Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-11-24T03:08:29.070Z Has data issue: false hasContentIssue false
  • English
  • Français

Development of AFLP markers linked to stem nematode resistance gene in sweet potato (Ipomoea batatas (L.) Lam.)

Published online by Cambridge University Press:  30 October 2009

Jie Qin ,
Li Hua ,
Zhai Hong ,
Wang Yu-Ping ,
Li Qiang ,
Ma Dai-Fu ,
Xie Yi-Ping  and
Liu Qing-Chang
Jie Qin
Affiliation:
Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture, China Agricultural University, Beijing 100193 Beijing Key Laboratory of Crop Genetic Improvement, Beijing 100193 Langfang Academy of Agriculture and Forestry Science, Langfang 065000, China
Li Hua
Affiliation:
Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture, China Agricultural University, Beijing 100193 Beijing Key Laboratory of Crop Genetic Improvement, Beijing 100193
Zhai Hong
Affiliation:
Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture, China Agricultural University, Beijing 100193 Beijing Key Laboratory of Crop Genetic Improvement, Beijing 100193 Key Laboratory of Crop Heterosis and Utilization, Ministry of Education, Beijing 100193
Wang Yu-Ping
Affiliation:
Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture, China Agricultural University, Beijing 100193 Beijing Key Laboratory of Crop Genetic Improvement, Beijing 100193 Key Laboratory of Crop Heterosis and Utilization, Ministry of Education, Beijing 100193
Li Qiang
Affiliation:
Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture, China Agricultural University, Beijing 100193 Xuzhou Sweet Potato Research Center, Xuzhou 221121
Ma Dai-Fu
Affiliation:
Xuzhou Sweet Potato Research Center, Xuzhou 221121
Xie Yi-Ping
Affiliation:
Xuzhou Sweet Potato Research Center, Xuzhou 221121
Liu Qing-Chang*
Affiliation:
Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture, China Agricultural University, Beijing 100193 Beijing Key Laboratory of Crop Genetic Improvement, Beijing 100193 Key Laboratory of Crop Heterosis and Utilization, Ministry of Education, Beijing 100193
*
*Corresponding author. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Amplified fragment length polymorphism (AFLP) markers linked to the stem nematode resistance gene were developed in sweet potato (Ipomoea batatas (L.) Lam.). Using bulked segregant analysis (BSA), 800 AFLP primer combinations were screened in the resistant and susceptible bulked DNA from the 186 progeny of an F1 single-cross population of Xu781 (resistant parent)×Xushu18 (susceptible parent), and 245 of these AFLP primers showed polymorphic bands between resistant and susceptible DNA. Primer combinations detecting polymorphism between the two bulks were used to screen the parents and eight individuals from each of the bulks. The results showed that E2M23 and E33M20 produced a specific band of about 500 bp and 200 bp in length, respectively, in the resistant plants but not in the susceptible plants, suggesting that the markers named E2M23500 and E33M20200 linked to a gene for stem nematode resistance. Amplified analysis of the 186 F1 individuals indicated that the genetic distance between these two markers and the stem nematode resistance gene was 6.9 cM and 11.1 cM, respectively, measured with Mapmaker 3.0. These two AFLP markers were used to identify ten sweet potato varieties planted widely in China and the results were consistent with those of conventional resistance identification, indicating that the two markers can be used in molecular marker-assisted breeding for stem nematode resistance in the sweet potato.

Keywords

sweet potato (Ipomoea batatas (L.) Lam.)stem nematodeAFLP marker
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 6 , Issue 2 , August 2009 , pp. 97 - 101
Copyright
Copyright © China Agricultural University 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

He, XQ, Liu, QC, Ishiki, K, Zhai, H and Wang, YP (2006) Genetic diversity and genetic relationships among Chinese sweet potato landraces revealed by RAPD and AFLP markers. Breeding Science 56(2): 201–207.Google Scholar
Jiang, L, Yuan, L, Zha, XD, et al. (2007) A RAPD marker linked to the resistance Ditylenchus destructor in sweet potato. Molecular Plant Breeding 5(5): 655–660 (in Chinese with English abstract).Google Scholar
Kriegner, A, Cervantes, JC, Burg, K, Mwanga, ROM and Zhang, DP (2003) A genetic linkage map of sweet potato (Ipomoea batatas (L.) Lam.) based on AFLP markers. Molecular Breeding 11: 169–185.Google Scholar
Li, Q, Jie, Q, Liu, QC, et al. (2007) An efficient and rapid method for sweet potato genomic DNA extraction. Molecular Plant Breeding 5(5): 743–746 (in Chinese with English abstract).Google Scholar
Ma, DF, Li, HM, Xie, YP, Li, XY, Zhu, CW and Jiang, XM (1997) Breeding of stem-nematode resistant varieties in sweet potato. Crops 2: 15–16 (in Chinese with English abstract).Google Scholar
Mcharo, M, LaBonte, DR, Clark, C, Hoy, M and Oard, JH (2005) Molecular marker variability for southern root-knot nematode resistance in sweet potato. Euphytica 144: 125–132.Google Scholar
Michelmore, RW, Paran, I and Kesseli, RV (1991) Identification of markers linked to disease resistance genes by bulked segregating populations. Proceedings of the National Academy of Sciences, USA 88: 9828–9832.Google Scholar
Ukoskit, K, Thompson, PG, Watson, CE Jr and Lawrence, GW (1997) Identifying a randomly amplified polymorphic DNA (RAPD) marker linked to a gene for root knot nematode resistance in sweet potato. Journal of American Society Horticultural Science 122: 818–822.Google Scholar
Vos, P, Hogers, R, Bleeker, M, Reijans, M, Van de Lee, T and Hornes, M (1995) AFLP, a new technique for DNA fingerprinting. Nucleic Acids Research 23(21): 4407–4414.Google Scholar
Xie, YP, Ma, DF, Li, HM, Li, XY, Li, Q and Ma, F (2002) Identification methods and evaluation of sweet potato resistance to Ditylenchus dipsaci (Kühn) Filipjev. Rain Fed Crops 22: 50–51 (in Chinese).Google Scholar
Xie, YZ, Yin, QH, Dai, QW and Qiu, RL (2004) Inheritance and breeding for resistance to sweet potato nematodes. Journal of Plant Genetic Resources 5(4): 393–396 (in Chinese with English abstract).Google Scholar
Zhang, DP, Rossel, G, Kriegner, A and Hijmans, R (1994) AFLP assessment of diversity in sweet potato from Latin America and the Pacific region: Its implications on the dispersal of the crop. Genetic Resources and Crop Evolution 45(3): 165–173.Google Scholar
Zhou, Z, Wang, X, Ma, DF, Li, HM, Xie, YP and Li, XY (2005) Identification of RAPD markers linked to stem-nematode resistant gene in sweet potato. Journal of Agricultural Biotechnology 13(5): 549–552 (in Chinese with English abstract).Google Scholar