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Assessing genetic diversity in three wild Brachymystax lenok populations using AFLP markers

Published online by Cambridge University Press:  29 January 2010

Wang Di ,
Li Shao-Wu ,
Xu Ge-Feng ,
Liu Yang ,
Mou Zhen-Bo  and
Lu Tong-Yan
Wang Di
Affiliation:
Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Science, Harbin 150070, China
Li Shao-Wu
Affiliation:
Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Science, Harbin 150070, China
Xu Ge-Feng
Affiliation:
Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Science, Harbin 150070, China
Liu Yang
Affiliation:
Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Science, Harbin 150070, China
Mou Zhen-Bo*
Affiliation:
Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Science, Harbin 150070, China
Lu Tong-Yan*
Affiliation:
Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Science, Harbin 150070, China
*
*Corresponding author. E-mail: [email protected] or [email protected]
*Corresponding author. E-mail: [email protected] or [email protected]
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Abstract

The genetic diversities of 72 individuals from three wild Lenok populations of Mudanjiang River (MD), Yalujiang River (YL) and Wusulijiang River (WSL) in the northeast of China were analysed using amplified fragment length polymorphism (AFLP) markers. The results showed that 541 polymorphic loci out of 559 were amplified by 12 primer pairs and the percentage of polymorphic loci was 96.78%. Shannon indices for the MD, YL and WSL populations were 0.3988±0.2913, 0.3254±0.3037, 0.2125±0.2862, respectively, and Nei's gene diversity indices were 0.2737±0.2062, 0.2229±0.2129, 0.1446±0.1985, respectively. The average total genetic diversity (Ht) was 0.3512±0.0.0208 and the average genetic diversity within populations (Hs) was 0.2137±0.0152. Among the three populations, the average genetic distance (Dst) was 0.1375 and the gene differentiation coefficient (Gst) was 0.3914. The genetic diversity was 60.85% within populations and 39.15% among populations. The gene flow index (Nm) was 0.7776. The analysis of molecular variance (AMOVA) indicated that the average fixation index (Fst) was 0.55336. The variance was 55.16% within populations and 44.84% among populations. The highest polymorphism ratio was in the MD group and the lowest in the WSL group.

Keywords

LenokAFLPgenetic diversity
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 6 , Issue 3 , December 2009 , pp. 241 - 247
Copyright
Copyright © China Agricultural University 2009

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