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Development and preliminary application of gE enzyme-linked immunosorbent assay for detection of the antibody to gE protein of Pseudorabies virus in pigs

Published online by Cambridge University Press:  13 June 2008

Tang Yong ,
Chen Huan-Chun ,
Qin Ya-Li ,
He Qi-Gai ,
Jin Mei-Lli ,
Wu Bin  and
Liu Zheng-Fei
Tang Yong
Affiliation:
Laboratory of Animal Virology, College of Animal Medicinal Science, Huazhong Agricultural University, Wuhan 430070, China Department of Bioengineering, JiNan University, Guangzhou 51063, China
Chen Huan-Chun*
Affiliation:
Laboratory of Animal Virology, College of Animal Medicinal Science, Huazhong Agricultural University, Wuhan 430070, China
Qin Ya-Li
Affiliation:
Laboratory of Animal Virology, College of Animal Medicinal Science, Huazhong Agricultural University, Wuhan 430070, China
He Qi-Gai
Affiliation:
Laboratory of Animal Virology, College of Animal Medicinal Science, Huazhong Agricultural University, Wuhan 430070, China
Jin Mei-Lli
Affiliation:
Laboratory of Animal Virology, College of Animal Medicinal Science, Huazhong Agricultural University, Wuhan 430070, China
Wu Bin
Affiliation:
Laboratory of Animal Virology, College of Animal Medicinal Science, Huazhong Agricultural University, Wuhan 430070, China
Liu Zheng-Fei
Affiliation:
Laboratory of Animal Virology, College of Animal Medicinal Science, Huazhong Agricultural University, Wuhan 430070, China
*
*Corresponding author. Email: [email protected]
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Abstract

To differentiate pigs infected with Pseudorabies virus (PrV) from pigs vaccinated with gE-PrV, a glycoprotein E enzyme-linked immunosorbent assay (gE-ELISA) based on recombinant glycoprotein E (gE) (which was expressed by Escherichia coli, purified, denatured and renatured) was developed. By testing 115 serum samples, the diagnostic specificity and sensitivity of the developed gE-ELISA were evaluated to be 94.5% and 96.7%, respectively. Five serum samples were tested with plates from five lots, and the results had a coefficient of variation of less than 10%, showing good reproducibility of gE-ELISA. This gE-ELISA was compared with a commercial blocking ELISA by testing 356 serum samples. The agreement rate of the two assays was 92.13% (328/356). These results suggested that the gE-ELISA developed in our laboratory could be used in differentiating PrV-infected and gE-PrV-vaccinated pigs.

Keywords

Pseudorabies virusglycoprotein Eenzyme-linked immunosorbent assay (ELISA)
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 2 , Issue 2 , August 2005 , pp. 79 - 83
Copyright
Copyright © China Agricultural University and Cambridge University Press 2005

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References

Cook, D, Hill, H, Snyder, M et al. , (1990) The detection of antibodies to the glycoprotein X antigen of Pseudorabies virus. Journal of Veterinary Diagnostic Investigation 2 (1): 24–28.CrossRefGoogle Scholar
Elbers, AR, Braamskamp, J, Dekkers, LJ et al. , (2000) Aujeszky's disease virus eradication campaign successfully heading for last stage in The Netherlands. Veterinary Quarantine 22: 103–107.Google ScholarPubMed
Hampl, H (1984) Characterization of the envelope proteins of Pseudorabies virus. Journal of Virology 52: 58–59.CrossRefGoogle ScholarPubMed
Harbin Veterinary Research Institute of CAAS (1997) Veterinary Microbiology. Beijing: Chinese Agricultural Press, pp. 625–628 (in Chinese).Google Scholar
He, QG, Chen, HC, Qiu, DX et al. , (1999) Testing serum antibodies to pseudorabies with agglutination test. Chinese Veterinary Technology 29 (1): 7–8 (in Chinese with English abstract).Google Scholar
Li, WG and Gan, MH (1995) The current study of procine pseudorabies. Foreign Technology and Science of Farming 22 (1): 42–45 (in Chinese).Google Scholar
Liu, ZF (2002) Studies on TK and gE gene deleted vaccines of Pseudorabies virus. PhD thesis, Huazhong Agricultural University, Wuhan.Google Scholar
Marshak, DR, Kadonaga, JT, Burgess, RR et al. , (1996) Strategies for Protein Purification and Characterization: A Laboratory Course Manual. New York: Cold Spring Harbor Laboratory Press, pp. 148–151.Google Scholar
Mettenleiter, TC (1996) Immunobiology of pseudorabies (Aujeszky's Disease). Veterinary Immunology and Immunopathology 54: 221–229.Google Scholar
Mettenleiter, TC (2000) Pseudorabies (Aujeszky's disease) virus: State of the art. Veterinary Research 33 (1): 99–115.Google Scholar
Stegemam, A (1997) Aujeszky's disease (pseudorabies) virus eradication campaign in the Netherlands. Veterinary Microbiology 55 (1–4): 175–180.Google Scholar
Xiao, SB, Chen, HC, Fang, LR et al. , (2001) Cloning the major epitope domain of gE of Pseudorabies virus Ea strain and its expression In E. coli. Chinese Journal of Veterinary Science 21: 459–462 (in Chinese with English abstract).Google Scholar