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Diversity analysis of endophytic bacteria within Azolla microphylla using PCR-DGGE and electron microscopy

Published online by Cambridge University Press:  03 March 2009

Zheng Si-Ping ,
Chen Bin ,
Guan Xiong  and
Zheng Wei-Wen
Zheng Si-Ping
Affiliation:
Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Chen Bin
Affiliation:
Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China
Guan Xiong
Affiliation:
Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Zheng Wei-Wen*
Affiliation:
Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China
*
*Corresponding author. E-mail: [email protected]
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Abstract

Using 16S rDNA-polymerase chain reaction–denaturing gradient gel electrophoresis (PCR-DGGE), electron microscopy and a conventional plating method, the genetic diversity and phenotype polymorphism of the endophytic bacteria within Azolla microphylla were explored. The 16S rDNA-PCR-DGGE profile showed a complex and divergent bacterial community, with Bacillus cereus as the dominant species, within the Azolla–cyanobacteria association. This result was supported by the fact that endobacterial cells exhibited distinct ultrastructural characteristics in vivo and, in vitro, bacteria displayed various colonies with different sizes, shapes and colours. This study demonstrates that the genetic diversity of endophytic bacteria in Azolla can be investigated using the16S rDNA-PCR-DGGE technique.

Keywords

Azollaendophytic bacteriadiversity16S rDNA-PCR-DGGE
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 3 , December 2008 , pp. 269 - 276
Copyright
Copyright © China Agricultural University 2008

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Footnotes

First published in Journal of Agricultural Biotechnology 2008, 16(3): 508–514

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