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Improving biocontrol effect of Pseudomonas fluorescens P5 on plant diseases by genetic modification with chitinase gene

Published online by Cambridge University Press:  12 February 2007

Xu Xiao-Jing ,
Zhang Li-Qun ,
Zhu You-Yong  and
Tang Wen-Hua
Xu Xiao-Jing
Affiliation:
Department of Plant Pathology, China Agricultural University, Beijing 100094, China
Zhang Li-Qun*
Affiliation:
Department of Plant Pathology, China Agricultural University, Beijing 100094, China
Zhu You-Yong
Affiliation:
Key Laboratory for Plant Pathology of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China
Tang Wen-Hua
Affiliation:
Department of Plant Pathology, China Agricultural University, Beijing 100094, China
*
*Corresponding author: Email: [email protected]
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Abstract

Pseudomonas fluorescens P5 was isolated in Yunnan Province and proved to be an effective biocontrol agent of various soil-borne plant diseases. The aim of the study was to enhance the biocontrol effect of this strain by introducing a chitinase gene. A 6.5 kb DNA fragment containing the chitinase gene chiB was inserted into pDSK519 vector to construct a new plasmid, pDSK51965. This plasmid was introduced into P. fluorescens P5 and resulted in a new strain named P5-1. Restriction enzyme digestion and chitin plate culture confirmed that P5-1 contained a functional chitinase gene. Compared with wild-type P5 in pot experiments, P5-1 had an increased effect against rice sheath blight and cotton damping-off caused by Rhizoctonia solani. It also increased the effect on suppression of wheat take-all caused by Gaeumannomyces graminis var. tritici.

Keywords

Pseudomonaschitinase genewheat take-allrice sheath blightcotton damping-off
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 2 , Issue 1 , April 2005 , pp. 23 - 27
Copyright
Copyright © China Agricultural University and Cambridge University Press 2005

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