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An association between tyrosinase gene single nucleotide polymorphisms and melanin distribution in chicken

Published online by Cambridge University Press:  12 February 2007

Chen Zhi-Qiang
Affiliation:
National Key Laboratory for Agro-biotechnology and College of Animal Science and Technology, China Agricultural University, Beijing 100094, China Inner Mongolia Caoyuan Xingfa Co. Ltd, Chifeng 024076, China
Deng Xue-Mei
Affiliation:
National Key Laboratory for Agro-biotechnology and College of Animal Science and Technology, China Agricultural University, Beijing 100094, China
Zhou Jun
Affiliation:
National Key Laboratory for Agro-biotechnology and College of Animal Science and Technology, China Agricultural University, Beijing 100094, China
Li Ning*
Affiliation:
National Key Laboratory for Agro-biotechnology and College of Animal Science and Technology, China Agricultural University, Beijing 100094, China
Wu Chang-Xin
Affiliation:
National Key Laboratory for Agro-biotechnology and College of Animal Science and Technology, China Agricultural University, Beijing 100094, China
*
*Corresponding author: Email [email protected]

Abstract

Tyrosinase (TYR) is a key enzyme of melanin biosynthesis. Single-strand conformation polymorphism (SSCP) analysis was applied to detect the single nucleotide polymorphisms (SNPs) in the upstream regulating region from -641 to -2125 bp of the TYR gene. Three SNPs were found in this region. Correlations were obtained between genotypes of the SNP sites and pigment traits in chicken parental and F2 generations of the Chinese Agricultural University (CAU) resource population. The chi-square test indicated that these mutations were significantly related to shank and body skin colours.

Type
Research Article
Copyright
Copyright © China Agricultural University and Cambridge University Press 2005

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