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RAPD and large subunit nuclear rDNA sequence analyses of the entomogenous fungus Aschersonia

Published online by Cambridge University Press:  13 June 2008

Qiu Jun-Zhi ,
Huang Zhi-Peng ,
Pan Jie-Ru ,
Xie Xue-Qin ,
Zhu Yan-Ping ,
Fang Fang ,
Zhang Shao-Sheng  and
Guan Xiong
Qiu Jun-Zhi
Affiliation:
Key Lab of Biopesticide and Chemical Biology, Ministry of Education, Fuzhou 350002, China College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Huang Zhi-Peng
Affiliation:
Key Lab of Biopesticide and Chemical Biology, Ministry of Education, Fuzhou 350002, China
Pan Jie-Ru
Affiliation:
Key Lab of Biopesticide and Chemical Biology, Ministry of Education, Fuzhou 350002, China
Xie Xue-Qin
Affiliation:
Key Lab of Biopesticide and Chemical Biology, Ministry of Education, Fuzhou 350002, China
Zhu Yan-Ping
Affiliation:
Key Lab of Biopesticide and Chemical Biology, Ministry of Education, Fuzhou 350002, China
Fang Fang
Affiliation:
Key Lab of Biopesticide and Chemical Biology, Ministry of Education, Fuzhou 350002, China
Zhang Shao-Sheng
Affiliation:
Key Lab of Biopesticide and Chemical Biology, Ministry of Education, Fuzhou 350002, China
Guan Xiong*
Affiliation:
Key Lab of Biopesticide and Chemical Biology, Ministry of Education, Fuzhou 350002, China
*
*Corresponding author. Email: [email protected]
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Abstract

Eleven isolates within four species of the entomogenous fungus Aschersonia from China, the USA, Japan, the Philippines, Malaysia and Columbia were characterized using 17 RAPD primers. Genetic diversity among these strains of Aschersonia was found. The clustering results showed that the genetic variability among interspecies was more than that among intraspecies of Aschersonia. In the constructed phylogenetic tree, these isolates were not clustered according to their geographic origins or hosts. Furthermore, sequences of the divergent domain at the 5′-end of the large subunit (LSU) in nuclear rRNA from the mitosporic entomogenous fungi were employed to analyse the phylogenetic relationships of 11 Aschersonia isolates. The relationships of interspecies or intraspecies shown in the phylogenetic tree were almost consistent with the results of the morphological study. Different species isolated from different geographic origins could be clearly distinguished in the tree. But there were no close relationships among species isolated from the same family or order of insect hosts. The tree indicated that isolate Aa, belonging to A. aleyrodis, was the same as Aa992 and Aa3.4485. Moreover, results of RAPD analyses were consistent with those of LSU nuclear rDNA analysis for the same isolates tested, which consequently indicates that both methods can be independently applied for classification and identification of Aschersonia.

Keywords

AschersoniaRAPDLSU nuclear rDNAphylogeny
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 2 , Issue 2 , August 2005 , pp. 85 - 90
Copyright
Copyright © China Agricultural University and Cambridge University Press 2005

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