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AFLP fingerprinting analysis of genetic polymorphism of 12 indigenous chicken breeds

Published online by Cambridge University Press:  15 June 2007

Gao Yu-Shi ,
Tu Yun-Jie ,
Tong Hai-Bing ,
Wang Ke-Hua  and
Chen Kuan-Wei
Gao Yu-Shi*
Affiliation:
Institute of Poultry, Academy of Agriculture Sciences in China, Yangzhou 225003, Jiangsu, China
Tu Yun-Jie
Affiliation:
Institute of Poultry, Academy of Agriculture Sciences in China, Yangzhou 225003, Jiangsu, China
Tong Hai-Bing
Affiliation:
Institute of Poultry, Academy of Agriculture Sciences in China, Yangzhou 225003, Jiangsu, China
Wang Ke-Hua
Affiliation:
Institute of Poultry, Academy of Agriculture Sciences in China, Yangzhou 225003, Jiangsu, China
Chen Kuan-Wei
Affiliation:
Institute of Poultry, Academy of Agriculture Sciences in China, Yangzhou 225003, Jiangsu, China
*
*Corresponding author. E-mail: [email protected]
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Abstract

A total of six amplified fragment length polymorphism (AFLP) primer combinations were used to detect genetic variation of pooled DNA in a sample of 12 chicken breeds indigenous to China, and AFLP DNA fingerprinting of each chicken breed was constructed. Polymorphic bands, specific bands and genetic similarity coefficients of 12 chicken breeds were calculated from AFLP data. A total of 279 polymorphic bands were generated by the six primer combinations, giving, on average, 46.5 polymorphic markers detected per primer combination. Nine specific bands were produced in the pooled DNA of Jiuyuan black and Dongxiang black chickens. However, one specific band was produced in the pooled DNA of Wenchang and Xingyi bantam chickens. An unweighted-pair-group method using average linkages (UPGMA) cluster analysis revealed that the 12 chicken breeds could be divided into three groups. Genetic similarity coefficients and the UPGMA tree of the 12 chicken breeds were consistent with their breeding history as well as their geographical distribution. Based on AFLP DNA fingerprinting, genetic diversity, genetic relationship and identification of chicken breeds can be analysed.

Keywords

AFLP analysisbreed identificationgenetic relationshipindigenous chicken breeds
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 4 , Issue 1 , April 2007 , pp. 33 - 38
Copyright
Copyright © China Agricultural University and Cambridge University Press 2007

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Footnotes

First published in Journal of Agricultural Biotechnology 2006, 14(4): 498–502

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