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Bacillus thuringiensis crystal protein toxicity against plant-parasitic nematodes

Published online by Cambridge University Press:  27 June 2008

Yu Zi-Quan ,
Wang Qian-Lan ,
Liu Bin ,
Zou Xue ,
Yu Zi-Niu  and
Sun Ming
Yu Zi-Quan
Affiliation:
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Wang Qian-Lan
Affiliation:
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Liu Bin
Affiliation:
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Zou Xue
Affiliation:
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Yu Zi-Niu
Affiliation:
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Sun Ming*
Affiliation:
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
*
*Corresponding author. E-mail: [email protected]
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Abstract

A bioassay method was developed to use the parasporal crystal protein of Bacillus thuringiensis against plant-parasitic nematodes. Using this method, the parasporal crystal proteins of ten Bt strains showed activity against plant-parasitic nematodes. The toxicity of YBT-021 against Meloidogyne hapla, Pratylenchus scribneri, Tylenchorhynchus sp., Ditylenchus destructor and Aphelenchoides sp. was also assayed. The resulting LC50 values were 35.62 μg/ml, 75.65 μg/ml, 94.31 μg/ml, 215.21 μg/ml and 128.76 μg/ml, respectively.

Keywords

Bacillus thuringiensisparasporal crystal proteinbioassayplant-parasitic nematodes
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 1 , April 2008 , pp. 13 - 17
Copyright
Copyright © China Agricultural University 2008

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Footnotes

First published in Journal of Agricultural Biotechnology 2007, 15(5): 867–871

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