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Classification of wheat PPO genes and effect of non-synonymous cSNP on kernel PPO activity

Published online by Cambridge University Press:  27 June 2008

Wang Xiao-Bo ,
Ma Chuan-Xi ,
Si Hong-Qi  and
He Xian-Fang
Wang Xiao-Bo
Affiliation:
Agronomy School of Anhui Agricultural University, Hefei 230036, China
Ma Chuan-Xi*
Affiliation:
Agronomy School of Anhui Agricultural University, Hefei 230036, China
Si Hong-Qi
Affiliation:
Agronomy School of Anhui Agricultural University, Hefei 230036, China
He Xian-Fang
Affiliation:
Agronomy School of Anhui Agricultural University, Hefei 230036, China
*
*Corresponding author. E-mail: [email protected]
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Abstract

Polyphenol oxidase (PPO) activity is highly related to the undesirable browning of wheat-based end products. In this study, wheat PPO sequences (mRNA) were searched/BLASTed in the NCBI database and aligned using DNAMAN software. The results showed that wheat PPO genes could be divided into two clusters (I and II) and that three genes (‘i’) of cluster II seemed not to be located on chromosomes 2A and 2D. Ninety-four single nucleotide polymorphisms (SNPs) were detected between two haplotypes of the PPO gene on chromosome 2D. Eighty of these were found in the coding region (coding (c) SNPs) and 36 were non-synonymous cSNPs, which could affect the PPO amino acid sequence. Primers (STS-H) were designed at some non-synonymous cSNPs sites and were used to investigate the correlations between allelic variants and PPO activity of seeds – a total of 130 common wheat varieties were evaluated in 2 years. The results showed that STS-H could amplify a 460 bp DNA fragment in most cultivars with high PPO activity, while no PCR product was detected in most cultivars with low PPO activity. To improve the selection efficiency of a single dominance molecular marker, the multiplex polymerase chain reaction (PCR) system of STS-H and STS01 markers was also studied, based on the complementary between them.

Keywords

wheat (Triticum aestivum)genepolyphenol oxidasemolecular markermultiplex PCR
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 1 , April 2008 , pp. 81 - 86
Copyright
Copyright © China Agricultural University 2008

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Footnotes

First published in Journal of Agricultural Biotechnology 2007, 15(6): 970–975

References

Anderson, JV and Morris, CF (2001) An improved whole-seed assay for screening wheat germplasm for polyphenol oxidase activity. Crop Science 41: 1697–1705.CrossRefGoogle Scholar
Baik, BK, Czuchajowska, Z and Pomeranz, Y (1995) Discoloration of dough for oriental noodles. Cereal Chemistry 72: 198–205.Google Scholar
Bhattacharya, M, Luo, Q and Corke, H (1999) Time-dependent changes in dough color in hexaploid wheat landraces differing in polyphenol oxidase activity. Agricultural Food Chemistry 47: 3579–3585.CrossRefGoogle ScholarPubMed
Demeke, T, Morris, CF and Campbell, KG (2001) Wheat analytical polyphenol oxidases distribution and genetic mapping in three inbred line populations. Crop Science 41: 1750–1757.CrossRefGoogle Scholar
Hagmann, M (1999) Human genome – a good SNP may be hard to find. Science 285: 21–22.CrossRefGoogle ScholarPubMed
Han, J, Ma, CX, Si, HQ, Han, LD and Chen, J (2006) The genetic analysis and variation of PPO activity in the early generation of yangmai 158×huaimai 18. Journal of Triticeae Crops 26(5): 38–41.Google Scholar
He, XY, He, ZH, Zhang, LP, et al. (2007) Allelic variation of polyphenol oxidase (PPO) genes located on chromosomes 2A and 2D and development of functional markers for the PPO genes in common wheat. Theoretical and Applied Genetics 115: 47–58.CrossRefGoogle ScholarPubMed
Hou, G (2001) Oriental noodles. Advances in Food and Nutrition Research 43: 142–194.Google ScholarPubMed
Jukanti, AK, Bruckner, PL and Fischer, AM (2004) Evaluation of wheat polyphenol oxidase genes. Cereal Chemistry 81(4): 481–485.CrossRefGoogle Scholar
Mares, DJ and Campbell, AW (2001) Mapping components of flour and noodle colour in Australian wheat. Australian Journal of Agricultural Research 52: 1297–1309.CrossRefGoogle Scholar
Morris, CF, Jeffers, HC and Engle, DE (2000) Effect of processing, formula and measurement variables on alkaline noodle color – toward an optimized laboratory system. Cereal Chemistry 77: 77–85.CrossRefGoogle Scholar
Park, WJ, Shelton, DR, Peterson, CJ, Martin, TJ, Kachman, SD and Wehling, RL (1997) Variation in polyphenol oxidase activity and quality characteristics among hard white wheat and hard red winter wheat samples. Cereal Chemistry 74: 7–11.CrossRefGoogle Scholar
Sun, DJ, He, ZH, Xia, XC, et al. (2005) A novel STS marker for polyphenol oxidase activity in bread wheat. Molecular Breeding 16: 209–218.CrossRefGoogle Scholar
Tenaillon, M, Sawkins, MC and Long, AD (2001) Pattern of DNA sequence polymorphism along chromosome l of maize. Proceedings of the National Academy of Sciences of the USA 98: 9161–9166.CrossRefGoogle ScholarPubMed
Wang, XB, Ma, C and Si, HQ (2006) Triticum aestivum polyphenol oxidase (PPO) gene: GenBank Accession Number DQ992480–DQ992483. Available at http://www.ncbi.nlm.nih.gov/Google Scholar
Zhang, LP, Ge, XX, He, ZH, et al. (2005) Mapping QTLs for polyphenol oxidase activity in DH population from commom wheat. Acta Agronomica Sinica 31(1): 7–10.Google Scholar
Zhou, YQ (2005) The Application of DNA Molecular Markers in Plants. Beijing: Chemical Industry Press.Google Scholar