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Cloning and sequence analysis of rolC gene affecting ginsenoside content in hairy roots of Panax ginseng C.A. Mey

Published online by Cambridge University Press:  12 February 2007

Zhao Shou-Jing ,
Li Chang-Yu ,
Liu Qing-Chang ,
Qian Yan-Chun  and
Shi Jing
Zhao Shou-Jing*
Affiliation:
College of Biological and Agricultural Engineering, Jilin University, Changchun 130025, China
Li Chang-Yu
Affiliation:
Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Jilin 132109, China
Liu Qing-Chang
Affiliation:
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China
Qian Yan-Chun
Affiliation:
College of Biological and Agricultural Engineering, Jilin University, Changchun 130025, China
Shi Jing
Affiliation:
College of Biological and Agricultural Engineering, Jilin University, Changchun 130025, China
*
*Corresponding author. E-mail: [email protected]
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Abstract

Ginseng hairy roots were induced from root explants of ginseng by the co-culture of Agrobacterium rhizogenes and ginseng (Panax ginseng C. A. Mey) roots through direct inoculation. The content of ginsenosides in the ginseng hairy roots after 4 weeks of culture reached the same level as in 3-year-old cultivated ginseng roots, and the content of one ginsenoside (Rb1) increased, showing that left transferred DNA (TL-DNA) has a function of influencing the biosynthesis of ginsenosides. Based on analysis of the RiA4TL-DNA sequence, the rolC gene affecting the synthesis of ginsenosides was amplified and cloned from the ginseng hairy roots by PCR. Comparison with a previously published nucleotide sequence showed that the homology was 99.9%.

Keywords

ginseng hairy rootsginsenosideTL-DNAcloning of gene rolCsequence analysis
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 1 , Issue 1 , April 2004 , pp. 61 - 65
Copyright
Copyright © China Agricultural University and Cambridge University Press 2004

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