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Drought-tolerant gene screening in wheat using rice microarray

Published online by Cambridge University Press:  27 June 2008

Xu Zhou-Da ,
Jing Rui-Lian ,
Gan Qiang ,
Zeng Hai-Pan ,
Sun Xue-Hui ,
Leung Hei ,
Lu Tie-Gang  and
Liu Guo-Zhen
Xu Zhou-Da
Affiliation:
College of Life Sciences, Agriculture University of Hebei, Baoding 071000, China
Jing Rui-Lian
Affiliation:
Chinese Academy of Agricultural Sciences, Beijing 100081, China
Gan Qiang
Affiliation:
Institute of Genetic and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
Zeng Hai-Pan
Affiliation:
Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101300, China
Sun Xue-Hui
Affiliation:
Chinese Academy of Agricultural Sciences, Beijing 100081, China
Leung Hei
Affiliation:
International Rice Research Institute, Philippines
Lu Tie-Gang
Affiliation:
Chinese Academy of Agricultural Sciences, Beijing 100081, China
Liu Guo-Zhen*
Affiliation:
College of Life Sciences, Agriculture University of Hebei, Baoding 071000, China Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101300, China
*
*Corresponding author. E-mail: [email protected]
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Abstract

To investigate the wheat transcriptional profile under drought stress, a drought-tolerant variety of wheat (Triticum aestivum), Hanxuan 10, was treated with polyethylene glycol (PEG6000) and samples were collected at 0, 1, 6 and 24 h. Complementary DNA was labelled with fluorescent dye and hybridized with the BGI-RiceChip, a whole genome rice gene chip platform, which contains over 60 000 oligos based on the rice genome sequence. Data analysis detected 166, 207 and 328 differentially expressed genes (DGs), respectively, at 1, 6 and 24 h, indicating that the number of DGs increased with the length of the PEG treatment. Functional category analysis showed that the number of DGs related to energy metabolism pathways increased – 4.2%, 8.2% and 16.8%, respectively, as a proportion of the total number of DGs. Most of the photosynthesis-related genes were up-regulated. It is interesting to note that Psbr and ribulose-bisphosphate carboxylase (Rubisco)-coding genes were down-regulated, suggesting their potential role in the response to drought tolerance.

Keywords

wheatdrought tolerancemicroarraytranscription
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 1 , April 2008 , pp. 43 - 48
Copyright
Copyright © China Agricultural University 2008

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Footnotes

First published in Journal of Agricultural Biotechnology 2007, 15(5): 821–827

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