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Creation of trivalent transgenic watermelon resistant to virus infection

Published online by Cambridge University Press:  12 February 2007

Niu Sheng-Niao ,
Huang Xue-Sen ,
Wong Sek-Man ,
Yu Jia-Lin ,
Zhao Fu-Xing ,
Li Da-Wei ,
Wang Sheng-You ,
Zhai Guang-Ming  and
Shi Fan-Sheng
Niu Sheng-Niao
Affiliation:
State Key Laboratory for Agro-biotechnology, China Agricultural University, Beijing, 100094, China
Huang Xue-Sen
Affiliation:
Shanxi Jinding Biology Seed Co. Ltd, Yuncheng, 044000, China
Wong Sek-Man
Affiliation:
National University of Singapore, Singapore, 117543
Yu Jia-Lin*
Affiliation:
State Key Laboratory for Agro-biotechnology, China Agricultural University, Beijing, 100094, China
Zhao Fu-Xing
Affiliation:
Shanxi Jinding Biology Seed Co. Ltd, Yuncheng, 044000, China
Li Da-Wei
Affiliation:
State Key Laboratory for Agro-biotechnology, China Agricultural University, Beijing, 100094, China
Wang Sheng-You
Affiliation:
Shanxi Jinding Biology Seed Co. Ltd, Yuncheng, 044000, China
Zhai Guang-Ming
Affiliation:
Shanxi Jinding Biology Seed Co. Ltd, Yuncheng, 044000, China
Shi Fan-Sheng
Affiliation:
Shanxi Jinding Biology Seed Co. Ltd, Yuncheng, 044000, China
*
*Corresponding author: Email: [email protected]
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Abstract

A trivalent vector containing genes of Watermelon mosaic virus (WMV) coat protein (CP), and replicase genes of Zucchini yellow mosaic virus (ZYMV) and Cucumber mosaic virus (CMV), was constructed for transformation of watermelon (Citrullus lanatus) plants, mediated by Agrobacterium tumefaciens. The integrated foreign genes were identified in the regenerated progenies by polymerase chain reaction (PCR) and Southern blots. The transformation efficiency was about 1.7‰. Resistance to virus infection was determined by mechanical inoculation in the greenhouse and by field trials. The transgenic watermelon lines showed different phenotypes of susceptible, resistant, immune or recovery from virus infections in the late growth stage. A relatively high level of resistance was shown by T3 plants of the line BH1-7. This result indicates the possibility of creating, by transgenic protocols, new varieties of watermelon resistant to viral infection.

Keywords

watermelonviral diseasestransformationresistance
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 2 , Issue 3 , December 2005 , pp. 179 - 185
Copyright
Copyright © China Agricultural University and Cambridge University Press 2005

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