes were down-regulated, suggesting their potential role in the response to drought tolerance.

Keywords

wheatdrought tolerancemicroarraytranscription
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 1 , April 2008 , pp. 43 - 48
Copyright
Copyright © China Agricultural University 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

First published in Journal of Agricultural Biotechnology 2007, 15(5): 821–827

References

Anderson, JV and Davis, DG (2004) Abiotic stress alters transcript profiles and activity of glutathione S-transferase, glutathione peroxidase, and glutathione reductase in Euphorbia esula. Physiology Plant 120(3): 421–433.CrossRefGoogle ScholarPubMed
Bauwe, H and Kolukisaoglu, Ü (2003) Genetic manipulation of glycine decarboxylation. Journal of Experimental Botany 54(387): 1523–1535.CrossRefGoogle ScholarPubMed
Bukhov, N and Carpentier, R (2004) Alternative photosystem I-driven electron transport routes: mechanisms and functions. Photosynthesis Research 82(1): 17–33.CrossRefGoogle ScholarPubMed
Chaves, MM and Oliveira, MM (2004) Mechanisms underlying plant resilience to water deficits: prospects for water-saving agriculture. Journal of Experimental Botany 55: 2365–2384.CrossRefGoogle ScholarPubMed
Close, TJ (1996) Dehydrins: emergence of a biochemical role of a family of plant dehydration proteins. Physiologia Plantarum 97: 795–803.CrossRefGoogle Scholar
Collett, H, Butowt, R, Smith, J, Farrant, J and Illing, N (2003) Photosynthetic genes are differentially transcribed during the dehydration–rehydration cycle in the resurrection plant, Xerophyta humilis. Journal of Experimental Botany 54(392): 2593–2595.CrossRefGoogle ScholarPubMed
Esquivel, MG, Pinto, TS, Marin-Navarro, J and Moreno, J (2006) Substitution of tyrosine residues at the aromatic cluster around the betaA–betaB loop of rubisco small subunit affects the structural stability of the enzyme and the in vivo degradation under stress conditions. Biochemistry 45(18): 5745–5753.CrossRefGoogle ScholarPubMed
Goff, SA (1999) Rice as a model for cereal genomics. Current Opinion in Plant Biology 2: 86–89.CrossRefGoogle Scholar
Golding, AJ and Johnson, GN (2004) Down-regulation of linear and activation of cyclic electron transport during drought. Planta 218(4): 682.CrossRefGoogle Scholar
Haupt-Herting, S and Fock, HP (2002) Oxygen exchange in relation to carbon assimilation in water-stressed leaves during photosynthesis. Annals of Botany 89: 851–859.CrossRefGoogle ScholarPubMed
Ma, L, Chen, C, Liu, X, et al. (2005) A microarray analysis of the rice transcriptome and its comparison to Arabidopsis. Genome Research 15(9): 1274–1283.CrossRefGoogle ScholarPubMed
MacRae, E. (2007) Extraction of plant RNA. Methods in Molecular Biology 353: 15–24.Google ScholarPubMed
Pandurangam, V, Sharma-Natu, P, Sreekanth, B and Ghildiyal, MC (2006) Photosynthetic acclimation to elevated CO2 in relation to Rubisco gene expression in three C3 species. Indian Journal of Experimental Biology 44(5): 408–415.Google ScholarPubMed
Pieters, AJ and El Souki, S (2005) Effects of drought during grain filling on PS II activity in rice. Plant Physiology 162(8): 903–911.CrossRefGoogle ScholarPubMed
Pnueli, L, Hallak-Herr, E, Rozenberg, M, et al. (2002) Molecular and biochemical mechanisms associated with dormancy and drought tolerance in the desert legume Retama raetam. The Plant Journal 31(3): 319–330.CrossRefGoogle ScholarPubMed
Ramachandra Reddy, A, Chaitanya, KV and Vivekanandan, M (2004) Drought-induced responses of photosyphotosynthesis and antioxidant metabolism in higher plants. Plant Physiology 161(11): 1189–1202.CrossRefGoogle ScholarPubMed
Smyth, GK and Speed, TP (2003) Normalization of cDNA microarray data. Methods 31, 265–273.CrossRefGoogle ScholarPubMed
Suorsa, M, Sirpio, S, Allahverdiyeva, Y, et al. (2006) PsbR, a missing link in the assembly of the oxygen-evolving complex of plant photosystem II. Journal of Biology Chemistry 281(1): 145–150.CrossRefGoogle ScholarPubMed
Trouverie, J, Thévenot, C, Rocher, JP, Sotta, B and Prioul, JL (2003) The role of abscisic acid in the response of a specific vacuolar invertase to water stress in the adult maize leaf. Journal of Experimental Botany 54, 2177–2186.CrossRefGoogle ScholarPubMed
van Rensen, JJS and Curwiel, VB (2000) Multiple functions of photosystem II. Indian Journal of Biochemistry and Biophysics 37, 377–382.Google ScholarPubMed
Wang, W, Vinocur, B and Altman, A (2003) Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta 218(1): 1–14.CrossRefGoogle ScholarPubMed
Wingler, A, Lea, PJ, Quick, WP and Leegood, RC (2000) Photorespiration: metabolic pathways and their role in stress protection. Philosophical Transactions of the Royal Society of London, Series B 29: 355(1402): 1517–1529.CrossRefGoogle ScholarPubMed
Zhuang, QS (2003) Wheat Varieties Development and Genealogy Analysis in China. Beijing: China Agricultural Press, pp. 63–64. 118 (in Chinese).Google Scholar
Zou, J, Rodriguez-Zas, S, Aldea, M, et al. (2005) Expression profiling soybean response to Pseudomonas syringae reveals new defense-related genes and rapid HR-specific down-regulation of photosynthesis. Molecular Plant–Microbe Interactions 18(11): 1161–1174.CrossRefGoogle ScholarPubMed