Hostname: page-component-cd9895bd7-q99xh Total loading time: 0 Render date: 2024-12-28T11:04:38.625Z Has data issue: false hasContentIssue false
  • English
  • Français

Analysis of restriction sites and Southern blotting of two molecular markers linked to grape seedless gene

Published online by Cambridge University Press:  12 February 2007

Yang Ke-Qiang ,
Wang Yue-Jin ,
Zhang Jin-Jin ,
Wang Xi-Ping ,
Wan Yi-Zhen  and
Zhang Jian-Xia
Yang Ke-Qiang
Affiliation:
Key Laboratory of Ministry of Agriculture of Northwest Horticulture Plant Germplasm and Genetic Improvement, College of Horticulture, Northwest A&F University, Yangling 712100, China College of Forestry, Shandong Agricultural University, Taian 271018, China
Wang Yue-Jin*
Affiliation:
Key Laboratory of Ministry of Agriculture of Northwest Horticulture Plant Germplasm and Genetic Improvement, College of Horticulture, Northwest A&F University, Yangling 712100, China
Zhang Jin-Jin
Affiliation:
Key Laboratory of Ministry of Agriculture of Northwest Horticulture Plant Germplasm and Genetic Improvement, College of Horticulture, Northwest A&F University, Yangling 712100, China College of Life Sciences, Shaanxi Normal University, Xi'an 710062, China
Wang Xi-Ping
Affiliation:
Key Laboratory of Ministry of Agriculture of Northwest Horticulture Plant Germplasm and Genetic Improvement, College of Horticulture, Northwest A&F University, Yangling 712100, China
Wan Yi-Zhen
Affiliation:
Key Laboratory of Ministry of Agriculture of Northwest Horticulture Plant Germplasm and Genetic Improvement, College of Horticulture, Northwest A&F University, Yangling 712100, China
Zhang Jian-Xia
Affiliation:
Key Laboratory of Ministry of Agriculture of Northwest Horticulture Plant Germplasm and Genetic Improvement, College of Horticulture, Northwest A&F University, Yangling 712100, China
*
*Corresponding author: Email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The cloning and sequencing of two specific molecular markers linked to grape (Vitis vinifera) seedless gene indicated that the full lengths of the markers, 39970524-5-564 (GenBank accession number: AY327513) and 39970524-6-1538 (GenBank accession number: AY327514), were 564 bp and 1538 bp, respectively. The restriction sites of sequences 39970524-5-564 and 39970524-6-1538 were analysed with Wingene231 software. The results indicated that there were 29 and 130 restriction sites in 39970524-5-564 and 39970524-6-1538, respectively, digested by restriction enzymes with recognition sites of six or more than six bases. There was no cleavage site in 39970524-5-564 and there was one site at 135 bp of 39970524-6-1538 when these two markers were digested by EcoRI. Both markers can not be digested by HindIII. Therefore, the full length of 39970524-5-564 and of the two bands of 39970524-6-1538 (1400 bp and 135 bp) could be cut out from the pGEMR-T Easy Vector by EcoRI. The labelled DNA of 39970524-5-564, used as a probe for Southern blotting with genomic DNA from Red Globe, Flame Seedless, Thompson Seedless, four seedless hybrids and three seeded hybrids, showed a unique hybrid band present in all seedless individuals, but absent in all seeded individuals. It was further indicated that the specific marker 39970524-5-564 was derived from the genome of seedless grapes.

Keywords

grape (Vitis vinifera)restriction sitesseedless geneSouthern blotting
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 3 , Issue 1 , April 2006 , pp. 13 - 17
Copyright
Copyright © China Agricultural University and Cambridge University Press 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Clark, MS (1998) Plant Molecular Biotechnology–Experimental Manual (translated by Gu HY and Ju LJ). Beijing: High Education Press, pp. 13–42 (in Chinese).Google Scholar
Lahogue, F, This, P and Bouquet, A (1998) Identification of a codominant SCAR marker linked to the seedlessness character in grapevine. Theoretical and Applied Genetics 97: 950–959.CrossRefGoogle Scholar
Li, DB and Xu, P (1994) Method and theory of recombinant DNA. Hangzhou: Science and Technology Press, pp. 69–83 (in Chinese).Google Scholar
Striem, MJ, Ben-Hayyim, G and Spiegel, RP (1996) Identifying molecular genetic markers associated with seedlessness in grape. Journal of the American Society of Horticultural Science 121: 758–763.CrossRefGoogle Scholar
Wang, YJ and Lamikanra, O (1997) Analysis of sequencing the RAPD marker linked to seedless gene in grape. Journal of Northwest Agricultural University 25(4): 1–5 (in Chinese with English abstract).Google Scholar
Wang, YJ and Lamikanra, O (2002) Application and synthesis on the DNA probe for detecting seedless genes in grape vine. Journal of Northwest Sci-tech University of Agricultural and Forestry (Natural Science Edition) 30(3): 42–46 (in Chinese with English abstract).Google Scholar
Wang, YJ, Lamikanra, O, Lu, J and Ramming, DW (1996) Identification of genetic marker linked to seedless genes in grapes using RAPD. Journal of Northwest Agricultural University 24(5): 1–10 (in Chinese with English abstract).Google Scholar
Wang, YJ, Lamikanra, O and Schell, L (1997) Identification of hybrids derived from Vitis rotundifolia and V. vinifera by RAPD analysis. Journal of Northwest Agricultural University 25(3): 16–20 (in Chinese with English abstract).Google Scholar