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Expressing activator protein Ap36 in Bacillus thuringiensis and the function of the recombined strain on disease resistance

Published online by Cambridge University Press:  01 October 2008

Peng Dong-Hai ,
Zhou Chen-Fei ,
Qiu De-Wen ,
Zhou Kang ,
Ruan Li-Fang ,
Chen Shou-Wen ,
Yu Zi-Niu  and
Sun Ming
Peng Dong-Hai
Affiliation:
State Key Laboratory of Agricultural Microbiology, College of Life and Science Technology, Huazhong Agricultural University, Wuhan 430070, China These authors contributed equally to this work.
Zhou Chen-Fei
Affiliation:
State Key Laboratory of Agricultural Microbiology, College of Life and Science Technology, Huazhong Agricultural University, Wuhan 430070, China These authors contributed equally to this work.
Qiu De-Wen
Affiliation:
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Zhou Kang
Affiliation:
State Key Laboratory of Agricultural Microbiology, College of Life and Science Technology, Huazhong Agricultural University, Wuhan 430070, China
Ruan Li-Fang
Affiliation:
State Key Laboratory of Agricultural Microbiology, College of Life and Science Technology, Huazhong Agricultural University, Wuhan 430070, China
Chen Shou-Wen
Affiliation:
State Key Laboratory of Agricultural Microbiology, College of Life and Science Technology, Huazhong Agricultural University, Wuhan 430070, China
Yu Zi-Niu
Affiliation:
State Key Laboratory of Agricultural Microbiology, College of Life and Science Technology, Huazhong Agricultural University, Wuhan 430070, China
Sun Ming*
Affiliation:
State Key Laboratory of Agricultural Microbiology, College of Life and Science Technology, Huazhong Agricultural University, Wuhan 430070, China
*
*Corresponding author. E-mail: [email protected]
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Abstract

The gene ap36 encoding a protein elicitor from Alternaria sp. was fused downstream of the slh (S-layer homology) motif of Bacillus thuringiensis S-layer protein gene ctc. The recombinant gene was then transferred into B. thuringiensis plasmid-free derivative strain BMB171. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that the SLH–Ap36 fusion protein was expressed in B. thuringiensis BMB171. After tomato (Lycopersicum esculentum) leaves were treated for 90 min with the recombinant strain cultured at 28°C for 24 h, the activity of peroxidase and the amount of proline of tomato leaves were increased to 57.14% and 131.59%, respectively, compared to the control, and after the tomato leaves were treated with the cultured recombinant strain for 4 days, the activity of phenylalanine ammonia lyase was also higher than that in the control. Furthermore, tubers of treated potato (Solanum tuberosum) plants showed higher resistance to rot disease caused by Erwinia corotovora SCG1 compared to the control treatments.

Keywords

plant activator proteinBacillus thuringiensisS-layer homology motifinduction of defence responses
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 2 , August 2008 , pp. 121 - 126
Copyright
Copyright © China Agricultural University 2008

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Footnotes

First published in Journal of Agricultural Biotechnology 2008, 16(1): 142–147

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