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Biological characteristics of recombinant enhancin of Trichoplusia ni granulovirus expressed in Escherichia coli

Published online by Cambridge University Press:  20 March 2007

Yuan Zhe-Ming ,
Meng Xiao-Lin  and
Liu Shu-Sheng
Yuan Zhe-Ming
Affiliation:
Bio-safety Science and Technology College, Hunan Agricultural University, Changsha 410128, China
Meng Xiao-Lin*
Affiliation:
Institute of Virology, Wuhan University, Wuhan 430072, China
Liu Shu-Sheng
Affiliation:
Institute of Applied Entomology, Zhejiang University, Hangzhou 310029, China
*
*Corresponding author. E-mail: [email protected]
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Abstract

A recombinant enhancin from Trichoplusia ni granulovirus, expressed in Escherichia coli and named P96, had been shown in a previous study to increase significantly the efficacy of bio-insecticides applied to insect pests. Here, several biological characteristics were clarified to provide further proof for the activities of P96. The inclusion bodies formed by P96 were quite stable when expressed in E. coli; however, they could be effectively dissolved in sodium carbonate (Na2CO3) solution or in the midgut digestive secretion of the fifth-instar larvae of the cotton bollworm, Helicoverpa armigera, in vitro. According to sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE), the peritrophic membrane of the cotton bollworm was clearly decomposed by P96. With the increase of P96 concentration, the mortality of the cotton bollworm caused by nuclear polyhedrosis virus increased up to its saturation concentration, 6.76×105 inclusion bodies/ml. The bioactivity of P96 was strongly inhibited by ethylenediaminetetraacetic acid (EDTA), which formed a complex compound with the metal ion (Zn2+) in the protein.

Keywords

biological charactersHelicoverpa armigerarecombinant enhancinTrichoplusia ni granulovirus
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 3 , Issue 3 , December 2006 , pp. 171 - 176
Copyright
China Agricultural University and Cambridge University Press 2006

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